LOCUS       sequence-337602-        9441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses residues 1-1547 of CHD7  in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7 (1-1547)
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(80..85,86..102,103..108))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        460..551
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     476..493
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5276..5299
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5487..5621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5524..5543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5578..5597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5650..5815
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5999..6453
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6086..6105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6295..6316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6479..6583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6584..6652,6653..7444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6802..6821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7615..8203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8104..8123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8357..8374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  8508..8732
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8799..9332)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
       61 catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc
      121 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc
      181 atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt
      241 ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga
      301 gatcggaaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt
      361 tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag
      421 ttctagtggt tggctacgta tactccggaa tattaataga tcatggagat aattaaaatg
      481 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
      541 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga tcccggtccg
      601 aagcgcgcgg aattcaaagg cctacgtcga caagatggca gatccaggaa tgatgagtct
      661 ttttggcgag gatgggaata ttttcagtga aggtcttgaa ggcctcggag aatgtggtta
      721 cccggaaaat ccagtaaatc ctatgggtca gcaaatgcca atagaccaag gctttgcctc
      781 tttacagcca tcccttcatc atccttcaac taatcaaaat caaacaaagc tgacacattt
      841 tgatcactat aatcagtatg aacaacaaaa gatgcatctg atggatcagc cgaacagaat
      901 gatgagcaac acccctggga acggactcgc gtctccgcac tcgcagtatc acacccctcc
      961 cgttcctcag gtgccccatg gtggcagtgg tggcggtcag atgggtgtct accctggcat
     1021 gcagaatgag aggcatgggc aatcctttgt ggacagcagc tccatgtggg gccccagggc
     1081 tgttcaggta ccagaccaga tacgagcccc ctaccagcag cagcagccac agccgcagcc
     1141 accgcagccg gctccgtcgg ggccccctgc acagggccac cctcagcaca tgcagcagat
     1201 gggcagctat atggcacgtg gggatttttc catgcagcag catggtcagc cacagcagag
     1261 gatgagccag ttttcccaag gccaagaggg cctcaatcag ggaaatcctt ttattgccac
     1321 ctcaggacct ggccacttgt cccacgtgcc ccagcagagt cccagcatgg caccttcctt
     1381 gcgtcactcg gtgcagcagt tccatcacca cccctctact gctctccatg gagaatccgt
     1441 tgcccacagt cccagattct ccccgaatcc tccccaacaa ggggctgtta ggccgcaaac
     1501 ccttaacttt agttctcgga gccagacagt cccctctcct actataaaca actcagggca
     1561 gtattctcga tatccttaca gtaacctaaa tcagggatta gttaacaata cagggatgaa
     1621 tcaaaattta ggccttacaa ataatactcc aatgaatcag tccgtaccaa gataccccaa
     1681 tgctgtagga ttcccatcaa acagtggtca aggactaatg caccagcagc ccatccaccc
     1741 cagtggctca cttaaccaaa tgaacacaca aactatgcat ccttcacagc ctcagggaac
     1801 ttatgcctct ccacctccca tgtcacccat gaaagcaatg agtaatccag caggcactcc
     1861 tcctccacaa gtcaggccgg gaagtgctgg gataccaatg gaagttggca gttatccaaa
     1921 tatgccccat cctcagccat ctcaccagcc ccctggtgcc atgggaatcg gacagaggaa
     1981 tatgggcccc agaaacatgc agcagtctcg tccatttata ggcatgtcct cggcaccaag
     2041 ggaattgact gggcacatga ggccaaatgg ttgtcctggt gttggccttg gagacccaca
     2101 agcaatccag gaacgactga tacctggcca acaacatcct ggtcaacagc catcttttca
     2161 gcagttgcca acctgtcctc cactgcagcc tcacccgggc ttgcaccacc agtcttcacc
     2221 tccacaccct catcaccagc cttgggcaca gctccaccca tcaccccaga acaccccgca
     2281 gaaagtgcct gtgcatcagc attccccgtc ggagcccttt ctagagaaac cagtgccgga
     2341 tatgactcag gttagtggac cgaatgctca gctagtgaag agtgatgatt acctgccatc
     2401 aatagaacag cagccacaac aaaagaagaa gaaaaagaaa aacaaccaca ttgtagcaga
     2461 ggatcccagt aaaggttttg gtaaagatga cttccctggt ggggtagata accaagaact
     2521 aaataggaac tcactggatg ggtcccaaga agaaaaaaag aaaaagaaaa ggtcaaaggc
     2581 aaaaaaagac ccgaaggaac cgaaagaacc caaggagaaa aaagagccca aggaacccaa
     2641 gaccccgaaa gcccctaaga ttcccaaaga gccaaaggaa aagaaagcaa aaactgccac
     2701 gccaaaaccc aaatccagca aaaagtcaag taataagaaa cctgactcag aagcaagtgc
     2761 tttgaagaaa aaggtcaaca agggaaaaac agaaggttct gaaaattcag acttagacaa
     2821 aacaccccca ccatctcctc ctcctgaaga agatgaggac ccaggtgttc agaagagacg
     2881 gtccagcaga caggtgaaga gaaagcgcta cactgaagac ctggagttca agatttctga
