LOCUS       sequence-337512-        6269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the purifcation of NDP52 protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, 
            Hurley JH
  TITLE     Reconstitution of cargo-induced LC3 lipidation in mammalian 
            selective autophagy.
  JOURNAL   Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 
            10.1126/sciadv.abg4922. Print 2021 Apr.
  PUBMED    33893090
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-04-23"; volume: "7(17). pii: 7/17/eabg4922. doi"; pages:
            " 10.1126/sciadv.abg4922. Print 2021 Apr"
COMMENT     Sequence Label: GST-NDP52
FEATURES             Location/Qualifiers
     source          1..6269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(173..178,179..196,197..203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     235..251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             247..774
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(267..284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(267..283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(876..881,882..897,898..904)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    912..928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             951..1604
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1562..1584
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(3012..3034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3179..3197)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3265..3369
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3370..3438,3439..4230)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3588..3607)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4401..4989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4890..4909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5143..5160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5233..5310
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(5311..6269,1..124)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVKAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5330..5349)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
       61 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      121 gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccccag gctttacact
      181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa
      301 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      421 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
      481 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
      541 cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
      601 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
      661 cagacgcgaa ttatttttga tggcgttgga attagcttat cgactgcacg gtgcaccaat
      721 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact
      781 gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat
      841 cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat
      901 aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagtattc atgtccccta
      961 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
     1021 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
     1081 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
     1141 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
     1201 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
     1261 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
     1321 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
     1381 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
     1441 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
     1501 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
     1561 agggctggca agccacgttt ggtggtggcg accatcctcc aaaaatcgat atggaggaga
     1621 ccatcaaaga tccccccaca tcagctgtct tgctggatca ctgtcatttc tctcaggtca
     1681 tctttaacag tgtggagaag ttctacatcc ctggagggga cgtcacatgt cattatacct
     1741 tcacccagca tttcatccct cgtcgaaagg attggattgg catctttaga gtggggtgga
     1801 agacaacccg tgagtattac accttcatgt gggttacttt gcccattgac ctaaacaaca
     1861 aatcagctaa acagcaggaa gtccaattca aagcttacta cctgcccaag gatgatgagt
     1921 attaccagtt ctgctatgtg gatgaggatg gtgtggtccg gggagcaagt attcctttcc
     1981 aattccgtcc agaaaatgag gaagacatcc tggttgttac cactcaggga gaggtggaag
     2041 agattgagca gcacaacaag gagctttgca aagaaaacca ggagctgaag gacagctgta
     2101 tcagcctcca gaagcagaac tcagacatgc aggctgagct ccaaaagaag caggaggagc
     2161 tagaaaccct acagagcatc aataagaagt tggaactgaa agtgaaagaa cagaaggact
     2221 attgggagac agagctgctt caactgaaag aacaaaacca gaagatgtcc tcagaaaatg
     2281 agaagatggg aatcagagtg gatcagcttc aggcccagct gtcaactcaa gagaaagaaa
     2341 tggagaagct tgttcaggga gatcaagata agacagagca gttagagcag ctgaaaaagg
     2401 aaaatgacca cctctttctc agtttaactg aacagaggaa ggaccagaag aagctcgagc
     2461 agacagtgga gcaaatgaag cagaatgaaa ctactgcaat gaagaaacaa caggaattaa
     2521 tggatgaaaa ctttgacctg tcaaaaagac tgagtgagaa cgaaattata tgtaatgctc
     2581 tgcagagaca gaaagagaga ttggaaggag aaaatgatct tttgaagagg gagaacagca
     2641 gattgctcag ttacatgggt ctggatttta attctttgcc gtatcaagta cctacttcag
     2701 atgaaggagg cgcaagacaa aatccaggac ttgcctatgg aaacccatat tctggtatcc
     2761 aagaaagttc ttcccccagc ccgctctcca tcaagaaatg ccctatctgc aaagcagatg
     2821 atatttgtga tcacaccttg gagcaacagc agatgcagcc cctttgtttc aattgtccaa
     2881 tttgtgacaa gatcttccca gctacagaga agcagatctt tgaagaccac gtgttctgcc
     2941 actctcttta agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat
     3001 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3061 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3121 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc
     3181 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3241 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3301 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3361 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3421 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3481 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3541 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3601 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3661 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3721 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3781 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3841 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3901 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3961 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4021 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4081 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4141 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4201 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4261 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4321 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5101 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5161 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5221 cgcataaatt ccgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc
     5281 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
     5341 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
     5401 tctgcgaaaa cgcgggaaaa agtgaaagcg gcgatggcgg agctgaatta cattcccaac
     5461 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     5521 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     5581 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     5641 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     5701 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     5761 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
     5821 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     5881 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     5941 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     6001 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     6061 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     6121 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
     6181 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     6241 caggcggtga agggcaatca gctgttgcc
//
