LOCUS       sequence-33742-e         417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pEN12A-P1
FEATURES             Location/Qualifiers
     source          1..417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(128..232)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    274..373
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
       61 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      241 cccgaaaagt gccacctgac atcagctgga tggcaaataa tgattttatt ttgactgata
      301 gtgacctgtt cgttgcaaca aattgataag caatgctttc ttataatgcc aactttgtac
      361 aagaaagctg ggtgcatgcg gatcgtgctc atttcgggcg gcgaatctct cggcgtc
//
