LOCUS       sequence-337594-       13848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CHD7 (K999R) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7 (K999R)
FEATURES             Location/Qualifiers
     source          1..13848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             97..630
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  697..921
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(1055..1072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1226..1814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1306..1325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1985..2776,2777..2845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2608..2627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2846..2950)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2976..3430)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3113..3134)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3324..3343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  3614..3779
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    3808..3942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3832..3851)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3886..3905
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4130..4153)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      12794..12803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(13145..13165)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(13181..13198)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(13285..13376)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(13343..13360)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(13728..13733,13734..13750,13751..13756)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
       61 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt
      121 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc
      181 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt
      241 cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg
      301 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt
      361 ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc
      421 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat
      481 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac
      541 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac
      601 tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat cggcttcccg
      661 gccgcggagt tgttcggtaa attgtcacaa cgccgcgaat atagtcttta ccatgccctt
      721 ggccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt
      781 tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat ttcagtttac
      841 aactattctg tctagtttaa gactttattg tcatagttta gatctatttt gttcagttta
      901 agactttatt gtccgcccac acccgcttac gcagggcatc catttattac tcaaccgtaa
      961 ccgattttgc caggttacgc ggctggtcta tgcggtgtga aataccgcac agatgcgtaa
     1021 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2941 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     3001 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3061 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3121 gaacaagagt ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3181 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3241 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3301 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3361 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3421 cagggcgcgt cccattcgcc attcaggctg caaataagcg ttgatattca gtcaattaca
