LOCUS       sequence-337491-        9366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018267
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     387..404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(557..562,563..580,581..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     600..622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     619..635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     619..635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             1321..1343
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3066..3086
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3087..3110
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    3136..3192
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3193..3210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3193..3209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3202..3224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3418..3437)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3537..3559
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3597..3615)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             4401..5600
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(5831..6979)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7251..7622)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7655..7764
                     /note="incP origin of transfer"
     rep_origin      7917..8548
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      join(9011..9366,1..233)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctagcttccc
      661 gcttggtcgg gccgcttcgc gagggcccgt tgacgacaac ggtgcgatgg gtcccggccc
      721 cggtcaagac gatgccaata cgttgcgaca ctacgccttg gcacttttag aattgcctta
      781 tcgtcctgat aagaaatgtc cgaccagcta aagacatcgc gtccaatcaa agcctagaaa
      841 atataggcga agggacgcta ataagtcttt cataagaccg cgcaaatcta aaaatatcct
      901 tagattcacg atgcggcact tcggatgact tccgagcgag cctggaacct cagaaaaacg
      961 tctgagagat accgcggatc tcacacagga aatttcgcaa cgcaaaatgc tgaatttagc
     1021 aggcaagttt cctgataaag ggccctgtga gtgcgacatt cgacgcgcga aagcgagttg
     1081 acggcgcccg aaattttctt agagttcgtt caagcttcgg tttcggggag ccgctaaggg
     1141 atgcggaccc agccgggtaa gtcgccggga actatggaat aatcctaatt tctgtgcggc
     1201 aggcgcgagc tgccggagcg aatatcatcc ctgtcgcaaa cagcaggtca atcaacggtg
     1261 ggtagcgggc tgctatccag cggcggataa gacttgtcgg aggaatcctc tagaaataat
     1321 tttgtttaac tttaagaagg agatatacca tgacgtttgg gaccaagggc ttcctggcgg
     1381 gcatctcgat ggtcgccatg ctggcgacgg cgacctcggg ggtccgtgcc aacgactccc
     1441 tcctcaaggc tcagagcgac tcgtcgcaat gggcggtcgc ggggcatgat tacgggaaca
     1501 cccgattctc cccgctgaag cagatcaaca ccgagaatgc gggcaaactc accctggcgt
     1561 acagtttttc gctcgcctcg ctgcgttcca acgaatcgag ccccattgtc gtgggcaaca
     1621 cgctctacgt ctcgtcgagc tggggcccca agtatgtgta cgctctggac gccgcgaccg
     1681 gtgtacgcaa gtggacctat gagccggaca ttccggagga cgttctgcag tacgcttgtt
     1741 gcgacgtcaa caaccgcggc gtcagctacg ccgacgggaa gatcttcgtc ggccggctgg
     1801 atggcaaact caccgccctt gacgccgcca ccgggaaggc cctgtggacc gcgaaggtcg
     1861 tcgattataa gcagggttcg gtcatcacca gccccccgct cgtggtgcgc gataaggtta
     1921 tcaccggttt cggcggcggc gagtacggtg tccgcggctc cctccaggcc ttcgacatca
     1981 acacgggcaa acagctgtgg cagacctaca ccgtcccgag tccggatgag ccgggcggcg
     2041 acacctggaa gggcgacagc gcccagcacg gaggcggcgc cgcgtggctc gtcggctcct
     2101 acgatccgaa aacggacacc gtctactggg gcacctccaa cccgggtccc tggaataccg
     2161 ccgtccggtc gacaggcgat ggcaacttcg gcaagctgac gaacctatac accgcctcca
     2221 cggtcgcctt cgaccccaac acgggtaaga tcaagtggca catccagacg acgccggccg
     2281 acgcgtggga ctatgacggc gtcaacgaag cggtcctcgc cgacctcaag atcggcgggt
     2341 cgaccgtgcc ggcgctgatg aaggccgacc gcaacgggta cttcttcgtc gcgaatcgcg
     2401 agaccggcaa ggtcctctcg gcagaaaagt acgtgttctc aaactgggcg aagaagtggg
     2461 acatcgcgac catgagggcg gtcgaagacc ccgacaagcg gccgggcccc aaccatccgg
     2521 cgaaggacat ctgcccgaac ctgattggcg gcaagaactg gcagccgatg tcgttcaacc
     2581 cacagacggg cctggtttat atcccgagca acaacgtctg catggattgg tccgtcagcg
     2641 acgtcgcgta taagcgcggc gtcttctacc tcggggccga gttcccgacg aaggaaggcc
     2701 cggggggctt cctgggggag ctagtcgcct gggatcccgt cgcgcagaag aaggtctgga
     2761 gcatcaagga ggacctcccc ttcaacgggg gcacactgac gacaggcggc gggctggtct
     2821 tcgccggcaa cattcatggc gatttccgcg cgatcgatgc caaatcgggc aaggtcctct
     2881 ggtcgcggaa cctggggagc ggcatcggcg cgggcccggt gacctaccag gttgacggca
     2941 agcagtacgt ggccatcgtt gtgggccgca ccgccgcgct cccggcgttc ttgggcgagg
     3001 tcggcaagaa gatgacggcc gccgccccag agggcggatc cctgtttgtc ttctccgtcc
     3061 aggccgaaaa cctctatttt cagggacatc atcaccatca ccatcaccac tgacagctat
     3121 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     3181 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3241 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3301 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     3361 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3421 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     3481 gccctgacgg gcttgtatgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     3541 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     3601 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agcacccttt
     3661 ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc
     3721 gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc
     3781 cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg
     3841 tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca
     3901 ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc
     3961 gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag
     4021 gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg
     4081 tcggctctcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg
     4141 agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt
     4201 tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg
     4261 gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac
     4321 ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga
     4381 ggctggtccg gaggccagac gtgaaaccca acatacccct gatcgtaatt ctgagcactg
     4441 tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg
     4501 atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt
     4561 tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc
     4621 caatcttgct cgtctcgctg gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc
     4681 ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg
     4741 cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg
     4801 gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga
     4861 tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt
     4921 tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc
     4981 gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg
     5041 ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg
     5101 tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg
     5161 catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc
     5221 tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc
     5281 ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     5341 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     5401 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     5461 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     5521 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     5581 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     5641 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     5701 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     5761 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     5821 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     5881 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     5941 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     6001 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     6061 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     6121 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     6181 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     6241 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     6301 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     6361 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     6421 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     6481 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     6541 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     6601 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     6661 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     6721 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     6781 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     6841 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     6901 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     6961 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     7021 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     7081 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     7141 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     7201 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     7261 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     7321 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     7381 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     7441 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     7501 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     7561 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     7621 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
     7681 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     7741 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     7801 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     7861 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg
     7921 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     7981 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     8041 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     8101 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     8161 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     8221 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     8281 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     8341 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     8401 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     8461 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     8521 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     8581 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc aagctgacca cttctgcgct
     8641 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8701 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8761 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8821 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8881 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8941 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9361 agcgaa
//