     2941 tgaggaggca gatgatgcag atgctgctgg gagggattcc ccctccaaca cctcccagtc
     3001 agaacagcag gaatctgttg atgcagaagg cccagtggta gaaaaaatta tgagcagtcg
     3061 ttcagtaaaa aagcagaagg aatctggaga ggaggtagaa attgaggaat tctatgtgaa
     3121 atacaaaaac ttctcttatc ttcattgtca gtgggcatct atagaagatc tggaaaaaga
     3181 taagagaatt cagcaaaaaa ttaaacgatt taaggcaaag cagggccaga acaagttcct
     3241 ttcagagatt gaggatgagc tttttaatcc agattatgtg gaggttgacc ggataatgga
     3301 ctttgcacgt agcacagatg accggggaga gcctgtgact cactatctgg tgaagtggtg
     3361 ttcacttcct tatgaagaca gcacgtggga gcggaggcag gacatagatc aagcaaagat
     3421 cgaggagttt gagaaactaa tgtccaggga gccggaaaca gagcgtgtgg agcgacctcc
     3481 tgctgatgat tggaagaaat cggagagttc cagggagtat aaaaacaata acaaactcag
     3541 ggaataccag ttggagggag taaactggct acttttcaat tggtacaaca tgcgaaactg
     3601 cattttagca gatgaaatgg gtttgggaaa aactatccag tccattacat ttctctatga
     3661 gatatatttg aaaggaatcc atggcccttt tttagtaatt gccccattgt ccacaatccc
     3721 caactgggaa agggaattcc gaacctggac agagttgaac gtggttgtgt atcatgggag
     3781 tcaagctagt cgtcggacca ttcagttgta tgaaatgtac ttcaaagatc cccagggtcg
     3841 agtgataaag gggtcctata agtttcatgc catcatcact acatttgaga tgattttgac
     3901 tgattgtcct gagctgcgga atattccatg gcgctgtgta gtcattgatg aagcccacag
     3961 gctgaagaac aggaactgca agctgttgga gggactcaag atgatggact tggaacacaa
     4021 agtgctgctg acgggaaccc cactccagaa cactgtggaa gaactcttca gcttgcttca
     4081 tttcttggaa ccaagtcgct tcccttcaga aaccacattt atgcaagaat ttggtgatct
     4141 aaaaacagaa gagcaggtgc aaaaacttca agctattcta aagccaatga tgttgagacg
     4201 tctcaaagag gatgtagaaa agaacttggc ccccaaagaa gaaactatta ttgaagttga
     4261 gctaacaaac attcagaaga aatattaccg agccatcctt gagaagaatt tcacatttct
     4321 ttccaaaggc ggtggtcaag ctaacgtacc taacctatta aacactatga tggaattgcg
     4381 gaagtgctgc aatcatccgt accttatcaa tggtgctgaa gagaaaattt tggaagagtt
     4441 taaagaaaca cacaatgcag agtctccaga ttttcagctc caggcaatga tccaggctgc
     4501 tggcaagcta gtgctgattg acaagctgct gccaaaactg aaggctggtg gccacagggt
     4561 gcttatcttt tcccagatgg tgcgctgctt ggacatactg gaagactacc tcattcaaag
     4621 acggtaccca tatgaaagga tcgacggccg agtaagaggc aacctccgcc aggcagctat
     4681 cgacagattc tccaaacctg attctgatag gtttgttttc ctcctgtgta caagggcagg
     4741 aggtttaggc attaacctca ctgctgctga tacctgcatc atctttgatt cagactggaa
     4801 tccccaaaat gacctccagg ctcaggctag atgtcataga ataggacaga gcaaatctgt
     4861 gaaaatctac aggctgatta caagaaattc ctatgaaagg gaaatgttcg acaaggctag
     4921 tttgaaactg ggcctggata aagctgtgct acagtctatg agtggaagag aaaatgctac
     4981 caatggggta caacagcttt ccaagaaaga aatagaggat cttctacgaa aaggggccta
     5041 tggtgcactc atggatgagg aggatgaagg gtctaaattc tgtgaagaag atattgatca
     5101 gatcctccta cgtcgaaccc acaccattac cattgagtca gaagggaaag gttccacatt
     5161 tgctaaggcc agttttgttg catctggaaa taggacagat atttccttgg atgatccaaa
     5221 tttctggcaa aagtgggcta agaaggctga attggatatt gatgccttaa atggggacta
     5281 caaggacgac gatgacaagt agactagtcg cggccgcttt cgaatctaga gcctgcagtc
     5341 tcgaggcatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca
     5401 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     5461 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5581 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
     5641 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     5701 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     5761 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     5821 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     5881 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
     5941 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac
     6001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac
     6301 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6421 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8341 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8401 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     8461 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     8521 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     8581 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     8641 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     8701 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     8761 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     8821 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     8881 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     8941 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     9001 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     9061 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     9121 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     9181 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     9241 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     9301 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     9361 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     9421 gtcataacaa gccatgaaaa c
//