     3481 aacattaata acgaagagat gacagaaaaa ttttcattct gtgacagaga aaaagtagcc
     3541 gaagatgacg gtttgtcaca tggagttggc aggatgtttg attaaaaaca taacaggaag
     3601 aaaaatgccc cgctgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt
     3661 ttttaaggat tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa
     3721 gctaatacga aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttc
     3781 ggatctccta ggctcaagca gtgatcagat ccagacatga taagatacat tgatgagttt
     3841 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3901 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3961 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4021 tacaaatgtg gtatggctga ttatgatcct ctagtacttc tcgacaagct tggtaccgca
     4081 tgcctcgaga ctgcaggctc tagattcgaa agcggccgcg actagtctac ttgtcatcgt
     4141 cgtccttgta gtcttcatca ttttcattgt tttctatttc atccccccca tcaagtgagt
     4201 ctagctcttc accctgtgct atttcgtctt ctaagaggga ggactctgca gtcttatcca
     4261 ggctctcctc ggcatcgctg ccttcaaggg tctctccatc tttaaagggt cctccctcag
     4321 ccttgtcagc agccttttct tcgctggagc ccacggcatt ctgaagtcct gccaatgctg
     4381 ggaacccagg cagcgtcaat ccccccagtc ctggaggtag aaacatggat gggtagaaga
     4441 ggcccggggc cattgtggac aggaggaaag ggttgaaggc tagcgggttt gagggcaatc
     4501 cagccggggc agtagcagca ccaacagatc cattcgcaga gtcagcagcc gaaacagcat
     4561 ctgtgctttt ctcagagtct ttgttctcgt cttcattctc atttcctttc tcctctcctt
     4621 ttgaagtgct gtcttccggt tctccttgac tagaagcagt agtggtgttt ccagtagcag
     4681 ctgacagagg gttattcaac agcccgccca agccaaacac gttgggcagg cccgccattc
     4741 ctggcagcat caggggcagc acagcagcag ggttcttcgc atcgcctccg gcggtggcag
     4801 ctgttgccag tcctggaggg aagcccatga ggcctgccag ctggagcgac tggagattct
     4861 ggagattctg aaggctcgtc aggtccattc cagcaaacag gctgttcacc agcaaagggt
     4921 tgatccctga cgtggaggcc acagcagcgg cggccgcggc tgctctggcg atctcacttt
     4981 tgggccttct tcctcttctg ctcgctccct ctccccgcac tacaggccca gtgagaaggc
     5041 ggtcaaacat cgactcagga acaaaaccag actgcttaac tatatcagtc cagtctggag
     5101 caactgcaaa ttcaggattt tcttccagcc acctgggtag atccttcatt ggaggcgcca
     5161 tagctccacc catcttcttc ccatttcgtt tattgacaac aggcaccctt tcttctcctg
     5221 tcaaagtgtt tatatccaat ttattagggt ttcgacatct atgtcgtttc tgtttcggtt
     5281 tctgaaatga ggaaaacagc acatctgcat tctttggtac ataacttggc atatcaacag
     5341 tgtaagtagg gtgcagcttc agccattcaa ctaaatcctt atttttagga gcatcttccc
     5401 ccaccagcct agtcccatct tcaagattga taacagggat ccgtgtgtct gggtccagct
     5461 gtccgggaga aggaatgttt cttgttggtg gggtctctat atcttcttca aaagctttgg
     5521 tcacctcagc atcctctgca ctcaatgacg tccgtttgtg gctcatgaaa agcagatcaa
     5581 gtccctccac atttttcctc cttccccgcc tcctcttcat gggaggctct ccatccacta
     5641 gggagccatt aaggagatgc cttgtgggtg tgcgcgaaag gcctgcttgg agcagttcca
     5701 tttgagtctt aaaggcatca gacactggtg ttgagacatt aggcaagata aactttgaag
     5761 aaagagatga aaaatttgag gtagagcttg ttgcctctct tgaggccttt gataaatcta
     5821 caactttttc ttgtccattt tctgagacca cctgagactc tcgaagctgg gcaaccatct
     5881 ccatgagatt tcgcctcttg gcagctcttt cggcctcaat ttcgattttt ctcctcctcc
     5941 tcctccgctg gcgagggaca gagaggttga gggcatcttc ctttgacaac tgaggagacg
