LOCUS       sequence-337558-       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histidine tagged NR1 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_His6_NR1.
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2389..2836
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2857..2877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2881..2901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2971..2992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3232..3251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3652..3708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3784..3801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6621..7209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6674..6694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7092..7103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7212..7228)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7213..7229)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7734..7914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7940..7957)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7946..8170
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8216..8644
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8303..8322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8513..8534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8653..8673)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8658..8987
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8838..8973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8900..8919
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9035..9082
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9101..9475
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9605..9726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9642..9661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9696..9715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9775..9791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9775..9791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9788..9810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9799..9815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9823..9829,9830..9847,9848..9853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9868..9889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10006..10023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10177..10765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10257..10276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10936..11727,11728..11796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11559..11578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11797..11901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11976..11995)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gagagggccc tgcgtatgag tgcaagtggg
     2401 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     2461 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     2521 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     2581 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     2641 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     2701 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     2761 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     2821 gtgcctgaga gcgcagtcgg atccgccacc atggctccaa aaaagaagag aaaggtagat
     2881 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatggt gagcaagggc
     2941 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3001 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3061 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3121 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3181 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3241 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3301 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3361 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3421 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3541 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg agctactaac
     3661 ttcagcctgc tgaagcaagc aggagatgtt gaagaaaacc ccgggcctgc tagcatgagc
     3721 accatgcacc tgctgacatt cgccctgctt ttttcctgct ccttcgcccg cgccgccact
     3781 aggcatcatc accatcacca cgctagttgc gaccccaaga tcgtcaacat cggcgcggtg
     3841 ctgagcacgc gcaagcatga acagatgttc cgcgaggcag taaaccaggc caataagcga
     3901 cacggctctt ggaagataca gctcaacgcc acttctgtca cccacaagcc caacgccata
     3961 cagatggccc tgtcagtgtg tgaggacctc atctctagcc aggtctacgc tatcctagtt
     4021 agccacccgc ctactcccaa cgaccacttc actcccaccc ctgtctccta cacagctggc
     4081 ttctacagaa tccctgtcct gggactgact acccgaatgt ccatctactc tgacaagagt
     4141 atccacctga gtttccttcg cacggtgccg ccctactccc accagtccag cgtctggttt
     4201 gagatgatgc gagtctacaa ctggaaccac atcatcctgc tggtcagcga cgaccacgag
     4261 ggacgggcag cgcagaagcg cttggagacg ttgctggagg aacgggagtc caaggcagag
     4321 aaggtgctgc agtttgaccc aggaaccaag aatgtgacgg ctctgctgat ggaggcccgg
     4381 gaactggagg cccgggtcat catcctttct gcaagcgagg acgacgctgc cacagtgtac
     4441 cgcgcagccg caatgctgaa catgacgggc tctgggtacg tgtggctggt cggggaacgc
     4501 gagatctctg ggaacgccct gcgctacgct cctgatggca tcatcggact tcagctcatc
     4561 aatggcaaga atgagtcagc ccacatcagt gacgccgtgg gcgtggtggc acaggcagtt
     4621 cacgaactcc tagagaagga gaatatcact gacccaccgc ggggttgcgt gggcaacacc
     4681 aacatctgga agacaggacc attgttcaag agggtgctga tgtcttctaa gtatgcggac
     4741 ggagtgactg gccgtgtgga attcaatgag gatggggacc ggaagtttgc caactatagt
     4801 atcatgaacc tgcagaaccg caagctggtg caagtgggca tctacaatgg tacccatgtc
     4861 atcccaaatg acaggaagat catctggcca ggaggagaga cagagaaacc tcgaggatac
     4921 cagatgtcca ccagactaaa gatagtgaca atccaccaag agcccttcgt gtacgtcaag
     4981 cccacaatga gtgatgggac atgcaaagag gagttcacag tcaatggtga cccagtgaag
     5041 aaggtgatct gtacggggcc taatgacacg tccccaggca gcccacgcca cacagtgccc
     5101 cagtgctgct atggcttctg catagacctg ctcatcaagc tggcgcggac catgaatttt
     5161 acctatgagg tgcacctggt ggcagatggc aagtttggca cacaggagcg ggtaaacaac
     5221 agcaacaaaa aggagtggaa cggaatgatg ggcgagctac tcagtggcca agcggacatg
     5281 attgtggcac cactgaccat caacaatgag cgtgcgcagt acatagagtt ctccaagccc
     5341 ttcaagtacc agggcctgac cattttggtc aagaaggaga ttcccaggag cacactggac
     5401 tcatttatgc agccttttca gagcacactg tggttgctag taggactgtc agttcatgtg
     5461 gtggctgtga tgctgtacct gctggaccgc ttcagtccct ttggccgatt caaggtgaac
     5521 agtgaggagg aggaggaaga tgcactgacc ctgtcctctg ccatgtggtt ttcctggggc
     5581 gtcctgctca actccggcat tggggaaggt gccccccgga gtttctctgc acgtatccta
     5641 ggcatggtgt gggctggttt cgccatgatc atagtggctt cctacactgc caacttggca
     5701 gctttcctgg tgctggatcg gcctgaggag cgcatcacgg gcatcaatga ccccaggctc
     5761 agaaacccct cagacaagtt catctacgca actgtaaagc agagctccgt ggacatctac
     5821 ttccggaggc aggtggagtt gagtaccatg taccggcaca tggaaaaaca caattacgag
     5881 agcgcagctg aggccatcca ggctgtgcgg gacaacaagc tgcacgcctt tatctgggac
     5941 tcggccgtgc tggagtttga ggcttcacag aagtgcgatc tggtgaccac gggtgagctg
     6001 ttcttccgct caggctttgg catcggcatg cgcaaggaca gcccctggaa gcagaacgtt
     6061 tccctgtcca tactcaagtc ccatgagaat ggcttcatgg aagatctgga taagacatgg
     6121 gttcggtatc aggaatgcga ctcccgcagc aatgctcctg caaccctcac ttttgagaac
     6181 atggcagggg tcttcatgct ggtggctgga ggcatcgtag ctgggatttt cctcattttc
     6241 attgagatcg cctacaagcg acacaaggat gcccgtagga agcagatgca gctggctttt
     6301 gcagccgtga acgtgtggag gaagaacctg caggatagaa agagtggtag agcagagccc
     6361 gaccctaaaa agaaagccac atttagggct atcacctcca ccctggcctc cagcttcaag
     6421 agacgtaggt cctccaaaga cacgagcacc gggggtggac gcggcgcttt gcaaaaccaa
     6481 aaagacacag tgctgccgcg acgcgctatt gagagggagg agggccagct gcagctgtgt
     6541 tcccgtcata gggagagctg aggcgcgcct tcgaattaat taaggccgcg actctagaat
     6601 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6781 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6841 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6901 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6961 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7021 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7081 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7141 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7201 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     7261 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7321 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7381 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7441 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     7501 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     7561 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     7621 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     7681 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     7921 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7981 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8041 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8101 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8161 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8581 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8641 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8701 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8761 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8821 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8881 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8941 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9001 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     9061 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     9121 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     9181 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     9241 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     9301 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     9361 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     9421 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     9481 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     9541 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9601 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9661 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9721 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11941 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    12001 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    12061 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    12121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
