LOCUS       sequence-337615-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses halo tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_Halo_GluA2
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2389..2836
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2857..2877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2881..2901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2971..2992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3232..3251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3652..3708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3811..4701
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    7371..7959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7424..7444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7842..7853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7962..7978)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7963..7979)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8484..8664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8690..8707)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8696..8920
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8966..9394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9053..9072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9263..9284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9403..9423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9408..9737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9588..9723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9650..9669
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9785..9832
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9851..10225
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10355..10476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10392..10411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10446..10465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10525..10541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10525..10541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10538..10560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10549..10565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10573..10579,10580..10597,10598..10603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10618..10639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10756..10773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10927..11515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11007..11026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11686..12477,12478..12546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12309..12328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12547..12651)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12726..12745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gagagggccc tgcgtatgag tgcaagtggg
     2401 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     2461 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     2521 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     2581 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     2641 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     2701 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     2761 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     2821 gtgcctgaga gcgcagtcgg atccgccacc atggctccaa aaaagaagag aaaggtagat
     2881 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatggt gagcaagggc
     2941 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3001 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3061 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3121 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3181 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3241 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3301 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3361 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3421 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3541 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg agctactaac
     3661 ttcagcctgc tgaagcaagc aggagatgtt gaagaaaacc ccgggcctgc tagcatgcaa
     3721 aagattatgc atatttctgt cctcctttct cctgttttat ggggactgat ttttggtgtc
     3781 tcttctggtg gctctggcgg aactggaggt atggcagaaa tcggtactgg ctttccattc
     3841 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     3901 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     3961 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     4021 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     4081 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     4141 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     4201 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     4261 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     4321 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     4381 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     4441 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     4501 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     4561 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     4621 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     4681 tcgacgctcg agatttccgg cggcactgga ggtagcggtg gtagcggcgg taacagcata
     4741 cagatagggg ggctatttcc aaggggcgct gatcaagaat acagtgcatt tcgggtaggg
     4801 atggttcagt tttccacttc ggagttcaga ctgacacccc atatcgacaa tttggaggta
     4861 gccaacagtt tcgcagtcac caatgctttc tgctcccagt tttcaagagg agtctacgca
     4921 atttttggat tttatgacaa gaagtctgta aataccatca catcattctg tgggacactc
     4981 catgtgtcct tcatcacacc tagcttccca acagatggca cacatccatt tgtcatccag
     5041 atgcgacctg acctcaaagg agcactcctt agcttgattg agtactacca atgggacaag
     5101 ttcgcatacc tctatgacag tgacagaggc ttatcaacac tgcaagctgt tctggattct
     5161 gctgcagaga agaagtggca ggtgactgct atcaatgtgg ggaacatcaa caatgacaag
     5221 aaagatgaga cctacagatc gctctttcaa gatctggagt taaaaaaaga acggcgtgta
     5281 atcctggact gtgaaaggga taaagtaaat gacattgtgg accaggttat taccattgga
     5341 aaacatgtta aagggtacca ttatatcatt gcaaatctgg gattcactga tggggacctg
     5401 ctgaaaattc agtttggagg agcaaatgtc tctggatttc agattgtaga ctacgatgat
     5461 tccctggtgt ctaaatttat agaaagatgg tcaacactgg aagagaaaga ataccctgga
     5521 gcacacacag cgacaattaa gtatacttcg gccctgacgt atgatgctgt ccaagtgatg
     5581 actgaagcat tccgtaacct tcggaagcag aggattgaaa tatcccggag aggaaatgca
     5641 ggggattgtt tggccaaccc agctgtgccc tggggacaag gggtcgaaat agaaagggcc
     5701 ctcaagcagg ttcaagttga aggcctctct ggaaatataa agtttgacca gaatggaaaa
     5761 cgaataaact acacaattaa catcatggag ctcaaaacaa atggaccccg gaagattggg
     5821 tactggagtg aagtggataa aatggttgtc accctaactg agctcccatc aggaaatgac
     5881 acgtctgggc ttgaaaacaa gactgtggtg gtcaccacaa tattggaatc tccatatgtt
     5941 atgatgaaga aaaatcatga aatgcttgaa gggaatgagc gttacgaggg ctactgtgtt
     6001 gacttagctg cagaaattgc caaacactgt gggttcaagt acaagctgac tattgttggg
     6061 gatggcaagt atggggccag ggatgccgac accaaaattt ggaatggtat ggttggagag
     6121 cttgtctacg ggaaagctga cattgcaatt gctccattaa ctatcactct cgtgagagaa
     6181 gaggtgattg acttctccaa gcccttcatg agtcttggaa tctctatcat gatcaagaag
     6241 cctcagaagt ccaaaccagg agtgttttcc tttcttgatc ctttagccta tgagatctgg
     6301 atgtgcattg tgtttgccta cattggggtc agtgtagttt tattcctggt cagcagattt
     6361 agcccctacg agtggcacac tgaggaattt gaagatggaa gagaaacaca aagtagtgaa
     6421 tcaactaatg aatttgggat ttttaatagt ctctggtttt ccttgggtgc ctttatgcgg
     6481 caaggatgcg atatttcgcc aagatccctc tctgggcgca ttgttggagg tgtgtggtgg
     6541 ttctttaccc tgatcataat ctcctcctac acggctaact tagctgcctt cctgactgta
     6601 gagaggatgg tgtctcccat cgaaagtgct gaggatctgt ctaagcaaac agaaattgct
     6661 tatggaacat tagactctgg ctccactaaa gagtttttca ggagatctaa aatcgcagtg
     6721 tttgataaaa tgtggactta tatgaggagt gcagagccct ctgtgtttgt gaggactacc
     6781 gcagaaggag tagccagagt ccggaaatcc aaaggaaagt atgcctactt gctggagtcc
     6841 acaatgaacg agtacatcga gcagaggaag ccttgtgaca ccatgaaagt gggaggaaac
     6901 ttggattcca aaggctacgg catcgccaca cctaaaggat cctcattagg aaccccagta
     6961 aatcttgcag tattgaaact cagtgagcaa ggcgtcttag acaagctgaa aaacaaatgg
     7021 tggtacgata aaggtgaatg tggagccaag gactcgggaa gtaaggaaaa gaccagtgcc
     7081 ctcagtctga gcaacgttgc tggagtattc tacatccttg tcgggggcct tggtttggca
     7141 atgctggtgg ctttgattga gttctgttac aagtcaaggg ccgaggcgaa acgaatgaag
     7201 gtggcaaaga atccacagaa tattaaccca tcttcctcgc agaattccca gaattttgca
     7261 acttataagg aaggttacaa cgtatatggc atcgagagtg ttaaaattta gggcgcgcct
     7321 tcgaattaat taaggccgcg actctagaat tcgatatcaa gcttatcgat aatcaacctc
     7381 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7441 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7501 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7561 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7621 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7681 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7741 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7801 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7861 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7921 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     7981 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8041 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     8101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8161 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     8221 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8281 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     8341 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8401 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8461 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8521 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8581 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8641 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     8701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8941 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9361 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     9421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9661 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9721 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     9781 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     9841 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     9901 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     9961 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    10021 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    10081 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    10141 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    10201 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    10261 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10321 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    10381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    10501 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    10561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10621 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    10681 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    11641 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    11701 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    11761 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11821 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11881 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11941 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12001 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12061 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12121 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12181 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12241 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12301 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12361 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12421 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12481 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12541 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12601 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    12661 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    12721 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    12781 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    12841 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    12901 gccagatata cgcgtt
//
