LOCUS       sequence-337467-       11623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses raptor tagged with mCherry and HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: pRK5-HA-mCherry-raptor
FEATURES             Location/Qualifiers
     source          1..11623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     724..748
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        958..976
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     958..975
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     989..1008
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1093..1102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1105..1131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1162..1171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1168..1875
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1313..1331)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1581..1600
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      8053..8062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8066..8200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8103..8122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8157..8176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8269..8626
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8269..8460
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8292..8312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8477..8612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8646..8663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8646..8662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8655..8677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8875..9330
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8962..8981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9172..9193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9347..9366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9466..9488
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9526..9544)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9612..9716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9717..9785,9786..10577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9935..9954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10748..11336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11237..11256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11490..11507
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
      121 tacgaattaa ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
      841 gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt
      901 ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      961 taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag
     1021 gtccaactgc acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc
     1081 aaggccattc gcgccaccat gggctacccc tatgatgtgc ctgactacgc agatcgcaat
     1141 ggtggtggtg gtggaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     1201 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1261 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1321 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1381 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1441 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1501 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1561 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1621 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1681 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1741 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1801 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1861 gacgagctgt acaagtccgg actcagatct ggtggagggc ctggtggagg tgggcctgga
     1921 ggtggaggac ctggtggtgg tgggtcgacg atggagtccg aaatgctgca atcgcctctt
     1981 ctgggcctgg gggaggaaga tgaggctgat cttacagact ggaacctacc tttggctttt
     2041 atgaaaaaga ggcactgtga gaaaattgaa ggctccaaat ccttagctca gagctggagg
     2101 atgaaggatc ggatgaagac agtcagtgtt gccttagttt tgtgcctgaa tgttggtgtg
     2161 gaccctcccg atgtggtgaa gaccacgccc tgtgcacgct tggaatgctg gatcgatcct
     2221 ctgtcgatgg gtcctcagaa agctctggaa accatcggtg caaatttaca gaagcagtac
     2281 gagaactggc agccaagggc ccggtacaag cagagccttg acccaactgt ggatgaagtc
     2341 aagaagctct gcacgtcctt acgtcgcaac gccaaggagg agcgagtcct ctttcactac
     2401 aatggccacg gggtgccccg gcccacagtc aacggggagg tctgggtctt caacaagaac
     2461 tacacgcagt acatccctct gtccatatat gacctgcaga cgtggatggg cagcccgtcg
     2521 atcttcgtct acgactgctc caatgctggc ttgatcgtca agtccttcaa gcagttcgca
     2581 ctacagcggg agcaggagct ggaggtagct gcaatcaacc caaatcaccc tcttgctcag
     2641 atgcctttgc ctccgtcgat gaaaaactgc atccagctgg cagcctgcga ggccaccgag
