LOCUS       sequence-33753-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, 
            Bartel DP
  TITLE     Crystal structure of the catalytic core of an RNA-polymerase 
            ribozyme.
  JOURNAL   Science. 2009 Nov 27. 326(5957):1271-5.
  PUBMED    19965478
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Nov 27. 326(5957):1271-5."
COMMENT     Sequence Label: pH307HP
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    264..320
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(333..349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(333..349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(346..368)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    357..373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(381..387,388..405,406..411))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    426..447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(564..581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(815..834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggacgaaa cggtacccgg taccgtctcc agtaggaaca ctatactact ggataatcaa
       61 agacaaatct gcccgaaggg cttgagaaca tacccattgc actccgggta tgcagaggtg
      121 gcagcctccg gtgggttaaa acccaacgtt ctcaacaata gtgaggccgg catggtccca
      181 gcctcctcgc tggcgccggc tgggcaacat tccgagggga ccgtcccctc ggtaatggcg
      241 aatgggaccc acaaatggaa gaggaattcg agctcggtac ccggggatcc tctagagtcg
      301 acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      781 tcaagtcaga ngtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      961 taggtcg
//
