LOCUS       sequence-337562-       11461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the purifcation of human ULK1 complex .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, 
            Hurley JH
  TITLE     Reconstitution of cargo-induced LC3 lipidation in mammalian 
            selective autophagy.
  JOURNAL   Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 
            10.1126/sciadv.abg4922. Print 2021 Apr.
  PUBMED    33893090
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-04-23"; volume: "7(17). pii: 7/17/eabg4922. doi"; pages:
            " 10.1126/sciadv.abg4922. Print 2021 Apr"
COMMENT     Sequence Label: GST-TEVcs-FIP200-MBP
FEATURES             Location/Qualifiers
     source          1..11461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1754..1763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1760..2416
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2374..2396
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2444..2464
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7265..8365
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     8339..8362
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     primer_bind     complement(8483..8502)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8548..8603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8602..8621)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8964..8980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8964..8980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8977..8999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8988..9004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9012..9018,9019..9036,9037..9042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9057..9078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9137..9332
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9183..9318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9338..9472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9375..9394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9429..9448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9540..9557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9711..10299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9791..9810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10470..11261,11262..11330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11093..11112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11331..11435)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gcgccrggsg gggsggggsg gggsgrgggg
      541 sggggsgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgat
     1741 cgacggtatc gatgccacca tggcatcccc tatactaggt tattggaaaa ttaagggcct
     1801 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
     1861 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
     1921 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
     1981 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
     2041 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
     2101 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
     2161 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
     2221 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
     2281 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     2341 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     2401 cgaccatcct ccaaaatcgg atgattacga tatcccaacg accgaaaacc tgtattttca
     2461 gggtggtacc atgaagctct atgtgtttct cgtgaatacc ggcactaccc tgacctttga
     2521 taccgaactg accgtccaga ccgtggctga cctgaagcat gctatccagt ctaaatacaa
     2581 gatcgcaatt cagcaccagg tgctggtggt caacggcgga gagtgcatgg ccgctgatcg
     2641 cagggtctgt acctattcag ccggcaccga cactaaccca atcttcctct ttaataagga
     2701 gatgattctg tgcgatagac cccctgctat ccccaagacc acttttagca ctgagaacga
     2761 catggaaatc aaagtggagg aatccctgat gatgccagcc gtgttccaca ccgtcgcttc
     2821 tcgcactcag ctggcactcg agatgtacga agtggccaag aaactgtgct ctttctgtga