     2701 ctgctgccca tgatccccga cctcccggct gacctattca cctcctgcct caccaccccc
     2761 atcaagatcg ccctgcgctg gttttgcatg cagaaatgtg tcagtctggt gcctggcgtc
     2821 acactggatt tgatagaaaa gatccctggc cgcctgaacg acaggaggac gcccctgggt
     2881 gaactgaact ggatcttcac agccatcaca gacaccatcg cgtggaacgt gctcccccgg
     2941 gatctcttcc aaaagctctt cagacaggac ttgctggtgg ctagtctgtt tcgaaatttt
     3001 ttattggcgg aaaggattat gaggtcgtat aactgcactc ccgtcagcag cccgcgtctg
     3061 ccgcccacgt acatgcacgc catgtggcaa gcctgggacc tggctgttga catctgtctg
     3121 tctcagctgc cgacgatcat cgaggaaggc actgcgtttc ggcacagccc gttcttcgcc
     3181 gagcagctga ccgcattcca ggtgtggctc accatgggcg tggagaaccg aaacccaccc
     3241 gaacagctgc ccatcgtcct gcaggtgctg ttaagccaag tgcaccggct gagagcattg
     3301 gacttgcttg gaagattttt ggacctgggt ccctgggcag tgagcctggc cttgtctgtc
     3361 ggcatcttcc cctacgtgct gaagctgctc cagagctcgg cccgagagct gcggccactt
     3421 ctcgttttca tctgggccaa gatcctcgca gtggacagct cgtgccaggc ggacctcgtg
     3481 aaggacaacg gccacaagta cttcctgtcg gtcctggcgg acccctacat gccagctgaa
     3541 caccggacca tgacggcttt cattctcgcc gtgatcgtca acagctatca cacggggcag
     3601 gaagcctgcc ttcagggaaa cctcattgcc atctgcctgg agcagctcaa cgacccgcac
     3661 cccttgctgc gccagtgggt ggccatctgc ctcggcagga tctggcagaa cttcgactcg
     3721 gcgaggtggt gcggcgtgag ggacagcgct catgagaagc tctacagcct cctctccgac
     3781 cccattcccg aggtccgctg cgcagcggtc ttcgcccttg gcacgttcgt gggcaactct
     3841 gcagagagga cggaccactc caccaccatc gaccacaacg tggccatgat gctggcccag
     3901 ctggtcagcg acgggagccc catggtccgg aaggagctgg tggtggctct gagtcatctt
     3961 gtggttcagt atgaaagcaa tttctgcacc gtggccctgc agttcataga agaggaaaag
     4021 aactacgcct tgccttctcc agcaaccaca gagggaggga gtttgacccc agtgcgagac
     4081 agcccgtgca cccccagact tcgttctgtg agctcctatg gaaacatccg tgctgtcgcc
     4141 acagccagga gcctcaacaa atctttgcag aacctgagtt tgacagagga atctggtggc
     4201 gcggtggcgt tctcccccgg aaacctcagc accagcagca gcgccagcag caccctgggc
     4261 agccccgaga atgaggagca tatcctgtcc ttcgagacca tcgacaagat gcgccgcgcc
     4321 agctcctact cctccctcaa ctccctcatc ggagtttcct ttaacagtgt ttacactcag
     4381 atttggagag tcctgctgca cctggctgct gacccctatc cagaggtctc ggacgtggcc
     4441 atgaaagtac tcaacagcat cgcctacaag gccaccgtga acgcccggcc gcagcgcgtc
     4501 ctggacacct cctccctcac gcagtcggcc cccgccagcc ccaccaacaa gggcgtgcac
     4561 atccaccagg cggggggctc ccctccggcg tccagcacca gcagctccag cctgaccaac
     4621 gatgtggcca agcagccggt cagccgagac ttgccttctg gccggccggg caccacaggc
     4681 cccgctgggg cgcagtacac ccctcactcc caccagttcc cccggacacg gaagatgttc
     4741 gacaagggcc cagagcagac tgcggacgac gcggacgatg ctgctggaca caaaagtttc
     4801 atctccgcca cggtgcagac ggggttctgc gactggagcg cccgctattt tgcccagccc
     4861 gtcatgaaga tcccagaaga gcacgacctg gagagtcaga tccgcaagga gcgggagtgg
     4921 cggttcctgc gaaacagccg tgtcaggagg caggcccagc aagtcattca gaagggcatt
     4981 acgagattgg acgaccaaat atttctgaac aggaaccccg gcgtcccctc tgtggtgaaa
     5041 ttccacccct tcacgccgtg catcgctgta gccgacaagg acagcatctg cttttgggac
     5101 tgggagaaag gggagaagct ggattatttc cacaatggga accctcggta cacgagggtc
     5161 actgccatgg agtatctgaa tggccaggac tgctcgcttc tgctgacggc cacagacgat
     5221 ggtgccatca gggtctggaa gaattttgct gatttggaaa agaacccaga gatggtgacc
     5281 gcgtggcagg ggctctcgga catgctgcca acgacgcgag gagctgggat ggtggtggac
     5341 tgggagcagg agaccggcct cctcatgagc tcaggagacg tgcggatcgt ccggatctgg
     5401 gacacagacc gtgagatgaa ggtgcaggac atccctacgg gcgcagacag ctgtgtgacg
     5461 agtctgtcct gtgattccca ccgctcactc atcgtggctg gcctcggtga cggctccatc
     5521 cgcgtctacg acagaaggat ggcactcagc gaatgccgcg tcatgacgta ccgggagcac
     5581 acagcctggg tggtgaaggc ctccctgcag aagcgtcccg acggccacat cgtgagtgtg
     5641 agcgtcaatg gagatgtgcg catctttgat ccccggatgc ctgagtcggt aaatgtgctt
     5701 cagatcgtga aggggctgac ggccctggac atccaccccc aggcggacct gatcgcatgt
     5761 ggctccgtca atcagttcac cgccatctac aacagcagcg gagagctcat caacaacatc
     5821 aagtactacg acggcttcat gggccagcgg gtcggcgcca tcagctgcct ggccttccac
     5881 ccgcactggc ctcacctggc cgtgggaagc aacgactact acatctccgt gtactcggtg
     5941 gagaagcgtg tcagatagcg gcgtgacccg ggcccaccag gccacggccg cctgctgtac
     6001 atagtgaagc tgtcactcgc cggggcacgg ggcgtcggct gctgcggccc cgcagtgtga
     6061 acgttggctg ctgccttagc tgctgatgac ggcaggaggg ccctgctact cgcttttgtc
     6121 tgtcttcgct gtcgtgtctg gaatgtcagg gaaggggagg gctcgggttg acggtggctt
     6181 cccactgagc accagcatcc aggtgcaccc ccgcggccac ggcgcctctg tccctctcct
     6241 gttctgtgtt tctctgagac gctgaaaggg gaaacacctc actttatttc catgtaatca
     6301 gagcattagc tgcagaaaaa cccccagaca gagccctggc ggagaggcag gcgctggggc
