LOCUS       sequence-337614-       11992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histidine tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_His6_GluA2
FEATURES             Location/Qualifiers
     source          1..11992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2389..2836
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2857..2877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2881..2901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2971..2992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3232..3251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3652..3708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3790..3807
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6447..7035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6500..6520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6918..6929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7038..7054)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7039..7055)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7560..7740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7766..7783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7772..7996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8042..8470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8129..8148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8339..8360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8479..8499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8484..8813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8664..8799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8726..8745
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8861..8908
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8927..9301
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9431..9552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9468..9487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9522..9541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9601..9617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9601..9617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9614..9636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9625..9641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9649..9655,9656..9673,9674..9679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9694..9715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9832..9849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10003..10591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10083..10102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10762..11553,11554..11622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11385..11404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11623..11727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11802..11821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gagagggccc tgcgtatgag tgcaagtggg
     2401 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     2461 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     2521 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     2581 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     2641 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     2701 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     2761 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     2821 gtgcctgaga gcgcagtcgg atccgccacc atggctccaa aaaagaagag aaaggtagat
     2881 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatggt gagcaagggc
     2941 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3001 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3061 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3121 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3181 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3241 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3301 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3361 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3421 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3541 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg agctactaac
     3661 ttcagcctgc tgaagcaagc aggagatgtt gaagaaaacc ccgggcctgc tagcatgcaa
     3721 aagattatgc atatttctgt cctcctttct cctgttttat ggggactgat ttttggtgtc
     3781 tcttctatgc atcatcacca tcaccacaac agcatacaga taggggggct atttccaagg
     3841 ggcgctgatc aagaatacag tgcatttcgg gtagggatgg ttcagttttc cacttcggag
     3901 ttcagactga caccccatat cgacaatttg gaggtagcca acagtttcgc agtcaccaat
     3961 gctttctgct cccagttttc aagaggagtc tacgcaattt ttggatttta tgacaagaag
     4021 tctgtaaata ccatcacatc attctgtggg acactccatg tgtccttcat cacacctagc
     4081 ttcccaacag atggcacaca tccatttgtc atccagatgc gacctgacct caaaggagca
     4141 ctccttagct tgattgagta ctaccaatgg gacaagttcg catacctcta tgacagtgac
     4201 agaggcttat caacactgca agctgttctg gattctgctg cagagaagaa gtggcaggtg
     4261 actgctatca atgtggggaa catcaacaat gacaagaaag atgagaccta cagatcgctc
     4321 tttcaagatc tggagttaaa aaaagaacgg cgtgtaatcc tggactgtga aagggataaa
     4381 gtaaatgaca ttgtggacca ggttattacc attggaaaac atgttaaagg gtaccattat
     4441 atcattgcaa atctgggatt cactgatggg gacctgctga aaattcagtt tggaggagca
     4501 aatgtctctg gatttcagat tgtagactac gatgattccc tggtgtctaa atttatagaa
     4561 agatggtcaa cactggaaga gaaagaatac cctggagcac acacagcgac aattaagtat
     4621 acttcggccc tgacgtatga tgctgtccaa gtgatgactg aagcattccg taaccttcgg
     4681 aagcagagga ttgaaatatc ccggagagga aatgcagggg attgtttggc caacccagct
     4741 gtgccctggg gacaaggggt cgaaatagaa agggccctca agcaggttca agttgaaggc
     4801 ctctctggaa atataaagtt tgaccagaat ggaaaacgaa taaactacac aattaacatc
     4861 atggagctca aaacaaatgg accccggaag attgggtact ggagtgaagt ggataaaatg
     4921 gttgtcaccc taactgagct cccatcagga aatgacacgt ctgggcttga aaacaagact
     4981 gtggtggtca ccacaatatt ggaatctcca tatgttatga tgaagaaaaa tcatgaaatg
     5041 cttgaaggga atgagcgtta cgagggctac tgtgttgact tagctgcaga aattgccaaa
     5101 cactgtgggt tcaagtacaa gctgactatt gttggggatg gcaagtatgg ggccagggat
     5161 gccgacacca aaatttggaa tggtatggtt ggagagcttg tctacgggaa agctgacatt
     5221 gcaattgctc cattaactat cactctcgtg agagaagagg tgattgactt ctccaagccc
     5281 ttcatgagtc ttggaatctc tatcatgatc aagaagcctc agaagtccaa accaggagtg
     5341 ttttcctttc ttgatccttt agcctatgag atctggatgt gcattgtgtt tgcctacatt
     5401 ggggtcagtg tagttttatt cctggtcagc agatttagcc cctacgagtg gcacactgag
     5461 gaatttgaag atggaagaga aacacaaagt agtgaatcaa ctaatgaatt tgggattttt
     5521 aatagtctct ggttttcctt gggtgccttt atgcggcaag gatgcgatat ttcgccaaga
     5581 tccctctctg ggcgcattgt tggaggtgtg tggtggttct ttaccctgat cataatctcc
     5641 tcctacacgg ctaacttagc tgccttcctg actgtagaga ggatggtgtc tcccatcgaa
     5701 agtgctgagg atctgtctaa gcaaacagaa attgcttatg gaacattaga ctctggctcc
     5761 actaaagagt ttttcaggag atctaaaatc gcagtgtttg ataaaatgtg gacttatatg
     5821 aggagtgcag agccctctgt gtttgtgagg actaccgcag aaggagtagc cagagtccgg
     5881 aaatccaaag gaaagtatgc ctacttgctg gagtccacaa tgaacgagta catcgagcag
     5941 aggaagcctt gtgacaccat gaaagtggga ggaaacttgg attccaaagg ctacggcatc
     6001 gccacaccta aaggatcctc attaggaacc ccagtaaatc ttgcagtatt gaaactcagt
     6061 gagcaaggcg tcttagacaa gctgaaaaac aaatggtggt acgataaagg tgaatgtgga
     6121 gccaaggact cgggaagtaa ggaaaagacc agtgccctca gtctgagcaa cgttgctgga
     6181 gtattctaca tccttgtcgg gggccttggt ttggcaatgc tggtggcttt gattgagttc
     6241 tgttacaagt caagggccga ggcgaaacga atgaaggtgg caaagaatcc acagaatatt
     6301 aacccatctt cctcgcagaa ttcccagaat tttgcaactt ataaggaagg ttacaacgta
     6361 tatggcatcg agagtgttaa aatttagggc gcgccttcga attaattaag gccgcgactc
     6421 tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6481 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6541 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6601 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6661 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6721 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6841 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6901 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6961 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7021 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7081 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7141 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7261 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7321 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7381 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7441 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7501 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7741 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8821 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     8881 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8941 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     9001 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     9061 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     9121 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     9181 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     9241 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     9301 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9361 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9421 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9541 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11761 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    11821 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    11881 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    11941 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
