LOCUS       sequence-337605-       13848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CHD7 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7
FEATURES             Location/Qualifiers
     source          1..13848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(512..517,518..534,535..540))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        892..983
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     908..925
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1070..1087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1103..1123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      1465..1474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10115..10138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    10326..10460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10363..10382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10417..10436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  10489..10654
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      10838..11292
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10925..10944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11134..11155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11318..11422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11423..11491,11492..12283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11641..11660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12454..13042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12943..12962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13196..13213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  13347..13571
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(13638..13848,1..323))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
       61 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
      121 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
      181 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
      241 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
      301 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
      421 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
      481 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
      541 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
      601 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
      661 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
      721 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
      781 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
      841 ctctagctat agttctagtg gttggctacg tatactccgg aatattaata gatcatggag
      901 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
      961 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
     1021 gatcccggtc cgaagcgcgc ggaattcaaa ggcctacgtc gacaagatgc atcatcatca
     1081 tcatcacggt ggtggtggtg gtgaaaacct gtacttccag ggaatggcag atccaggaat
     1141 gatgagtctt tttggcgagg atgggaatat tttcagtgaa ggtcttgaag gcctcggaga
     1201 atgtggttac ccggaaaatc cagtaaatcc tatgggtcag caaatgccaa tagaccaagg
     1261 ctttgcctct ttacagccat cccttcatca tccttcaact aatcaaaatc aaacaaagct
     1321 gacacatttt gatcactata atcagtatga acaacaaaag atgcatctga tggatcagcc
     1381 gaacagaatg atgagcaaca cccctgggaa cggactcgcg tctccgcact cgcagtatca
     1441 cacccctccc gttcctcagg tgccccatgg tggcagtggt ggcggtcaga tgggtgtcta
     1501 ccctggcatg cagaatgaga ggcatgggca atcctttgtg gacagcagct ccatgtgggg
     1561 ccccagggct gttcaggtac cagaccagat acgagccccc taccagcagc agcagccaca
     1621 gccgcagcca ccgcagccgg ctccgtcggg gccccctgca cagggccacc ctcagcacat
     1681 gcagcagatg ggcagctata tggcacgtgg ggatttttcc atgcagcagc atggtcagcc
     1741 acagcagagg atgagccagt tttcccaagg ccaagagggc ctcaatcagg gaaatccttt
     1801 tattgccacc tcaggacctg gccacttgtc ccacgtgccc cagcagagtc ccagcatggc
     1861 accttccttg cgtcactcgg tgcagcagtt ccatcaccac ccctctactg ctctccatgg
     1921 agaatccgtt gcccacagtc ccagattctc cccgaatcct ccccaacaag gggctgttag
     1981 gccgcaaacc cttaacttta gttctcggag ccagacagtc ccctctccta ctataaacaa
     2041 ctcagggcag tattctcgat atccttacag taacctaaat cagggattag ttaacaatac
     2101 agggatgaat caaaatttag gccttacaaa taatactcca atgaatcagt ccgtaccaag
     2161 ataccccaat gctgtaggat tcccatcaaa cagtggtcaa ggactaatgc accagcagcc
     2221 catccacccc agtggctcac ttaaccaaat gaacacacaa actatgcatc cttcacagcc
     2281 tcagggaact tatgcctctc cacctcccat gtcacccatg aaagcaatga gtaatccagc
