LOCUS       sequence-337557-       11969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKlux3
FEATURES             Location/Qualifiers
     source          1..11969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..72)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    300..321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(336..341,342..359,360..366)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    374..390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     379..401
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     398..414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     398..414
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     433..453
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        435..453
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     482..498
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     483..499
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             537..1979
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1992..2915
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2964..4046
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4061..5044
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5223..6335
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(6415..7497)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7459..7478
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7498..7575)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(7596..7615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7597..7615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7625..7642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7625..7641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7634..7656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(8350..9012)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      9013..9784
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(join(11109..11900,11901..11969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11732..11751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
      121 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
      181 cagtttttat gcatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      301 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      361 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      421 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccccc
      481 ctcgaggtcg acggtatcga taagcttgat atcgaattca ggcttggagg atacgtatga
      541 ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa ggtgatgatt
      601 tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc
      661 atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca
      721 attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat
      781 acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca
      841 attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac
      901 ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt
      961 ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt
     1021 ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc
     1081 cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa
     1141 cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa
     1201 ttatgcaaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg
     1261 tagagcatgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca
     1321 ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt
     1381 tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg
     1441 aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg
     1501 ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcttgtttg
     1561 ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag
     1621 gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg
     1681 agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg
     1741 agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag
     1801 caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga ccgttgcaac
     1861 gattagtgac atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg
     1921 ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt gtcccataat
     1981 aggtaaaaag tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg
     2041 aaggaaataa aaaaattcat gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa
     2101 agaatgccat tattattgcg tctggttttg cccgcaggat ggatcatttt gctggtctgg
     2161 cggaatattt atcgcggaat ggatttcatg tgatccgcta tgattcgctt caccacgttg
     2221 gattgagttc agggacaatt gatgaattta caatgtctat aggaaagcag agcttgttag
     2281 cagtggttga ttggttaact acacgaaaaa taaataactt cggtatgttg gcttcaagct
     2341 tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg
     2401 cagtcggtgt tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca
     2461 gtctacccat taatgaattg ccgaataatc tagattttga aggccataaa ttgggtgctg
     2521 aagtctttgc gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata
     2581 acatgatgta tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca
     2641 agcaagatga agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt
     2701 ctttgttagg aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc
     2761 aatcggttac gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata
     2821 ttactgaacc gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa
     2881 ttgagattga aaatcaagca atttctctgt cttaaaatct attgagatat tctatcactc
     2941 aaatagcaat ataaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc
     3001 cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg
     3061 agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg
     3121 gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa
     3181 ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat
     3241 tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg
     3301 actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg
     3361 ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt
     3421 tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg
     3481 tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa
     3541 gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc
     3601 aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag
     3661 atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt
     3721 cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca
     3781 ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg
     3841 attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa
     3901 aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag
     3961 tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag
     4021 aaaaacaacg ttcgctatta tattagctaa ggagaaagaa atgaaatttg gattgttctt
     4081 ccttaacttc atcaattcaa caactgttca agaacaaagt atagttcgca tgcaggaaat
     4141 aacggagtat gttgataagt tgaattttga acagatttta gtgtatgaaa atcatttttc
     4201 agataatggt gttgtcggcg ctcctctgac tgtttctggt tttctgctcg gtttaacaga
     4261 gaaaattaaa attggttcat taaatcacat cattacaact catcatcctg tccgcatagc
     4321 ggaggaagct tgcttattgg atcagttaag tgaagggaga tttattttag ggtttagtga
     4381 ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg gttgaatatc aacagcaact
     4441 atttgaagag tgttatgaaa tcattaacga tgctttaaca acaggctatt gtaatccaga
     4501 taacgatttt tatagcttcc ctaaaatatc tgtaaatccc catgcttata cgccaggcgg
     4561 acctcggaaa tatgtaacag caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg
     4621 tattcctctc atctttaagt gggatgattc taatgatgtt agatatgaat atgctgaaag
     4681 atataaagcc gttgcggata aatatgacgt tgacctatca gagatagacc atcagttaat
     4741 gatattagtt aactataacg aagatagtaa taaagctaaa caagagacgc gtgcatttat
     4801 tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat
     4861 aattgcagaa aacgctgtcg gaaattatac ggagtgtata actgcggcta agttggcaat
     4921 tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg atttgatgag
     4981 ccaaaaaaat gtaatcaata ttgttgatga taatattaag aagtaccaca tggaatatac
     5041 ctaatagatt tcgagttgca gcgaggcggc aagtgaacga atccccagga gcatagataa
     5101 ctatgtgact ggggtgagtg aaagcagcca acaaagcagc agcttgaaag atgaagggta
     5161 taaaagagta tgacagcagt gctgccatac tttctaatat tatcttgagg agtaaaacag
     5221 gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga
     5281 ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac
     5341 ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc
     5401 aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat
     5461 cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta
     5521 ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga
     5581 gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa
     5641 tagaattagt caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg
     5701 ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag
     5761 attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc
     5821 ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaatct
     5881 cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa
     5941 aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg
     6001 atattagtca gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg
     6061 atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa
     6121 cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa
     6181 ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta
     6241 agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt
     6301 gtgctttaag cttaaccgaa gcgtttgata gttgatgacc tgcaggtcgg aagcataaag
     6361 tgtaaagcct ggggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     6421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6481 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     6541 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     6601 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     6661 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     6721 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     6781 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     6841 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     6901 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     6961 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     7021 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     7081 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     7141 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     7201 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     7261 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     7321 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     7381 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     7441 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     7501 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca
     7561 ccattcgatg gtgtcgggta ccgagctcca attcgcccta tagtgagtcg tattacgcgc
     7621 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7681 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7741 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
     7801 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccgt
     7861 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     7921 cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag
     7981 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg
     8041 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg
     8101 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc
     8161 tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt
     8221 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     8281 agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca
     8341 acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc
     8401 gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc
     8461 ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg
     8521 cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg
     8581 gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc
     8641 ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt
     8701 gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc
     8761 ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga
     8821 aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg
     8881 cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt
     8941 gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga
     9001 ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc
     9061 aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc
     9121 aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg
     9181 ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc
     9241 tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg
     9301 ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg
     9361 ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg
     9421 gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc
     9481 aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact
     9541 tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa
     9601 aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg
     9661 gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc
     9721 tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg
     9781 ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag
     9841 ggcgaagccc ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt
     9901 gcgaagcaaa gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt
     9961 gcgctgcccc cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg
    10021 cgatcatgcg atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg
    10081 cctaccgcga gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact
    10141 gggcggccag cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc
    10201 ggcgcaagga cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga
    10261 agtcggccag ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg
    10321 acaagtacgg ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc
    10381 acatgaccgc gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca
    10441 tcggcaacaa agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc
    10501 tagggctgca acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct
    10561 acgaggccct ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc
    10621 cacgcgccta tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga
    10681 cgccggaagc cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc
    10741 tacaggccgc cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg
    10801 cccgagacct tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg
    10861 atatgcaggc caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac
    10921 agcgggaagt ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt
    10981 tccccgaaaa gtgccacctg gattttggtc atgagattat caaaaaggat cttcacctag
    11041 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11101 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11161 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11221 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11281 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11341 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11401 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11461 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11521 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11581 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11641 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11701 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11761 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11821 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11881 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11941 agggcgacac ggaaatgttg aatactcat
//
