LOCUS       sequence-337478-        4329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing GATA6 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-GATA6
FEATURES             Location/Qualifiers
     source          1..4329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1069..1098
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1886..1985)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2002..2021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2003..2021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2026..2042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2026..2042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2144..2163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2155..2964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2222..2241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3111..3699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3600..3619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3853..3870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4029..4056)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4148..4234)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4283..4305
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4297..4314
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4298..4314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggccctgac cgacggcgga
      121 tggtgtctcc ctaaaagatt cggcgccgct ggcgctgatg cttctgacag cagagccttc
      181 cccgctaggg aacccagcac accacctagc cccatcagca gctcaagctc tagctgtagc
      241 agaggcggag agagaggacc tggaggcgct tctaactgcg gcacacctca gctggataca
      301 gaagccgccg ccggaccacc agccagatct cttttactta gcagctacgc cagccaccct
      361 tttggcgctc ctcatggacc ctctgctcct ggtgtggccg gacctggcgg aaacctgagc
      421 tcttgggagg accttctgct gtttaccgac ctggaccagg ctgccaccgc tagcaagctt
      481 ctgtggagca gcaggggcgc taagctgagc ccttttgccc ctgagcagcc cgaggagatg
      541 taccagaccc tggctgcttt aagctctcag ggacctgccg cttatgacgg agcccctggt
      601 ggatttgttc actcagcggc agcagccgca gctgctgcag ccgctgccag ctcacctgtg
      661 tatgtgccta ccacaagagt gggcagcatg ttacctggac ttccttacca tctgcagggc
      721 agcggaagcg gccctgctaa ccatgccgga ggagctggag ctcaccccgg atggcctcag
      781 gcttctgcag attctcctcc ttatggatct ggaggaggag cagctggagg gggagctgca
      841 ggaccaggtg gagccggaag cgcagcagca catgtgtctg ccagatttcc ctatagccct
      901 agccctccta tggccaatgg cgctgctaga gaacccggag gatatgctgc ggcaggctct
      961 ggcggcgctg gcggagtttc tggaggtgga tcttcactgg ccgctatggg aggaagagag
     1021 cctcagtact cttctctgag cgccgctaga ccactgaacg gcacctatca tcaccaccac
     1081 catcaccatc atcatcaccc cagcccttac tccccttatg tgggagcccc ccttacaccc
     1141 gcttggcctg ccggcccttt cgagacacct gtgctgcaca gccttcagtc tagagctggc
     1201 gcacctttac cagtgcctag aggcccctct gccgacttgc tggaggatct gagcgagagc
     1261 agagagtgcg tgaactgtgg cagcatccag acacccctgt ggagaagaga cggcaccggc
     1321 cactacctgt gcaacgcttg cggcctgtac agcaagatga atgggctgag cagacccctg
     1381 atcaagcccc agaagagggt gcccagcagc agacggctgg gactgagctg cgccaactgt
     1441 cataccacaa caaccacact gtggcggaga aacgccgagg gcgagcccgt gtgtaacgcc
     1501 tgcggccttt acatgaagct gcacggcgtg cccagacctc tggccatgaa gaaggaggga
     1561 atccagacca gaaagagaaa gcccaagaac atcaacaaga gcaagacctg cagcggcaac
     1621 agcaacaaca gcatccccat gacccccacc agcacatcta gcaacagcga cgactgtagc
     1681 aagaacacat cacctaccac ccagcccaca gctagcggag ccggcgcccc cgtgatgaca
     1741 ggcgccggag agtccacaaa tcccgagaat agcgaactga agtactctgg acaggacgga
     1801 ctgtatatcg gcgtgagcct ggcttctccc gccgaggtga ccagctctgt cagacctgac
     1861 tcttggtgtg ccctcgccct ggcctgccca actttcttgt acaaagttgg cattataaga
     1921 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1981 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2041 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2101 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2161 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2221 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2281 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2341 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2401 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2461 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2521 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2581 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2641 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2701 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2761 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2821 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2881 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2941 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3001 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3061 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3901 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3961 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     4021 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4081 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4141 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4201 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4261 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4321 ctcgggccc
//
