LOCUS       sequence-337566-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  H2B-sfGFP with a synthetic intron from the pCI expression vector, 
            T2A, and tdTomato with synthetic introns derived from the rabbit 
            beta-globin gene and the human beta-globin gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Fluorescent protein with synthetic intron plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI
            syntron-T2A-t...
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        2406..2785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2787..3064
                     /label=chicken beta-actin promoter
     intron          3065..4081
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4005..4027
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4089..4108
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4141..4174)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4307..4428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4540..4661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4773..4894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4917..4950)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4972..5349
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     primer_bind     complement(5398..5426)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          5650..5782
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6154..6174
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6236..6289
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7989..8009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8407..8418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8563..8580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8569..8793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8990..9009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8991..9009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9016..9033)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9016..9032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9025..9047)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9174..9629)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9311..9332)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9523..9542
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaacaagggc aggatgtgtc aaactgccaa tagagaacta
     2221 cttactcttc aggctgaagc tgatggaaca ggtaacaaag gcaaacacta atcatgatca
     2281 gcaagatgaa gcagaaaggg aacaagggga tattaaatgt gtatagacac gctagagaga
     2341 tggctcagca gttaagagaa ctagctggtc tttcagaggt cctgagatca ataccggtca
     2401 tggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2461 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2521 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2581 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2641 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2701 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2761 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2821 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     2881 agcgatgggg gcgggggggg ggggggggcg cgcgccgggg ggggsggggs ggggsgrggg
     2941 gsggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3001 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3061 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3121 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3181 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3241 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3301 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3361 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3421 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3481 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3541 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3601 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3661 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     3721 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     3781 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     3841 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     3901 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     3961 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     4021 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     4081 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgctagc
     4141 ataacttcgt atagcataca ttatacgaag ttatagccac cgcggtggcg gccgctctag
     4201 aatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     4261 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     4321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac tagaatcata
     4441 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4501 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4561 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4621 cattctagtt gtggtttgtc caaactcatc aatgtatctt aactagaatc ataatcagcc
     4681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4861 gttgtggttt gtccaaactc atcaatgtat cttaactagt ggatctcatg cctaggataa
     4921 cttcgtatag catacattat acgaagttat aggcgcgccg gtaccgccac catgcctgaa
     4981 ccctctaagt ctgctccagc ccctaaaaag ggttctaaga aggctatcac taaggcgcag
     5041 aagaaggatg gtaagaagcg taagcgcagc cgcaaggaga gctattctat ctatgtgtac
     5101 aaggttctga agcaggtcca ccccgacacc ggcatctcat ccaaggccat ggggatcatg
     5161 aactccttcg tcaacgacat cttcgagcgc atcgcgggcg aggcttctcg cctggctcac
     5221 tacaataagc gctcgaccat cacctccagg gagattcaga cggctgtgcg cctgctgctg
     5281 cctggggagc tggctaagca tgctgtgtcc gagggcacta aggcagttac caagtacact
     5341 agctctaagg atccaccggt cgccaccatg agcaaaggag aagaactttt cactggagtt
     5401 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
     5461 gagggagagg gagatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
     5521 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
     5581 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
     5641 tatgtacagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
     5701 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
     5761 cactttgcct ttctctccac aggaacgcac tatatctttc aaagatgacg ggacctacaa
     5821 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
     5881 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc
     5941 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     6001 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
     6061 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
     6121 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     6181 tgggattaca catggcatgg atgagctcta caaaggcggc tccggcggcc gctccgaggg
     6241 ccgcggcagc ctgctgacat gcggcgacgt ggaagagaac ccgggccctg gcagcacaat
     6301 ggtgagcaag ggcgaggagg tcatcaaaga attcatgcgc ttcaaggtgc gcatggaggg
     6361 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6421 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     6481 cctgtccccc caattcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     6541 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6601 ggacggcggt ctggtgaccg tgacccaggt aagtttgggg acccttgatt gttctttctt
     6661 tttcgctact ctgattccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt
     6721 ttccttcagg actcctccct gcaagacggc acgctgatct acaaggtgaa gatgcgcggc
     6781 accaacttcc cccccgacgg ccccgtaatg caaaagaaga ccatgggctg ggaggcctcc
     6841 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggctctgaag
     6901 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     6961 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgaa
     7021 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
     7081 catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
     7141 atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     7201 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     7261 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccaa
     7321 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     7381 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     7441 gtgaccgtga cccaggtaag tatatgggac gcttgatgtt ttctttcccc ttcattctga
     7501 gtccaagcta ggcccttttg ctaatcatgt tcatacctct tatctttttc cttcaggatt
     7561 cctccctgca agacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     7621 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     7681 acccccgcga cggcgtgctg aagggcgaga tccaccaagc cctgaagctg aaggacggcg
     7741 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     7801 gctactacta cgtggacacc aagctggaca tcacctccca caacgaagac tacaccatcg
     7861 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
     7921 tgtacaagta aggcgcgcca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8041 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8101 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8161 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8221 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8281 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8341 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8401 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8461 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8521 ccgcaggatc cactagagcg gccgcctaga gctcgctatc agcctcgact gtgccttcta
     8581 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8641 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8701 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8761 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8821 gctcgactag agcttgcgga acccttaagc ttgtcattcg aatttagaca cccacatggt
     8881 ggctcatgac catctatcta taaatggatc tgattttcat gtctggcagt gtacagaagc
     8941 taactgaaga aaggtggaag acccacaaga gttcaaggta cccaattcgc cctatagtga
     9001 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9061 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9121 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt
     9181 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     9241 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     9301 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     9361 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9421 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9481 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9541 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9601 cgcgcttaat gcgccgctac agggcgcgtc ag
//