     6361 tcctacgggt ccctggggca gctgtcccca tcaggccaag agcgagcgag aggcgctgcc
     6421 ccagccaggc ccaccacctc tcacagtcag tgcacgcaag cagggacatt tcctagccag
     6481 ctgggggaca ctggaaattc gggaaaccaa gagagaggaa gaaggagacg cccctccaac
     6541 tggcgggtgt gaaggaagcc gcccaggggt ccgggctgtc cttggccgct ggcagcatca
     6601 ctgagcagga agcgcacagc ccaccctccc cgcacctcca ggtctctgga ctccagtttt
     6661 ggcccctctc acacagagct gtcagcaggg gccgctgtgg cggtgcacag gggaggcagg
     6721 tccttggcga ggtagcccct gccttaatcc acggggctcc tttccctccg aagggctgct
     6781 cttccccaca ggcgcgggga cagcagcccg acctgtggtc tccatgcctg tgccctcaca
     6841 caggtgtagc acacgcatgt gcagatggca ccacggccgg cacctggggg cacacacatg
     6901 caggcggcgt ggtctccctg ctctgtcccc acacgttcct cacatacagg caagaggcac
     6961 tgccgggtcc cggacggctc cgggtgacac cagccccgtc tccagccttg agccgcccat
     7021 gctgatgcga cctcggctga cagctgggcc tgtggtgcag acaggagctg tgtggacagt
     7081 cccgcccagg aggggccgca gggcgtgtat gagcagtttt gcaaacagaa cacaaccaca
     7141 atgatggtat tttgaaaagt gttctttccg tgttcgtcgg gaatcaggat tattgagagg
     7201 tgaaggagcc aggtggcttc attctggcgg tgagaggccc atgaccacgg gagtgagagc
     7261 tggtgtggcg aggcccggct ctcctgcggt gtggctggtg gcctgccgtg gccaagagca
     7321 tcttctgggt ggatggaacc ctgcctggtc acatttggcc agagacacac ctggccctca
     7381 gggggctgag ctggagactg agctggggct ggccgggacg tgacaaggca ggacagaggc
     7441 ggcccctccg ctgctccttt ttggaatgcg agctcccacc agaagaaggt tccggcacga
     7501 atcccatccc cacgtctggg ccgagaaagc agcccgggtc cggaaggtgt agagagtccc
     7561 ggcctcactc agctcacagg gcgtgccagg cggcaacacc agaatcttcc agaagcccag
     7621 ctccacccgc acacgcagct tcccatccag tccttcaact caattcttac ccaacacgcg
     7681 tttccgtttg ttttgagaca aaatcaccac ctgtcaaaag gcaggtggct ccagaggggt
     7741 caagaccccc ccccccgccc ccgctccacc ctggagccca cccccatggg cactgcgtgc
     7801 cgcctgcacg tgggctgtct tcacaggtct gatgtgaaaa ttcaatcacg acgttaaccg
     7861 gctcgagaga gcgccggcct agaggctcat tatctattta ttttaccaaa cgcgaattga
     7921 gacggacttt gacaaaacac gaaatggtaa tgtgaagcta agagcagaga gtgaccaaca
     7981 gtaaacaaca cgcgcagact caaaaaaaaa aaaaaaaggg cggccgctaa gtaagtaagg
     8041 atccccagct tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata
     8101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8161 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc
     8221 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
     8281 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8401 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8461 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8521 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8581 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc
     8641 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8701 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8761 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     8821 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     8881 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8941 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     9001 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     9061 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     9121 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     9181 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     9241 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9301 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     9361 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9421 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9481 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     9541 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9961 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10021 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10081 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10141 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10201 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10261 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10321 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10381 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10441 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10501 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10561 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10621 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10681 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10861 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11101 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    11581 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat
//
