LOCUS       sequence-337616-       12532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNAP tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP-P2A_SNAP_GluA2
FEATURES             Location/Qualifiers
     source          1..12532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2389..2836
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2857..2877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2881..2901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2971..2992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3232..3251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3652..3708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6987..7575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7040..7060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7458..7469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7578..7594)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7579..7595)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8100..8280
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8306..8323)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8312..8536
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8582..9010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8669..8688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8879..8900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9019..9039)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9024..9353
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9204..9339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9266..9285
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9401..9448
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9467..9841
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9971..10092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10008..10027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10062..10081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10141..10157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10141..10157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10154..10176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10165..10181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10189..10195,10196..10213,10214..10219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10234..10255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10372..10389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10543..11131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10623..10642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11302..12093,12094..12162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11925..11944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12163..12267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12342..12361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gagagggccc tgcgtatgag tgcaagtggg
     2401 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     2461 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     2521 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     2581 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     2641 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     2701 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     2761 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     2821 gtgcctgaga gcgcagtcgg atccgccacc atggctccaa aaaagaagag aaaggtagat
     2881 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatggt gagcaagggc
     2941 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3001 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3061 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3121 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3181 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3241 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3301 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3361 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3421 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3541 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg agctactaac
     3661 ttcagcctgc tgaagcaagc aggagatgtt gaagaaaacc ccgggcctgc tagcatgcaa
     3721 aagattatgc atatttctgt cctcctttct cctgttttat ggggactgat ttttggtgtc
     3781 tcttctatgg gcctgggctc catggacaag gattgtgaaa tgaaacgcac cacactggac
     3841 agccctttgg ggaagctgga gctgtctggt tgtgagcagg gtctgcacga aataattttt
     3901 ctgggcaagg ggacgtctgc agctgatgcc gtggaggtcc cagcccccgc tgcggttctc
     3961 ggaggtccgg agcccctgat gcaggctaca gcctggctga atgcctattt ccaccagccc
     4021 gaggctatcg aagagttccc cgtgccggct cttcaccatc ccgttttcca gcaagagtcg
     4081 ttcaccagac aggtgttatg gaagctgctg aaggttgtga aattcggaga agtgatttct
     4141 tactctcact tagcagccct ggcaggcaac cccgcagcca cggcagcagt gaagacggca
     4201 ctgagtggca atcctgtccc tatcctgatc ccgtgccaca gagtggtcca gggggatttg
     4261 gatgtgggcg gttacgaggg tggactggcc gtgaaggaat ggcttctggc ccatgaaggc
     4321 caccggttgg ggaagccagg cttgggaaac agcatacaga taggggggct atttccaagg
     4381 ggcgctgatc aagaatacag tgcatttcgg gtagggatgg ttcagttttc cacttcggag
     4441 ttcagactga caccccatat cgacaatttg gaggtagcca acagtttcgc agtcaccaat
     4501 gctttctgct cccagttttc aagaggagtc tacgcaattt ttggatttta tgacaagaag
     4561 tctgtaaata ccatcacatc attctgtggg acactccatg tgtccttcat cacacctagc
     4621 ttcccaacag atggcacaca tccatttgtc atccagatgc gacctgacct caaaggagca
     4681 ctccttagct tgattgagta ctaccaatgg gacaagttcg catacctcta tgacagtgac
     4741 agaggcttat caacactgca agctgttctg gattctgctg cagagaagaa gtggcaggtg
     4801 actgctatca atgtggggaa catcaacaat gacaagaaag atgagaccta cagatcgctc
     4861 tttcaagatc tggagttaaa aaaagaacgg cgtgtaatcc tggactgtga aagggataaa
     4921 gtaaatgaca ttgtggacca ggttattacc attggaaaac atgttaaagg gtaccattat
     4981 atcattgcaa atctgggatt cactgatggg gacctgctga aaattcagtt tggaggagca
     5041 aatgtctctg gatttcagat tgtagactac gatgattccc tggtgtctaa atttatagaa
     5101 agatggtcaa cactggaaga gaaagaatac cctggagcac acacagcgac aattaagtat
     5161 acttcggccc tgacgtatga tgctgtccaa gtgatgactg aagcattccg taaccttcgg
     5221 aagcagagga ttgaaatatc ccggagagga aatgcagggg attgtttggc caacccagct
     5281 gtgccctggg gacaaggggt cgaaatagaa agggccctca agcaggttca agttgaaggc
     5341 ctctctggaa atataaagtt tgaccagaat ggaaaacgaa taaactacac aattaacatc
     5401 atggagctca aaacaaatgg accccggaag attgggtact ggagtgaagt ggataaaatg
     5461 gttgtcaccc taactgagct cccatcagga aatgacacgt ctgggcttga aaacaagact
     5521 gtggtggtca ccacaatatt ggaatctcca tatgttatga tgaagaaaaa tcatgaaatg
     5581 cttgaaggga atgagcgtta cgagggctac tgtgttgact tagctgcaga aattgccaaa