     2881 gggtctcgtc cacgatgaac atctgcagca ccagggctgg gcagccatta tggctaacct
     2941 ggaggactgt tcaaatagtt atcagaaact gctcttcaag tttgagtcta tctactcaaa
     3001 ctatctgcag agcatcgaag acattaaact gaagctcaca cacctgggaa ccgcagtgag
     3061 cgtcatggcc aagatccctc tgctcgagtg cctgactagg cattcctaca gagaatgtct
     3121 gggtaggctc gatagtctgc cagaacacga ggacagcgag aaagccgaaa tgaagcgctc
     3181 tacagagctg gtgctctcac ctgacatgcc aaggacaacc aacgaaagtc tgctcaccag
     3241 cttcccaaag tccgtggagc atgtctcccc cgacactgcc gatgctgaat ctggaaagga
     3301 gattagagaa agttgccaga gcaccgtgca ccagcaggac gagactacaa tcgatactaa
     3361 ggacggcgat ctgcccttct ttaacgtcag tctgctcgat tggatcaacg tccaggacag
     3421 acctaatgat gtggagtccc tggtccggaa gtgctttgat agcatgtccc gtctggaccc
     3481 acgtatcatt cgtcctttca tcgcagagtg tcgtcagacc attgccaagc tggacaacca
     3541 gaatatgaaa gctattaagg gtctggaaga tcggctctac gccctggacc agatgatcgc
     3601 tagctgtgga cgcctggtga acgagcagaa ggaactcgct cagggtttcc tggcaaacca
     3661 gaaaagagcc gagaatctca aggatgcatc agtgctgcct gacctgtgcc tcagtcatgc
     3721 caatcagctg atgattatgc tccagaacca ccgcaagctg ctcgacatca aacagaagtg
     3781 taccactgct aaacaggagc tggcaaacaa tctccatgtg aggctgaagt ggtgctgttt
     3841 cgtcatgctg cacgcagacc aggatggcga gaagctccag gccctgctca ggctggtgat
     3901 tgagctgctc gaaagagtga agatcgtcga ggctctgtcc accgtcccac agatgtactg
     3961 cctggcagtg gtcgaggtgg tcagacgtaa aatgtttatc aagcactaca gagaatgggc
     4021 aggtgccctc gtgaaagatg gcaagcgtct gtatgaagcc gagaaatcta agcgtgagtc
     4081 attcggcaaa ctgtttcgga agagcttcct ccgtaaccgg ctgttccgcg gactcgacag
     4141 ttggccaccc agcttttgca cccagaaacc taggaagttc gactgtgagc tgccagatat
     4201 ctccctcaag gacctgcagt ttctccagtc cttctgccca tctgaggtgc agccctttct
     4261 gagggtccct ctgctctgtg acttcgaacc tctgcaccag catgtgctgg ccctccacaa
     4321 cctcgtcaag gctgcacaga gtctggatga gatgagccag acaatcaccg acctgctcag
     4381 cgaacagaag gcttctgtgt cacagacatc cccacagtct gctagctccc cccgtatgga
     4441 gagcaccgca ggcatcacaa ccactacatc cccacgtaca cctccaccac tcaccgtgca
     4501 ggatcccctg tgccctgctg tctgtcctct ggaggaactc tctccagaca gtattgatgc
     4561 acacactttc gactttgaga caatccctca tccaaacatc gaacagacca ttcaccaggt
     4621 gtccctggac ctcgattctc tggccgagag ccccgaatcc gattttatgt ccgctgtgaa
     4681 tgagttcgtc attgaggaaa acctgtcttc acccaatcct atctcagacc cacagagtcc
     4741 cgagatgatg gtggaatccc tgtacagtag cgtcatcaac gccattgatt ctcggcgcat
     4801 gcaggacaca aatgtgtgcg gcaaggagga ctttggagat catacctctc tgaacgtcca
     4861 gctcgaacgc tgtagggtgg tcgctcagga ttctcatttc tcaatccaga ctattaagga
     4921 ggacctgtgc cacttccgca catttgtgca gaaggaacag tgcgacttca gtaacagcct
     4981 gaaatgtaca gccgtggaga tcaggaacat catcgaaaag gtcaagtgtt ctctggagat
     5041 caccctcaaa gaaaagcacc agaaagagct gctctcactg aagaacgaat acgagggaaa
     5101 actggacggt ctcattaagg aaaccgagga aaacgagaat aaaatcaaga aactgaaggg
     5161 agagctggtg tgcctcgagg aagtcctgca gaacaaggat aatgaattcg ccctggtgaa
     5221 acatgaaaag gaggctgtca tctgcctgca gaatgagaaa gaccagaagc tgctcgaaat
     5281 ggagaacatt atgcactcac agaattgtga gatcaaagaa ctgaagcaga gtagagagat
     5341 cgtgctggaa gatctcaaga aactgcatgt cgagaatgac gaaaagctgc agctgctccg
     5401 tgccgaactc cagagcctgg agcagtccca cctcaaggaa ctggaggata ctctgcaggt
     5461 gcgccatatc caggaattcg agaaggtgat gacagaccac agagtcagcc tcgaggaact
     5521 gaagaaagag aaccagcaga tcattaatca gatccaggag tcccacgctg aaatcattca
     5581 ggaaaaagag aagcagctgc aggagctgaa actcaaggtg tctgacctca gcgatacccg
     5641 ctgcaaactg gaagtcgagc tggccctcaa ggaagctgag actgacgaga tcaaaattct
     5701 gctcgaggaa tctcgggccc agcagaaaga aaccctgaag tcactgctcg aacaggagac
     5761 cgaaaatctc cgtactgaga tcagcaaact gaaccagaag attcaggata acaatgagaa
     5821 ttaccaagtg ggactcgctg aactgagaac tctcatgaca atcgagaagg accagtgtat
     5881 ctctgaactg atttcacgtc atgaggaaga gtcaaacatt ctgaaagcag agctcaataa
     5941 agtgactagt ctgcacaacc aggccttcga gatcgagaag aacctgaagg agcagatcat