     2341 aggcactcct cctccacaag tcaggccggg aagtgctggg ataccaatgg aagttggcag
     2401 ttatccaaat atgccccatc ctcagccatc tcaccagccc cctggtgcca tgggaatcgg
     2461 acagaggaat atgggcccca gaaacatgca gcagtctcgt ccatttatag gcatgtcctc
     2521 ggcaccaagg gaattgactg ggcacatgag gccaaatggt tgtcctggtg ttggccttgg
     2581 agacccacaa gcaatccagg aacgactgat acctggccaa caacatcctg gtcaacagcc
     2641 atcttttcag cagttgccaa cctgtcctcc actgcagcct cacccgggct tgcaccacca
     2701 gtcttcacct ccacaccctc atcaccagcc ttgggcacag ctccacccat caccccagaa
     2761 caccccgcag aaagtgcctg tgcatcagca ttccccgtcg gagccctttc tagagaaacc
     2821 agtgccggat atgactcagg ttagtggacc gaatgctcag ctagtgaaga gtgatgatta
     2881 cctgccatca atagaacagc agccacaaca aaagaagaag aaaaagaaaa acaaccacat
     2941 tgtagcagag gatcccagta aaggttttgg taaagatgac ttccctggtg gggtagataa
     3001 ccaagaacta aataggaact cactggatgg gtcccaagaa gaaaaaaaga aaaagaaaag
     3061 gtcaaaggca aaaaaagacc cgaaggaacc gaaagaaccc aaggagaaaa aagagcccaa
     3121 ggaacccaag accccgaaag cccctaagat tcccaaagag ccaaaggaaa agaaagcaaa
     3181 aactgccacg ccaaaaccca aatccagcaa aaagtcaagt aataagaaac ctgactcaga
     3241 agcaagtgct ttgaagaaaa aggtcaacaa gggaaaaaca gaaggttctg aaaattcaga
     3301 cttagacaaa acacccccac catctcctcc tcctgaagaa gatgaggacc caggtgttca
     3361 gaagagacgg tccagcagac aggtgaagag aaagcgctac actgaagacc tggagttcaa
     3421 gatttctgat gaggaggcag atgatgcaga tgctgctggg agggattccc cctccaacac
     3481 ctcccagtca gaacagcagg aatctgttga tgcagaaggc ccagtggtag aaaaaattat
     3541 gagcagtcgt tcagtaaaaa agcagaagga atctggagag gaggtagaaa ttgaggaatt
     3601 ctatgtgaaa tacaaaaact tctcttatct tcattgtcag tgggcatcta tagaagatct
     3661 ggaaaaagat aagagaattc agcaaaaaat taaacgattt aaggcaaagc agggccagaa
     3721 caagttcctt tcagagattg aggatgagct ttttaatcca gattatgtgg aggttgaccg
     3781 gataatggac tttgcacgta gcacagatga ccggggagag cctgtgactc actatctggt
     3841 gaagtggtgt tcacttcctt atgaagacag cacgtgggag cggaggcagg acatagatca
     3901 agcaaagatc gaggagtttg agaaactaat gtccagggag ccggaaacag agcgtgtgga
     3961 gcgacctcct gctgatgatt ggaagaaatc ggagagttcc agggagtata aaaacaataa
     4021 caaactcagg gaataccagt tggagggagt aaactggcta cttttcaatt ggtacaacat
     4081 gcgaaactgc attttagcag atgaaatggg tttgggaaaa actatccagt ccattacatt
     4141 tctctatgag atatatttga aaggaatcca tggccctttt ttagtaattg ccccattgtc
     4201 cacaatcccc aactgggaaa gggaattccg aacctggaca gagttgaacg tggttgtgta
     4261 tcatgggagt caagctagtc gtcggaccat tcagttgtat gaaatgtact tcaaagatcc
     4321 ccagggtcga gtgataaagg ggtcctataa gtttcatgcc atcatcacta catttgagat
     4381 gattttgact gattgtcctg agctgcggaa tattccatgg cgctgtgtag tcattgatga
     4441 agcccacagg ctgaagaaca ggaactgcaa gctgttggag ggactcaaga tgatggactt
     4501 ggaacacaaa gtgctgctga cgggaacccc actccagaac actgtggaag aactcttcag
     4561 cttgcttcat ttcttggaac caagtcgctt cccttcagaa accacattta tgcaagaatt
     4621 tggtgatcta aaaacagaag agcaggtgca aaaacttcaa gctattctaa agccaatgat
     4681 gttgagacgt ctcaaagagg atgtagaaaa gaacttggcc cccaaagaag aaactattat
     4741 tgaagttgag ctaacaaaca ttcagaagaa atattaccga gccatccttg agaagaattt
     4801 cacatttctt tccaaaggcg gtggtcaagc taacgtacct aacctattaa acactatgat
     4861 ggaattgcgg aagtgctgca atcatccgta ccttatcaat ggtgctgaag agaaaatttt
     4921 ggaagagttt aaagaaacac acaatgcaga gtctccagat tttcagctcc aggcaatgat
     4981 ccaggctgct ggcaagctag tgctgattga caagctgctg ccaaaactga aggctggtgg
     5041 ccacagggtg cttatctttt cccagatggt gcgctgcttg gacatactgg aagactacct
     5101 cattcaaaga cggtacccat atgaaaggat cgacggccga gtaagaggca acctccgcca
     5161 ggcagctatc gacagattct ccaaacctga ttctgatagg tttgttttcc tcctgtgtac
     5221 aagggcagga ggtttaggca ttaacctcac tgctgctgat acctgcatca tctttgattc
     5281 agactggaat ccccaaaatg acctccaggc tcaggctaga tgtcatagaa taggacagag
     5341 caaatctgtg aaaatctaca ggctgattac aagaaattcc tatgaaaggg aaatgttcga
     5401 caaggctagt ttgaaactgg gcctggataa agctgtgcta cagtctatga gtggaagaga
     5461 aaatgctacc aatggggtac aacagctttc caagaaagaa atagaggatc ttctacgaaa
     5521 aggggcctat ggtgcactca tggatgagga ggatgaaggg tctaaattct gtgaagaaga
     5581 tattgatcag atcctcctac gtcgaaccca caccattacc attgagtcag aagggaaagg
     5641 ttccacattt gctaaggcca gttttgttgc atctggaaat aggacagata tttccttgga
     5701 tgatccaaat ttctggcaaa agtgggctaa gaaggctgaa ttggatattg atgccttaaa
     5761 tgggaggaac aacctggtta ttgatactcc aagagtgaga aagcagacca ggctctacag
     5821 tgcagtgaag gaagatgagc tgatggagtt ctcagacttg gaaagtgatt ctgaagaaaa