     6001 tagtgatgtc ctcactccca ctaatgctgg cttggccgac catggagagc tcagcaaagc
     6061 tcctcttctg caaggggctg tccactgtgg tgggtgtgta acctgggatg aggccttgga
     6121 aatcaaacat ctggcgccta tttactggcc atttgccttt caacactgct tcacagatgt
     6181 tgtctaagcg gtttatcatt actctatcct taggccaaaa cgagaaggca aatgttcttt
     6241 catgaaggag ctgagctact gaaggatctc cgtcctccat gtagaatcca tctcgggttt
     6301 catctctagc agtgctcatg gaagcattgc tttcttcatc aaaattcttc cctctagaga
     6361 cagctcctgc ttcgggctgg gaagactctt ccgacttatc gtcatcctcc agcttttcct
     6421 cttcatcctc ttcggaacct ttctctgaaa tagatttgga cccagtgtct gcgccgacct
     6481 caacaccttt cagttcattt ttcacagagc tggcctctgc ctcacattcc tgcttgctct
     6541 ccttcccgct gccatcggtt tcttcttcct cttctttgcc ctcacatttc tccttggcag
     6601 ctgggttttc aggctcctcc actttgcctt cggcttgttc cagtaccctc tcatcttgaa
     6661 tatgagcaga tgagatgact ggaggagtct ggacaaatcc aactgccagt gggttcaagg
     6721 aagatgtatt acctgcccct ctgttttgag caaagttttt atgtgcatcc aagaaggata
     6781 actcagggtc attgaggatg tgataatccg tccgactgac cccgtgttta gcagcaccaa
     6841 ccagcaagtc tcggtcatgc cgtccacact cccaccactc tggcagatcc aagcttggct
     6901 ggcagagctt aagcctctct cccagctggg ggtgatggag aacctgctcg cggatcttcc
     6961 gtagcagctc aatgcggtac agagttcgag aggctcgctc ctctgtgatc ggctcaatta
     7021 tggaggagag gtcgggcggt tcatcatctg gcttgacggg cattcgacat actcgcctac
     7081 acatggccac aaaacaactg aagtatttct ccaaactctc atcagatttt ttgtcaagcc
     7141 tggcaaaggc tctaaattgg ttccagtcaa attgctgttt cacagggtca aaaataaccc
     7201 caaaggtgga taccacacgg taaaaatcag cctcttctct tcttgtccac ttttgccgct
     7261 tctcagatat aatagcttcc ctttccgctt ccagagctct cacttcctct cgaggccgcc
     7321 gtctgcgccg gtcagtcttc attagggcct cttgcctcat ctgttgcctt ttatagctgc
     7381 gctgataggc agtaatgagc cggcgcagac gtgtagtcag ggttgaagtg ttaggccagt
     7441 aaagttggcc taactcagca ttactctcac tgtgcttgcc tgtggcatgt atttccatgg
     7501 attcttcctt atcctctgaa ggagaatttg caaattcatc tatttcatct ttgaacggtg
     7561 ttctggttgg tttatattct gggtcttcat cttctctatc aaattctccc ccgtcaccac
     7621 catctgctag catgtctgtt cctctttgct cggcagctat ggccttggca tcaggcatac
     7681 cgactcgttc cagaaagcac agcgcggggt cagctcgcat ggagttgtac ttctcatagc
     7741 catgtttgaa cactccaatt aagagggatt tgtctgcttc cttatcccac caatctgcag
     7801 gaacttcagc atggaaaggt tcagggatcc acacatcggc ttcacttgag tcagcaccct
     7861 ctaagatctt atccgcctgg tctcctatca cttcttgtct taggtagtac agcatgcgga
     7921 cacgcagcag gaccttgtta cagtgatgct tcaggtgttt cttgtagctg tcctcctgga
     7981 acagggcatc tgggttgcag ctggccagcc agtcggcatc ctgcaccacc ggctgtgtgc
     8041 tctgggcttt caccttcttt cccttccttc cccttggcac aggagctgac aaaccggaat
     8101 ggttgaccaa ggctcgagtc tggccatccg ctgtgggtgt gatcagatcc cagatgaagc
     8161 ttttgatatt ctcatcccct ttgtaatgat taagacagta caccaggatg gttctgcaga
     8221 tggtttctac atcttgctca gtgagttggc gtttatagcg tccgtgggaa agaatgtctg
     8281 tccaccgtcc ccaaccatag acaagcagat tcttctccac cctgaaacat tcactccttg
     8341 catagccctg tgacttatcc tggggacgcc gtggctttgc acagggcttt tcttcagaat
     8401 cactttccaa gtctgagaac tccatcagct catcttcctt cactgcactg tagagcctgg
     8461 tctgctttct cactcttgga gtatcaataa ccaggttgtt cctcccattt aaggcatcaa
     8521 tatccaattc agccttctta gcccactttt gccagaaatt tggatcatcc aaggaaatat
