LOCUS       sequence-337606-        8878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses residues 787-2138 of CHD7  in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7 (787-2138)
FEATURES             Location/Qualifiers
     source          1..8878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     373..392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     626..643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  777..1001
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1068..1601)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1809..1814,1815..1831,1832..1837))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2189..2280
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2205..2222
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6423..6446
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6634..6768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6671..6690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6725..6744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6797..6962
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7146..7600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7233..7252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7442..7463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7626..7730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7731..7799,7800..8591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7949..7968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8762..8878,1..472)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
       61 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
      661 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg
      721 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg
      781 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
      841 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
      901 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
      961 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta
     1021 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     1081 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     1141 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     1201 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     1261 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     1321 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     1381 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     1441 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     1501 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     1561 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tgcgtaacat
     1621 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     1681 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     1741 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     1801 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
     1861 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
     1921 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc
     1981 tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac
     2041 ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg
     2101 gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt
     2161 ggctacgtat actccggaat attaatagat catggagata attaaaatga taaccatctc
     2221 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     2281 attccggatt attcataccg tcccaccatc gggcgcggat cccggtccga agcgcgcgga
     2341 attcaaaggc ctacgtcgac aagatgtccc agtcagaaca gcaggaatct gttgatgcag
     2401 aaggcccagt ggtagaaaaa attatgagca gtcgttcagt aaaaaagcag aaggaatctg
     2461 gagaggaggt agaaattgag gaattctatg tgaaatacaa aaacttctct tatcttcatt
     2521 gtcagtgggc atctatagaa gatctggaaa aagataagag aattcagcaa aaaattaaac
     2581 gatttaaggc aaagcagggc cagaacaagt tcctttcaga gattgaggat gagcttttta
     2641 atccagatta tgtggaggtt gaccggataa tggactttgc acgtagcaca gatgaccggg
     2701 gagagcctgt gactcactat ctggtgaagt ggtgttcact tccttatgaa gacagcacgt
     2761 gggagcggag gcaggacata gatcaagcaa agatcgagga gtttgagaaa ctaatgtcca
     2821 gggagccgga aacagagcgt gtggagcgac ctcctgctga tgattggaag aaatcggaga
     2881 gttccaggga gtataaaaac aataacaaac tcagggaata ccagttggag ggagtaaact
     2941 ggctactttt caattggtac aacatgcgaa actgcatttt agcagatgaa atgggtttgg
     3001 gaaaaactat ccagtccatt acatttctct atgagatata tttgaaagga atccatggcc
     3061 cttttttagt aattgcccca ttgtccacaa tccccaactg ggaaagggaa ttccgaacct
     3121 ggacagagtt gaacgtggtt gtgtatcatg ggagtcaagc tagtcgtcgg accattcagt
     3181 tgtatgaaat gtacttcaaa gatccccagg gtcgagtgat aaaggggtcc tataagtttc
     3241 atgccatcat cactacattt gagatgattt tgactgattg tcctgagctg cggaatattc
     3301 catggcgctg tgtagtcatt gatgaagccc acaggctgaa gaacaggaac tgcaagctgt
     3361 tggagggact caagatgatg gacttggaac acaaagtgct gctgacggga accccactcc
