LOCUS       sequence-337608-        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses residues 787-1899 of CHD7  in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7 (787-1899)
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(415..420,421..437,438..443))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        795..886
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     811..828
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4312..4335
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4523..4657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4560..4579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4614..4633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4686..4851
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5035..5489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5122..5141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5331..5352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5515..5619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5620..5688,5689..6480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5838..5857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7140..7159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7393..7410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7544..7768
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(7835..8142,1..226))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
       61 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
      121 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
      181 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctgcgt
      241 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
      301 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
      361 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
      421 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
      481 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
      541 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
      601 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
      661 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
      721 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
      781 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
      841 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
      901 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac
      961 gtcgacaaga tgtcccagtc agaacagcag gaatctgttg atgcagaagg cccagtggta
     1021 gaaaaaatta tgagcagtcg ttcagtaaaa aagcagaagg aatctggaga ggaggtagaa
     1081 attgaggaat tctatgtgaa atacaaaaac ttctcttatc ttcattgtca gtgggcatct
     1141 atagaagatc tggaaaaaga taagagaatt cagcaaaaaa ttaaacgatt taaggcaaag
     1201 cagggccaga acaagttcct ttcagagatt gaggatgagc tttttaatcc agattatgtg
     1261 gaggttgacc ggataatgga ctttgcacgt agcacagatg accggggaga gcctgtgact
     1321 cactatctgg tgaagtggtg ttcacttcct tatgaagaca gcacgtggga gcggaggcag
     1381 gacatagatc aagcaaagat cgaggagttt gagaaactaa tgtccaggga gccggaaaca
     1441 gagcgtgtgg agcgacctcc tgctgatgat tggaagaaat cggagagttc cagggagtat
     1501 aaaaacaata acaaactcag ggaataccag ttggagggag taaactggct acttttcaat
     1561 tggtacaaca tgcgaaactg cattttagca gatgaaatgg gtttgggaaa aactatccag
     1621 tccattacat ttctctatga gatatatttg aaaggaatcc atggcccttt tttagtaatt
     1681 gccccattgt ccacaatccc caactgggaa agggaattcc gaacctggac agagttgaac
     1741 gtggttgtgt atcatgggag tcaagctagt cgtcggacca ttcagttgta tgaaatgtac
     1801 ttcaaagatc cccagggtcg agtgataaag gggtcctata agtttcatgc catcatcact
     1861 acatttgaga tgattttgac tgattgtcct gagctgcgga atattccatg gcgctgtgta
     1921 gtcattgatg aagcccacag gctgaagaac aggaactgca agctgttgga gggactcaag
     1981 atgatggact tggaacacaa agtgctgctg acgggaaccc cactccagaa cactgtggaa
     2041 gaactcttca gcttgcttca tttcttggaa ccaagtcgct tcccttcaga aaccacattt
     2101 atgcaagaat ttggtgatct aaaaacagaa gagcaggtgc aaaaacttca agctattcta
     2161 aagccaatga tgttgagacg tctcaaagag gatgtagaaa agaacttggc ccccaaagaa
     2221 gaaactatta ttgaagttga gctaacaaac attcagaaga aatattaccg agccatcctt
     2281 gagaagaatt tcacatttct ttccaaaggc ggtggtcaag ctaacgtacc taacctatta
     2341 aacactatga tggaattgcg gaagtgctgc aatcatccgt accttatcaa tggtgctgaa
     2401 gagaaaattt tggaagagtt taaagaaaca cacaatgcag agtctccaga ttttcagctc
     2461 caggcaatga tccaggctgc tggcaagcta gtgctgattg acaagctgct gccaaaactg
     2521 aaggctggtg gccacagggt gcttatcttt tcccagatgg tgcgctgctt ggacatactg
     2581 gaagactacc tcattcaaag acggtaccca tatgaaagga tcgacggccg agtaagaggc
     2641 aacctccgcc aggcagctat cgacagattc tccaaacctg attctgatag gtttgttttc
     2701 ctcctgtgta caagggcagg aggtttaggc attaacctca ctgctgctga tacctgcatc
     2761 atctttgatt cagactggaa tccccaaaat gacctccagg ctcaggctag atgtcataga
     2821 ataggacaga gcaaatctgt gaaaatctac aggctgatta caagaaattc ctatgaaagg
     2881 gaaatgttcg acaaggctag tttgaaactg ggcctggata aagctgtgct acagtctatg
     2941 agtggaagag aaaatgctac caatggggta caacagcttt ccaagaaaga aatagaggat
     3001 cttctacgaa aaggggccta tggtgcactc atggatgagg aggatgaagg gtctaaattc
     3061 tgtgaagaag atattgatca gatcctccta cgtcgaaccc acaccattac cattgagtca
     3121 gaagggaaag gttccacatt tgctaaggcc agttttgttg catctggaaa taggacagat
     3181 atttccttgg atgatccaaa tttctggcaa aagtgggcta agaaggctga attggatatt
     3241 gatgccttaa atgggaggaa caacctggtt attgatactc caagagtgag aaagcagacc
     3301 aggctctaca gtgcagtgaa ggaagatgag ctgatggagt tctcagactt ggaaagtgat
     3361 tctgaagaaa agccctgtgc aaagccacgg cgtccccagg ataagtcaca gggctatgca
     3421 aggagtgaat gtttcagggt ggagaagaat ctgcttgtct atggttgggg acggtggaca
     3481 gacattcttt cccacggacg ctataaacgc caactcactg agcaagatgt agaaaccatc
     3541 tgcagaacca tcctggtgta ctgtcttaat cattacaaag gggatgagaa tatcaaaagc
     3601 ttcatctggg atctgatcac acccacagcg gatggccaga ctcgagcctt ggtcaaccat
     3661 tccggtttgt cagctcctgt gccaagggga aggaagggaa agaaggtgaa agcccagagc
     3721 acacagccgg tggtgcagga tgccgactgg ctggccagct gcaacccaga tgccctgttc
     3781 caggaggaca gctacaagaa acacctgaag catcactgta acaaggtcct gctgcgtgtc
     3841 cgcatgctgt actacctaag acaagaagtg ataggagacc aggcggataa gatcttagag
     3901 ggtgctgact caagtgaagc cgatgtgtgg atccctgaac ctttccatgc tgaagttcct
     3961 gcagattggt gggataagga agcagacaaa tccctcttaa ttggagtgtt caaacatggc
     4021 tatgagaagt acaactccat gcgagctgac cccgcgctgt gctttctgga acgagtcggt
     4081 atgcctgatg ccaaggccat agctgccgag caaagaggaa cagacatgct agcagatggt
     4141 ggtgacgggg gagaatttga tagagaagat gaagacccag aatataaacc aaccagaaca
     4201 ccgttcaaag atgaaataga tgaatttgca aattctcctt cagaggataa ggaagaatcc
     4261 atggaaatac atgccacagg caagcacagt gagagtaatg ctgagttagg cgactacaag
     4321 gacgacgatg acaagtagac tagtcgcggc cgctttcgaa tctagagcct gcagtctcga
     4381 ggcatgcggt accaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     4441 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     4501 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     4561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4621 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag
     4681 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     4741 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     4801 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     4861 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     4921 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     4981 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
     5041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt
     5341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5461 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     7441 ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa
     7501 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     7561 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     7621 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     7681 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     7741 ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     7801 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     7861 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     7921 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     7981 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     8041 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     8101 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat ga
//