     6001 tgaactgcag tccaagctcg attccgaact gtctgccctc gagaggcaga aagacgaaaa
     6061 gatcacacag caggaagaga agtatgaggc tatcattcag aacctcgaaa aagataggca
     6121 gaagctggtg tcctctcagg agcaggacag agaacagctg attcagaaac tcaattgcga
     6181 gaaggacgaa gcaatccaga cagccctcaa agagttcaag ctggagagag aagtggtcga
     6241 gaaagaactg ctcgagaaag tgaagcacct ggaaaaccag atcgctaagt cacccgcaat
     6301 tgatagtacc cgtggtgact caagtagcct ggtggccgag ctccaggaaa agctgcagga
     6361 agagaaggct aagtttctgg agcagctcga agagcaggaa aaaagaaaga acgaagagat
     6421 gcagaatgtg cgtacaagcc tgatcgccga gcagcagacc aacttcaata ccgtcctgac
     6481 tcgtgagaaa atgcggaagg aaaacatcat taatgacctc agcgataaac tgaagtccac
     6541 catgcagcag caggagcgcg acaaggatct catcgagtcc ctgtctgaag acagagctcg
     6601 tctgctcgaa gagaagaaaa agctggaaga ggaagtcagt aagctgaggt cctcttcatt
     6661 cgtgccaagc ccctacgtcg ctactgcacc cgagctgtat ggagcctgcg ctcctgagct
     6721 gcctggtgaa tcagaccgga gtgccgtgga gactgctgat gaaggccgcg tcgactctgc
     6781 catggagaca agcatgatgt ccgtgcagga aaacattcat atgctgtcag aggaaaagca
     6841 gagaatcatg ctgctcgagc gtaccctgca gctcaaagag gaagagaaca agcgcctcaa
     6901 tcagaggctg atgtcacaga gtatgagtag cgtgtcctct cgccactccg agaagatcgc
     6961 cattagggat ttccaagtgg gagacctggt cctcatcatt ctggacgaac gccatgataa
     7021 ctacgtgctg tttactgtct ctcctacact gtatttcctc cacagcgagt ccctgcccgc
     7081 tctggacctc aagcctggtg aaggcgcatc tggcgcctca aggagaccct gggtgctggg
     7141 aaaagtcatg gagaaggaat actgccaggc taaaaaggca cagaaccgtt ttaaggtgcc
     7201 tctgggcacc aaattctatc gggtgaaggc cgtctcctgg aataaaaagg tccccggggc
     7261 tagcatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
     7321 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
     7381 gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga
     7441 cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga
     7501 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
     7561 ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa
     7621 caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
     7681 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
     7741 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat
     7801 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
     7861 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
     7921 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
     7981 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt
     8041 cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga
     8101 gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc
     8161 gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc
     8221 cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc
     8281 tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga
     8341 tgaagccctg aaagacgcgc agactaatcc cgggtgatat cttaattaat taactcgagg
     8401 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     8461 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     8521 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     8581 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     8641 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     8701 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     8761 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     8821 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     8881 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     8941 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9001 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9061 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9121 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9241 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9301 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     9361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     9481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11461 g
//