     3421 agaacactgt ggaagaactc ttcagcttgc ttcatttctt ggaaccaagt cgcttccctt
     3481 cagaaaccac atttatgcaa gaatttggtg atctaaaaac agaagagcag gtgcaaaaac
     3541 ttcaagctat tctaaagcca atgatgttga gacgtctcaa agaggatgta gaaaagaact
     3601 tggcccccaa agaagaaact attattgaag ttgagctaac aaacattcag aagaaatatt
     3661 accgagccat ccttgagaag aatttcacat ttctttccaa aggcggtggt caagctaacg
     3721 tacctaacct attaaacact atgatggaat tgcggaagtg ctgcaatcat ccgtacctta
     3781 tcaatggtgc tgaagagaaa attttggaag agtttaaaga aacacacaat gcagagtctc
     3841 cagattttca gctccaggca atgatccagg ctgctggcaa gctagtgctg attgacaagc
     3901 tgctgccaaa actgaaggct ggtggccaca gggtgcttat cttttcccag atggtgcgct
     3961 gcttggacat actggaagac tacctcattc aaagacggta cccatatgaa aggatcgacg
     4021 gccgagtaag aggcaacctc cgccaggcag ctatcgacag attctccaaa cctgattctg
     4081 ataggtttgt tttcctcctg tgtacaaggg caggaggttt aggcattaac ctcactgctg
     4141 ctgatacctg catcatcttt gattcagact ggaatcccca aaatgacctc caggctcagg
     4201 ctagatgtca tagaatagga cagagcaaat ctgtgaaaat ctacaggctg attacaagaa
     4261 attcctatga aagggaaatg ttcgacaagg ctagtttgaa actgggcctg gataaagctg
     4321 tgctacagtc tatgagtgga agagaaaatg ctaccaatgg ggtacaacag ctttccaaga
     4381 aagaaataga ggatcttcta cgaaaagggg cctatggtgc actcatggat gaggaggatg
     4441 aagggtctaa attctgtgaa gaagatattg atcagatcct cctacgtcga acccacacca
     4501 ttaccattga gtcagaaggg aaaggttcca catttgctaa ggccagtttt gttgcatctg
     4561 gaaataggac agatatttcc ttggatgatc caaatttctg gcaaaagtgg gctaagaagg
     4621 ctgaattgga tattgatgcc ttaaatggga ggaacaacct ggttattgat actccaagag
     4681 tgagaaagca gaccaggctc tacagtgcag tgaaggaaga tgagctgatg gagttctcag
     4741 acttggaaag tgattctgaa gaaaagccct gtgcaaagcc acggcgtccc caggataagt
     4801 cacagggcta tgcaaggagt gaatgtttca gggtggagaa gaatctgctt gtctatggtt
     4861 ggggacggtg gacagacatt ctttcccacg gacgctataa acgccaactc actgagcaag
     4921 atgtagaaac catctgcaga accatcctgg tgtactgtct taatcattac aaaggggatg
     4981 agaatatcaa aagcttcatc tgggatctga tcacacccac agcggatggc cagactcgag
     5041 ccttggtcaa ccattccggt ttgtcagctc ctgtgccaag gggaaggaag ggaaagaagg
     5101 tgaaagccca gagcacacag ccggtggtgc aggatgccga ctggctggcc agctgcaacc
     5161 cagatgccct gttccaggag gacagctaca agaaacacct gaagcatcac tgtaacaagg
     5221 tcctgctgcg tgtccgcatg ctgtactacc taagacaaga agtgatagga gaccaggcgg
     5281 ataagatctt agagggtgct gactcaagtg aagccgatgt gtggatccct gaacctttcc
     5341 atgctgaagt tcctgcagat tggtgggata aggaagcaga caaatccctc ttaattggag
     5401 tgttcaaaca tggctatgag aagtacaact ccatgcgagc tgaccccgcg ctgtgctttc
     5461 tggaacgagt cggtatgcct gatgccaagg ccatagctgc cgagcaaaga ggaacagaca
     5521 tgctagcaga tggtggtgac gggggagaat ttgatagaga agatgaagac ccagaatata
     5581 aaccaaccag aacaccgttc aaagatgaaa tagatgaatt tgcaaattct ccttcagagg
     5641 ataaggaaga atccatggaa atacatgcca caggcaagca cagtgagagt aatgctgagt
     5701 taggccaact ttactggcct aacacttcaa ccctgactac acgtctgcgc cggctcatta
     5761 ctgcctatca gcgcagctat aaaaggcaac agatgaggca agaggcccta atgaagactg
     5821 accggcgcag acggcggcct cgagaggaag tgagagctct ggaagcggaa agggaagcta
     5881 ttatatctga gaagcggcaa aagtggacaa gaagagaaga ggctgatttt taccgtgtgg
     5941 tatccacctt tggggttatt tttgaccctg tgaaacagca atttgactgg aaccaattta
     6001 gagcctttgc caggcttgac aaaaaatctg atgagagttt ggagaaatac ttcagttgtt
     6061 ttgtggccat gtgtaggcga gtatgtcgaa tgcccgtcaa gccagatgat gaaccgcccg
     6121 acctctcctc cataattgag ccgatcacag aggagcgagc ctctcgaact ctgtaccgca
     6181 ttgagctgct acggaagatc cgcgagcagg ttctccatca cccccagctg ggagagaggc
     6241 ttaagctctg ccagccaagc ttggatctgc cagagtggtg ggagtgtgga cggcatgacc
     6301 gagacttgct ggttggtgct gctaaacacg gggtcagtcg gacggattat cacatcctca
     6361 atgaccctga gttatccttc ttggatgcac ataaaaactt tgctcaaaac agaggggcag
     6421 gtgactacaa ggacgacgat gacaagtaga ctagtcgcgg ccgctttcga atctagagcc
     6481 tgcagtctcg aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     6541 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6601 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6721 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     6781 gagcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     6841 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     6901 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     6961 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     7021 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     7081 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
     7141 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7201 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7261 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7321 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7381 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
     7441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactgg
//
