LOCUS       sequence-33746-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, 
            Colecraft HM, Shaw RM
  TITLE     BIN1 localizes the L-type calcium channel to cardiac T-tubules.
  JOURNAL   PLoS Biol. 2010 Feb . 8(2):e1000312.
  PUBMED    20169111
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010 Feb . 8(2):e1000312."
COMMENT     Sequence Label: pcDNA3.2-hBIN1-V5
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    48..96
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     121..140
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(181..222)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    248..272
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
       61 aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta
      121 ttgtctcctt ccgtgtttca gttagcctcc cccgttttaa actcattact aaccggtacg
      181 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc
      241 tagatcaacc actttgtaca agaaagctgg gtatgggacc ctctcagtga agttctcggg
      301 gaagacgcca cggcacttct ccagctcctt gtgctggttc cagtcgctct ccttcacgcc
      361 catgagccag ccttcatcct gctcttcagg gttctggaag gggatcacca gcaccacatc
      421 accagccttg agctgcagct cgtctgtgtc agtggccgtg tagtcgtgct gggcctgtac
      481 cttgaacatg aaacctgggg gcaggtccaa gcgcccggcc ccactgccgc cctccacggt
      541 gccattcaca gttgctggga aggtctccac cacgacagca ggaagagagc tggaggctgc
      601 ttcacttgcc gccgtctccc ctggctcctg ggctccagcc gcaggttggg tcccacccgc
      661 cacctccgag gcctctgctg gctgagatgg ggacttgggg agggtggccc cgggcgtggc
      721 cccgccagcc ggctctggct cgtggttgac tctgatctcg ggggtggcgg caggggagcc
      781 atctggaggc gaagggctct tgttcccttt tgcaggcgcg ttgtcactgt tcttctttct
      841 gcgcagccgc g
//
