LOCUS       sequence-337542-        9820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Teo SLY, Rennick JJ, Yuen D, Al-Wassiti H, Johnston APR, Pouton CW
  TITLE     Unravelling cytosolic delivery of cell penetrating peptides with a 
            quantitative endosomal escape assay.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3721. doi: 10.1038/s41467-021-23997-x.
  PUBMED    34140497
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3721. doi"; pages: " 
            10.1038/s41467-021-23997-x"
COMMENT     Sequence Label: pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2973..3518
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    join(4694..5268,5269..5271,5272..5280)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4861..4878)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5088..5107
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5293..5892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5293..5312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5789..5809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5899..6487
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5952..5972)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6370..6381)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6561..6794
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6866..6987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6903..6922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6957..6976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7027..7162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7195..7211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7208..7230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7219..7235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7243..7249,7250..7267,7268..7273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7288..7309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7426..7443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7597..8185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8356..9147,9148..9216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8979..8998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9217..9321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9389..9407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9445..9467)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9567..9586
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9780..9802
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9794..9811
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9795..9811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagaggcca ccatgatggt cttcacactc gaagatttcg
     2521 ttggggactg ggaacagaca gccgcctaca acctggacca agtccttgaa cagggaggtg
     2581 tgtccagttt gctgcagaat ctcgccgtgt ccgtaactcc gatccaaagg attgtccgga
     2641 gcggtgaaaa tgccctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
     2701 ccgaccaaat ggcccagatc gaagaggtgt ttaaggtggt gtaccctgtg gatgatcatc
     2761 actttaaggt gatcctgccc tatggcacac tggtaatcga cggggttacg ccgaacatgc
     2821 tgaactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     2881 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc acccccgacg
     2941 gctccatgct gttccgagta accatcaaca gtatggacaa agactgcgaa atgaagcgca
     3001 ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc
     3061 gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag
     3121 ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact
     3181 ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc
     3241 agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag
     3301 aggtcatcag ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg
     3361 tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc
     3421 agggcgacct ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg
     3481 cccacgaggg ccacagactg ggcaagcctg ggctgggtcc tgcaggcgga tccatggatg
     3541 atgatatcgc cgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg
     3601 gcgacgatgc cccccgggcc gtcttcccct ccatcgtggg gcgccccagg caccagggcg
     3661 tgatggtggg catgggtcag aaggattcct atgtgggcga cgaggcccag agcaagagag
     3721 gcatcctcac cctgaagtac cccatcgagc acggcatcgt caccaactgg gacgacatgg
     3781 agaaaatctg gcaccacacc ttctacaatg agctgcgtgt ggctcccgag gagcaccccg
     3841 tgctgctgac cgaggccccc ctgaacccca aggccaaccg cgagaagatg acccagatca
     3901 tgtttgagac cttcaacacc ccagccatgt acgttgctat ccaggctgtg ctatccctgt
     3961 acgcctctgg ccgtaccact ggcatcgtga tggactccgg tgacggggtc acccacactg
     4021 tgcccatcta cgaggggtat gccctccccc atgccatcct gcgtctggac ctggctggcc
     4081 gggacctgac tgactacctc atgaagatcc tcaccgagcg cggctacagc ttcaccacca
     4141 cggccgagcg ggaaatcgtg cgtgacatta aggagaagct gtgctacgtc gccctggact
     4201 tcgagcaaga gatggccacg gctgcttcca gctcctccct ggagaagagc tacgagctgc
     4261 ctgacggcca ggtcatcacc attggcaatg agcggttccg ctgccctgag gcactcttcc
     4321 agccttcctt cctgggcatg gagtcctgtg gcatccacga aactaccttc aactccatca
     4381 tgaagtgtga cgtggacatc cgcaaagacc tgtacgccaa cacagtgctg tctggcggca
     4441 ccaccatgta ccctggcatt gccgacagga tgcagaagga gatcactgcc ctggcaccca
     4501 gcacaatgaa gatcaagatc attgctcctc ctgagcgcaa gtactccgtg tggatcggcg
     4561 gctccatcct ggcctcgctg tccaccttcc agcagatgtg gatcagcaag caggagtatg
     4621 acgagtccgg cccctccatc gtccaccgca aatgcttcta ggaattcgaa tttaaatcgg
     4681 atccgcggcc gcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     4741 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4801 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4861 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4921 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4981 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5041 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5101 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5161 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     5221 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     5281 atggcgtccg gaatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5341 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5401 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5461 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5521 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5581 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5641 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5701 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5761 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5821 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5881 cccggtgcct aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6301 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6481 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6541 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     6601 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6661 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6721 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6781 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6841 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6901 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6961 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     7021 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7081 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7141 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     7201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9361 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9421 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9481 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9541 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9601 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     9661 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9721 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9781 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