     5881 gccctgtgca aagccacggc gtccccagga taagtcacag ggctatgcaa ggagtgaatg
     5941 tttcagggtg gagaagaatc tgcttgtcta tggttgggga cggtggacag acattctttc
     6001 ccacggacgc tataaacgcc aactcactga gcaagatgta gaaaccatct gcagaaccat
     6061 cctggtgtac tgtcttaatc attacaaagg ggatgagaat atcaaaagct tcatctggga
     6121 tctgatcaca cccacagcgg atggccagac tcgagccttg gtcaaccatt ccggtttgtc
     6181 agctcctgtg ccaaggggaa ggaagggaaa gaaggtgaaa gcccagagca cacagccggt
     6241 ggtgcaggat gccgactggc tggccagctg caacccagat gccctgttcc aggaggacag
     6301 ctacaagaaa cacctgaagc atcactgtaa caaggtcctg ctgcgtgtcc gcatgctgta
     6361 ctacctaaga caagaagtga taggagacca ggcggataag atcttagagg gtgctgactc
     6421 aagtgaagcc gatgtgtgga tccctgaacc tttccatgct gaagttcctg cagattggtg
     6481 ggataaggaa gcagacaaat ccctcttaat tggagtgttc aaacatggct atgagaagta
     6541 caactccatg cgagctgacc ccgcgctgtg ctttctggaa cgagtcggta tgcctgatgc
     6601 caaggccata gctgccgagc aaagaggaac agacatgcta gcagatggtg gtgacggggg
     6661 agaatttgat agagaagatg aagacccaga atataaacca accagaacac cgttcaaaga
     6721 tgaaatagat gaatttgcaa attctccttc agaggataag gaagaatcca tggaaataca
     6781 tgccacaggc aagcacagtg agagtaatgc tgagttaggc caactttact ggcctaacac
     6841 ttcaaccctg actacacgtc tgcgccggct cattactgcc tatcagcgca gctataaaag
     6901 gcaacagatg aggcaagagg ccctaatgaa gactgaccgg cgcagacggc ggcctcgaga
     6961 ggaagtgaga gctctggaag cggaaaggga agctattata tctgagaagc ggcaaaagtg
     7021 gacaagaaga gaagaggctg atttttaccg tgtggtatcc acctttgggg ttatttttga
     7081 ccctgtgaaa cagcaatttg actggaacca atttagagcc tttgccaggc ttgacaaaaa
     7141 atctgatgag agtttggaga aatacttcag ttgttttgtg gccatgtgta ggcgagtatg
     7201 tcgaatgccc gtcaagccag atgatgaacc gcccgacctc tcctccataa ttgagccgat
     7261 cacagaggag cgagcctctc gaactctgta ccgcattgag ctgctacgga agatccgcga
     7321 gcaggttctc catcaccccc agctgggaga gaggcttaag ctctgccagc caagcttgga
     7381 tctgccagag tggtgggagt gtggacggca tgaccgagac ttgctggttg gtgctgctaa
     7441 acacggggtc agtcggacgg attatcacat cctcaatgac cctgagttat ccttcttgga
     7501 tgcacataaa aactttgctc aaaacagagg ggcaggtaat acatcttcct tgaacccact
     7561 ggcagttgga tttgtccaga ctcctccagt catctcatct gctcatattc aagatgagag
     7621 ggtactggaa caagccgaag gcaaagtgga ggagcctgaa aacccagctg ccaaggagaa
     7681 atgtgagggc aaagaagagg aagaagaaac cgatggcagc gggaaggaga gcaagcagga
     7741 atgtgaggca gaggccagct ctgtgaaaaa tgaactgaaa ggtgttgagg tcggcgcaga
     7801 cactgggtcc aaatctattt cagagaaagg ttccgaagag gatgaagagg aaaagctgga
     7861 ggatgacgat aagtcggaag agtcttccca gcccgaagca ggagctgtct ctagagggaa
     7921 gaattttgat gaagaaagca atgcttccat gagcactgct agagatgaaa cccgagatgg
     7981 attctacatg gaggacggag atccttcagt agctcagctc cttcatgaaa gaacatttgc
     8041 cttctcgttt tggcctaagg atagagtaat gataaaccgc ttagacaaca tctgtgaagc
     8101 agtgttgaaa ggcaaatggc cagtaaatag gcgccagatg tttgatttcc aaggcctcat
     8161 cccaggttac acacccacca cagtggacag ccccttgcag aagaggagct ttgctgagct
     8221 ctccatggtc ggccaagcca gcattagtgg gagtgaggac atcactacgt ctcctcagtt
     8281 gtcaaaggaa gatgccctca acctctctgt ccctcgccag cggaggagga ggaggagaaa
     8341 aatcgaaatt gaggccgaaa gagctgccaa gaggcgaaat ctcatggaga tggttgccca
     8401 gcttcgagag tctcaggtgg tctcagaaaa tggacaagaa aaagttgtag atttatcaaa
     8461 ggcctcaaga gaggcaacaa gctctacctc aaatttttca tctctttctt caaagtttat
     8521 cttgcctaat gtctcaacac cagtgtctga tgcctttaag actcaaatgg aactgctcca
     8581 agcaggcctt tcgcgcacac ccacaaggca tctccttaat ggctccctag tggatggaga
     8641 gcctcccatg aagaggaggc ggggaaggag gaaaaatgtg gagggacttg atctgctttt
     8701 catgagccac aaacggacgt cattgagtgc agaggatgct gaggtgacca aagcttttga
     8761 agaagatata gagaccccac caacaagaaa cattccttct cccggacagc tggacccaga
     8821 cacacggatc cctgttatca atcttgaaga tgggactagg ctggtggggg aagatgctcc
     8881 taaaaataag gatttagttg aatggctgaa gctgcaccct acttacactg ttgatatgcc
     8941 aagttatgta ccaaagaatg cagatgtgct gttttcctca tttcagaaac cgaaacagaa
     9001 acgacataga tgtcgaaacc ctaataaatt ggatataaac actttgacag gagaagaaag
     9061 ggtgcctgtt gtcaataaac gaaatgggaa gaagatgggt ggagctatgg cgcctccaat
     9121 gaaggatcta cccaggtggc tggaagaaaa tcctgaattt gcagttgctc cagactggac
     9181 tgatatagtt aagcagtctg gttttgttcc tgagtcgatg tttgaccgcc ttctcactgg
     9241 gcctgtagtg cggggagagg gagcgagcag aagaggaaga aggcccaaaa gtgagatcgc
     9301 cagagcagcc gcggccgccg ctgctgtggc ctccacgtca gggatcaacc ctttgctggt