     8581 ctgtcctatt tccagatgca acaaaactgg ccttagcaaa tgtggaacct ttcccttctg
     8641 actcaatggt aatggtgtgg gttcgacgta ggaggatctg atcaatatct tcttcacaga
     8701 atttagaccc ttcatcctcc tcatccatga gtgcaccata ggcccctttt cgtagaagat
     8761 cctctatttc tttcttggaa agctgttgta ccccattggt agcattttct cttccactca
     8821 tagactgtag cacagcttta tccaggccca gtttcaaact agccttgtcg aacatttccc
     8881 tttcatagga atttcttgta atcagcctgt agattttcac agatttgctc tgtcctattc
     8941 tatgacatct agcctgagcc tggaggtcat tttggggatt ccagtctgaa tcaaagatga
     9001 tgcaggtatc agcagcagtg aggttaatgc ctaaacctcc tgcccttgta cacaggagga
     9061 aaacaaacct atcagaatca ggtttggaga atctgtcgat agctgcctgg cggaggttgc
     9121 ctcttactcg gccgtcgatc ctttcatatg ggtaccgtct ttgaatgagg tagtcttcca
     9181 gtatgtccaa gcagcgcacc atctgggaaa agataagcac cctgtggcca ccagccttca
     9241 gttttggcag cagcttgtca atcagcacta gcttgccagc agcctggatc attgcctgga
     9301 gctgaaaatc tggagactct gcattgtgtg tttctttaaa ctcttccaaa attttctctt
     9361 cagcaccatt gataaggtac ggatgattgc agcacttccg caattccatc atagtgttta
     9421 ataggttagg tacgttagct tgaccaccgc ctttggaaag aaatgtgaaa ttcttctcaa
     9481 ggatggctcg gtaatatttc ttctgaatgt ttgttagctc aacttcaata atagtttctt
     9541 ctttgggggc caagttcttt tctacatcct ctttgagacg tctcaacatc attggcttta
     9601 gaatagcttg aagtttttgc acctgctctt ctgtttttag atcaccaaat tcttgcataa
     9661 atgtggtttc tgaagggaag cgacttggtt ccaagaaatg aagcaagctg aagagttctt
     9721 ccacagtgtt ctggagtggg gttcccgtca gcagcacttt gtgttccaag tccatcatct
     9781 tgagtccctc caacagcttg cagttcctgt tcttcagcct gtgggcttca tcaatgacta
     9841 cacagcgcca tggaatattc cgcagctcag gacaatcagt caaaatcatc tcaaatgtag
     9901 tgatgatggc atgaaactta taggacccct ttatcactcg accctgggga tctttgaagt
     9961 acatttcata caactgaatg gtccgacgac tagcttgact cccatgatac acaaccacgt
    10021 tcaactctgt ccaggttcgg aattcccttt cccagttggg gattgtggac aatggggcaa
    10081 ttactaaaaa agggccatgg attcctttca aatatatctc atagagaaat gtaatggact
    10141 ggatagttct tcccaaaccc atttcatctg ctaaaatgca gtttcgcatg ttgtaccaat
    10201 tgaaaagtag ccagtttact ccctccaact ggtattccct gagtttgtta ttgtttttat
    10261 actccctgga actctccgat ttcttccaat catcagcagg aggtcgctcc acacgctctg
    10321 tttccggctc cctggacatt agtttctcaa actcctcgat ctttgcttga tctatgtcct
    10381 gcctccgctc ccacgtgctg tcttcataag gaagtgaaca ccacttcacc agatagtgag
    10441 tcacaggctc tccccggtca tctgtgctac gtgcaaagtc cattatccgg tcaacctcca
    10501 cataatctgg attaaaaagc tcatcctcaa tctctgaaag gaacttgttc tggccctgct
    10561 ttgccttaaa tcgtttaatt ttttgctgaa ttctcttatc tttttccaga tcttctatag
    10621 atgcccactg acaatgaaga taagagaagt ttttgtattt cacatagaat tcctcaattt
    10681 ctacctcctc tccagattcc ttctgctttt ttactgaacg actgctcata attttttcta
    10741 ccactgggcc ttctgcatca acagattcct gctgttctga ctgggaggtg ttggaggggg
    10801 aatccctccc agcagcatct gcatcatctg cctcctcatc agaaatcttg aactccaggt
    10861 cttcagtgta gcgctttctc ttcacctgtc tgctggaccg tctcttctga acacctgggt
    10921 cctcatcttc ttcaggagga ggagatggtg ggggtgtttt gtctaagtct gaattttcag
    10981 aaccttctgt ttttcccttg ttgacctttt tcttcaaagc acttgcttct gagtcaggtt
    11041 tcttattact tgactttttg ctggatttgg gttttggcgt ggcagttttt gctttctttt
    11101 cctttggctc tttgggaatc ttaggggctt tcggggtctt gggttccttg ggctcttttt
    11161 tctccttggg ttctttcggt tccttcgggt ctttttttgc ctttgacctt ttctttttct