     5641 cactgtgggt tcaagtacaa gctgactatt gttggggatg gcaagtatgg ggccagggat
     5701 gccgacacca aaatttggaa tggtatggtt ggagagcttg tctacgggaa agctgacatt
     5761 gcaattgctc cattaactat cactctcgtg agagaagagg tgattgactt ctccaagccc
     5821 ttcatgagtc ttggaatctc tatcatgatc aagaagcctc agaagtccaa accaggagtg
     5881 ttttcctttc ttgatccttt agcctatgag atctggatgt gcattgtgtt tgcctacatt
     5941 ggggtcagtg tagttttatt cctggtcagc agatttagcc cctacgagtg gcacactgag
     6001 gaatttgaag atggaagaga aacacaaagt agtgaatcaa ctaatgaatt tgggattttt
     6061 aatagtctct ggttttcctt gggtgccttt atgcggcaag gatgcgatat ttcgccaaga
     6121 tccctctctg ggcgcattgt tggaggtgtg tggtggttct ttaccctgat cataatctcc
     6181 tcctacacgg ctaacttagc tgccttcctg actgtagaga ggatggtgtc tcccatcgaa
     6241 agtgctgagg atctgtctaa gcaaacagaa attgcttatg gaacattaga ctctggctcc
     6301 actaaagagt ttttcaggag atctaaaatc gcagtgtttg ataaaatgtg gacttatatg
     6361 aggagtgcag agccctctgt gtttgtgagg actaccgcag aaggagtagc cagagtccgg
     6421 aaatccaaag gaaagtatgc ctacttgctg gagtccacaa tgaacgagta catcgagcag
     6481 aggaagcctt gtgacaccat gaaagtggga ggaaacttgg attccaaagg ctacggcatc
     6541 gccacaccta aaggatcctc attaggaacc ccagtaaatc ttgcagtatt gaaactcagt
     6601 gagcaaggcg tcttagacaa gctgaaaaac aaatggtggt acgataaagg tgaatgtgga
     6661 gccaaggact cgggaagtaa ggaaaagacc agtgccctca gtctgagcaa cgttgctgga
     6721 gtattctaca tccttgtcgg gggccttggt ttggcaatgc tggtggcttt gattgagttc
     6781 tgttacaagt caagggccga ggcgaaacga atgaaggtgg caaagaatcc acagaatatt
     6841 aacccatctt cctcgcagaa ttcccagaat tttgcaactt ataaggaagg ttacaacgta
     6901 tatggcatcg agagtgttaa aatttagggc gcgccttcga attaattaag gccgcgactc
     6961 tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     7021 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7081 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7141 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7201 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7261 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7321 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7381 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7441 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7501 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7561 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7621 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7681 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7741 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7801 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7861 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7921 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7981 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     8041 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     8101 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8161 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8221 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8281 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8341 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8401 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8461 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8521 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8581 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8641 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8701 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8761 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8821 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8881 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8941 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9001 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9061 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     9121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9181 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     9241 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     9301 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9361 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     9421 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     9481 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     9541 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     9601 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     9661 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     9721 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     9781 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     9841 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9901 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9961 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10021 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10081 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    10141 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10201 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10261 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10981 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11101 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11161 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11221 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11281 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11341 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11401 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11461 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11521 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11581 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11641 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11701 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11761 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11821 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11881 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11941 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12001 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12061 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12121 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12181 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12241 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    12301 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    12361 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    12421 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    12481 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