     9361 gaacagcctg tttgctggaa tggacctgac gagccttcag aatctccaga atctccagtc
     9421 gctccagctg gcaggcctca tgggcttccc tccaggactg gcaacagctg ccaccgccgg
     9481 aggcgatgcg aagaaccctg ctgctgtgct gcccctgatg ctgccaggaa tggcgggcct
     9541 gcccaacgtg tttggcttgg gcgggctgtt gaataaccct ctgtcagctg ctactggaaa
     9601 caccactact gcttctagtc aaggagaacc ggaagacagc acttcaaaag gagaggagaa
     9661 aggaaatgag aatgaagacg agaacaaaga ctctgagaaa agcacagatg ctgtttcggc
     9721 tgctgactct gcgaatggat ctgttggtgc tgctactgcc ccggctggat tgccctcaaa
     9781 cccgctagcc ttcaaccctt tcctcctgtc cacaatggcc ccgggcctct tctacccatc
     9841 catgtttcta cctccaggac tggggggatt gacgctgcct gggttcccag cattggcagg
     9901 acttcagaat gccgtgggct ccagcgaaga aaaggctgct gacaaggctg agggaggacc
     9961 ctttaaagat ggagagaccc ttgaaggcag cgatgccgag gagagcctgg ataagactgc
    10021 agagtcctcc ctcttagaag acgaaatagc acagggtgaa gagctagact cacttgatgg
    10081 gggggatgaa atagaaaaca atgaaaatga tgaagactac aaggacgacg atgacaagta
    10141 gactagtcgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
    10201 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
    10261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
    10321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10441 tatcttatca tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt
    10501 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
    10561 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
    10621 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
    10681 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
    10741 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
    10801 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    10861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    11101 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
    11161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    11281 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    11341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    11401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    11461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    11521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    11581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    11641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    11701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    11761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    11821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    11881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    11941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    12001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    12061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    12121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    12181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    12241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    12301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    12481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    12541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    12601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    12661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    12721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    12781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    12841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    12901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    12961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    13021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    13081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    13141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    13201 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
    13261 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
    13321 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
    13381 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
    13441 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
    13501 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
    13561 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
    13621 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
    13681 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
    13741 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg
    13801 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatag
//