    11221 ttttttcttc ttgggaccca tccagtgagt tcctatttag ttcttggtta tctaccccac
    11281 cagggaagtc atctttacca aaacctttac tgggatcctc tgctacaatg tggttgtttt
    11341 tctttttctt cttcttttgt tgtggctgct gttctattga tggcaggtaa tcatcactct
    11401 tcactagctg agcattcggt ccactaacct gagtcatatc cggcactggt ttctctagaa
    11461 agggctccga cggggaatgc tgatgcacag gcactttctg cggggtgttc tggggtgatg
    11521 ggtggagctg tgcccaaggc tggtgatgag ggtgtggagg tgaagactgg tggtgcaagc
    11581 ccgggtgagg ctgcagtgga ggacaggttg gcaactgctg aaaagatggc tgttgaccag
    11641 gatgttgttg gccaggtatc agtcgttcct ggattgcttg tgggtctcca aggccaacac
    11701 caggacaacc atttggcctc atgtgcccag tcaattccct tggtgccgag gacatgccta
    11761 taaatggacg agactgctgc atgtttctgg ggcccatatt cctctgtccg attcccatgg
    11821 caccaggggg ctggtgagat ggctgaggat ggggcatatt tggataactg ccaacttcca
    11881 ttggtatccc agcacttccc ggcctgactt gtggaggagg agtgcctgct ggattactca
    11941 ttgctttcat gggtgacatg ggaggtggag aggcataagt tccctgaggc tgtgaaggat
    12001 gcatagtttg tgtgttcatt tggttaagtg agccactggg gtggatgggc tgctggtgca
    12061 ttagtccttg accactgttt gatgggaatc ctacagcatt ggggtatctt ggtacggact
    12121 gattcattgg agtattattt gtaaggccta aattttgatt catccctgta ttgttaacta
    12181 atccctgatt taggttactg taaggatatc gagaatactg ccctgagttg tttatagtag
    12241 gagaggggac tgtctggctc cgagaactaa agttaagggt ttgcggccta acagcccctt
    12301 gttggggagg attcggggag aatctgggac tgtgggcaac ggattctcca tggagagcag
    12361 tagaggggtg gtgatggaac tgctgcaccg agtgacgcaa ggaaggtgcc atgctgggac
    12421 tctgctgggg cacgtgggac aagtggccag gtcctgaggt ggcaataaaa ggatttccct
    12481 gattgaggcc ctcttggcct tgggaaaact ggctcatcct ctgctgtggc tgaccatgct
    12541 gctgcatgga aaaatcccca cgtgccatat agctgcccat ctgctgcatg tgctgagggt
    12601 ggccctgtgc agggggcccc gacggagccg gctgcggtgg ctgcggctgt ggctgctgct
    12661 gctggtaggg ggctcgtatc tggtctggta cctgaacagc cctggggccc cacatggagc
    12721 tgctgtccac aaaggattgc ccatgcctct cattctgcat gccagggtag acacccatct
    12781 gaccgccacc actgccacca tggggcacct gaggaacggg aggggtgtga tactgcgagt
    12841 gcggagacgc gagtccgttc ccaggggtgt tgctcatcat tctgttcggc tgatccatca
    12901 gatgcatctt ttgttgttca tactgattat agtgatcaaa atgtgtcagc tttgtttgat
    12961 tttgattagt tgaaggatga tgaagggatg gctgtaaaga ggcaaagcct tggtctattg
    13021 gcatttgctg acccatagga tttactggat tttccgggta accacattct ccgaggcctt
    13081 caagaccttc actgaaaata ttcccatcct cgccaaaaag actcatcatt cctggatctg
    13141 ccattccctg gaagtacagg ttttcaccac caccaccacc gtgatgatga tgatgatgca
    13201 tcttgtcgac gtaggccttt gaattccgcg cgcttcggac cgggatccgc gcccgatggt
    13261 gggacggtat gaataatccg gaatatttat aggttttttt attacaaaac tgttacgaaa
    13321 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatctat
    13381 taatattccg gagtatacgt agccaaccac tagaactata gctagagtcc tgggcgaaca
    13441 aacgatgctc gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca
    13501 ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc
    13561 acagcacctt gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg
    13621 aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg
    13681 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct
    13741 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
    13801 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggctt
//
