LOCUS       sequence-337599-        9504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid to express mouse SNX9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Feng Z, Yu CH
  TITLE     PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking
            and invasive cell migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 11;118(19). pii: 2017645118. doi:
            10.1073/pnas.2017645118.
  PUBMED    33947811
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-11"; volume: "118(19). pii: 
            2017645118. doi"; pages: " 10.1073/pnas.2017645118"
COMMENT     Sequence Label: SNX9
FEATURES             Location/Qualifiers
     source          1..9504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(1932..1952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1937..2266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2117..2252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2276..2875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2276..2295)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2772..2792
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2951..3254
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3255..3458
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3409..3429
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3455..3479
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5346..5934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5399..5419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5817..5828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6025..6258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6330..6451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6367..6386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6421..6440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6491..6626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6646..6665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6647..6665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6675..6692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6675..6691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6684..6706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6833..7288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6920..6939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7130..7151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7314..7418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7419..7487,7488..8279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7637..7656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8450..9038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8939..8958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9192..9209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9326..9347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9362..9367,9368..9385,9386..9392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9400..9416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9405..9427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9424..9440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9424..9440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9459..9479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9461..9479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     1981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     2221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac
     2281 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2341 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     2401 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     2461 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     2521 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     2581 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     2641 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     2701 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     2761 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     2821 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     2881 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgataagctt
     2941 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     3001 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     3061 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     3121 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     3181 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     3241 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     3301 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     3361 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3421 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3481 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagaatggcc
     3541 accaaggctc gggtcatgta cgactttgct gctgagcctg gaaacaatga gctgaccgtt
     3601 actgaaggag aaatcatcac agttacaaac ccgaatgttg gtggaggatg gctggaagga
     3661 aagaacaaca aaggagagca ggggcttgtt cctactgact acgtggaaat tttaccaaac
     3721 gatggaaaag atccgttttc ttgtgggaat tcagtagccg accaagcttt cctcgattcc
     3781 ctgacagcta gcacagctca aaccaactca tcttctgcca acagcaataa ccaggttggt
     3841 ggtggcaatg acccctggac agcctggaat gctcccaaac ctgggaactg ggacagctcc
     3901 gatgcctggg gctccaggac agacggaact agcgctcaga ggaacagcag cgccaacaac
     3961 tgggacacgg gctttggcca cccacaggcc taccaaggac cagcaactgg tgatgatgac
     4021 gaatgggatg aagactggga cgaccccaag tcctcctcac cttacttcaa ggattcagaa
     4081 ccagctgaag cagggggcat ccagcgggga aacagtcgtg cgggtgcctc ctccatgaag
     4141 ctgcccctta acaaatttcc tggatttgca aaacctggca tggagcagta cttgttggcc
     4201 aagcagctag caaaacccaa agagaaaatc gccatcatcg ttggagatta tggccctatg
     4261 tgggtttatc ctacctctac gtttgactgt gtggtagctg atccccggaa aggttccaaa
     4321 atgtacggtc tgaagagcta cattgagtat cagctaacac ccactaacac taaccgatct
     4381 gtaaaccaca gatataagca ctttgattgg ttatatgagc gtctcctggt taagtttggg
     4441 tctgccattc cgatcccttc tcttccagac aagcaggtca caggtcgctt tgaagaggaa
     4501 tttatcaaaa tgcggatgga gcggctccag gcctggatga ccaggatgtg ccggcacccg
     4561 gtggtgtcag aaagtgaagt tttccagcag ttcctgaatt tccgagatga gaaggaatgg
     4621 aagactggaa agaggaaggc tgagaaagac gaactggtgg gagtgatgat attttccacc
     4681 atggaaccag aggctcctga cttggatttg atagaaatag aacagaagtg tgacgctgtg
     4741 gggaagttca ccaaggccat ggatgacggc gtgaaggagc tgctgacggt ggggcaggag
     4801 cactggaagc gctgcacagg gcccttaccc aaggagtatc agaagatagg gaaggctctg
     4861 cagagcttag cggcagtgtt cagctccagc ggttatcaag gagagaccga ccttaacgat
     4921 gcaatcacag aagctgggaa gacgtatgag gagatcgcca gccttgtggc agagcagcca
     4981 aagaaggacc tccacttcct gatggagtgc aaccatgagt acaaaggctt tctcggctgc
     5041 ttcccggaca tcattggtgc ccacaaggga gcaatagaaa aagtgaaaga aagtgataag
     5101 ctggttgcaa ctagtaaaat tactccccaa gacaagcaga ccatggtgaa gagagttggc
     5161 accatgtctt acgctctgca agctgagatg aatcactttc acagtaaccg gatctatgat
     5221 tacaacagtg tcatccgcct ctacctggag cagcaagtgc agttctatga aacaatcgca
     5281 gaaaagctga ggcaggccct cagccgcttc ccggttatgt aactcgagca ccagaattcg
     5341 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5401 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5461 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5521 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5581 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5641 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5701 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     5761 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5821 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5881 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     5941 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6001 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6241 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6601 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6661 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7261 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9481 aacaaaagct ggagctgcaa gctt
//
