LOCUS       sequence-337623-       12709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNAP tagged NR1 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_SNAP_NR1
FEATURES             Location/Qualifiers
     source          1..12709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2389..2836
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2845..2854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2857..2877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2881..2901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2926..2928,2929..2931,2932..3642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2971..2992)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3232..3251)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3579..3600
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3652..3708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7164..7752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7217..7237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7635..7646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7755..7771)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7756..7772)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8277..8457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8483..8500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8489..8713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8759..9187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8846..8865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9056..9077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9196..9216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9201..9530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9381..9516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9443..9462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9578..9625
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9644..10018
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10148..10269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10185..10204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10239..10258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10318..10334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10318..10334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10331..10353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10342..10358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10366..10372,10373..10390,10391..10396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10411..10432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10549..10566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10720..11308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10800..10819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11479..12270,12271..12339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12102..12121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12340..12444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12519..12538)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctgtt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gagagggccc tgcgtatgag tgcaagtggg
     2401 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     2461 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     2521 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     2581 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     2641 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     2701 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     2761 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     2821 gtgcctgaga gcgcagtcgg atccgccacc atggctccaa aaaagaagag aaaggtagat
     2881 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatggt gagcaagggc
     2941 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3001 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3061 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3121 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3181 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3241 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3301 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3361 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3421 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3481 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3541 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3601 accgccgccg ggatcactct cggcatggac gagctgtaca agggatccgg agctactaac
     3661 ttcagcctgc tgaagcaagc aggagatgtt gaagaaaacc ccgggcctgc tagcatgagc
     3721 accatgcacc tgctgacatt cgccctgctt ttttcctgct ccttcgcccg cgccgccact
     3781 aggatgggcc tgggctccat ggacaaggat tgtgaaatga aacgcaccac actggacagc
     3841 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
     3901 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
     3961 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
     4021 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
     4081 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
     4141 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
     4201 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
     4261 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
     4321 cggttgggga agccaggctt gggagctagt tgcgacccca agatcgtcaa catcggcgcg
     4381 gtgctgagca cgcgcaagca tgaacagatg ttccgcgagg cagtaaacca ggccaataag
     4441 cgacacggct cttggaagat acagctcaac gccacttctg tcacccacaa gcccaacgcc
     4501 atacagatgg ccctgtcagt gtgtgaggac ctcatctcta gccaggtcta cgctatccta
     4561 gttagccacc cgcctactcc caacgaccac ttcactccca cccctgtctc ctacacagct
     4621 ggcttctaca gaatccctgt cctgggactg actacccgaa tgtccatcta ctctgacaag
     4681 agtatccacc tgagtttcct tcgcacggtg ccgccctact cccaccagtc cagcgtctgg
     4741 tttgagatga tgcgagtcta caactggaac cacatcatcc tgctggtcag cgacgaccac
     4801 gagggacggg cagcgcagaa gcgcttggag acgttgctgg aggaacggga gtccaaggca
     4861 gagaaggtgc tgcagtttga cccaggaacc aagaatgtga cggctctgct gatggaggcc
     4921 cgggaactgg aggcccgggt catcatcctt tctgcaagcg aggacgacgc tgccacagtg
     4981 taccgcgcag ccgcaatgct gaacatgacg ggctctgggt acgtgtggct ggtcggggaa
     5041 cgcgagatct ctgggaacgc cctgcgctac gctcctgatg gcatcatcgg acttcagctc
     5101 atcaatggca agaatgagtc agcccacatc agtgacgccg tgggcgtggt ggcacaggca
     5161 gttcacgaac tcctagagaa ggagaatatc actgacccac cgcggggttg cgtgggcaac
     5221 accaacatct ggaagacagg accattgttc aagagggtgc tgatgtcttc taagtatgcg
     5281 gacggagtga ctggccgtgt ggaattcaat gaggatgggg accggaagtt tgccaactat
     5341 agtatcatga acctgcagaa ccgcaagctg gtgcaagtgg gcatctacaa tggtacccat
     5401 gtcatcccaa atgacaggaa gatcatctgg ccaggaggag agacagagaa acctcgagga
     5461 taccagatgt ccaccagact aaagatagtg acaatccacc aagagccctt cgtgtacgtc
     5521 aagcccacaa tgagtgatgg gacatgcaaa gaggagttca cagtcaatgg tgacccagtg
     5581 aagaaggtga tctgtacggg gcctaatgac acgtccccag gcagcccacg ccacacagtg
     5641 ccccagtgct gctatggctt ctgcatagac ctgctcatca agctggcgcg gaccatgaat
     5701 tttacctatg aggtgcacct ggtggcagat ggcaagtttg gcacacagga gcgggtaaac
     5761 aacagcaaca aaaaggagtg gaacggaatg atgggcgagc tactcagtgg ccaagcggac
     5821 atgattgtgg caccactgac catcaacaat gagcgtgcgc agtacataga gttctccaag
     5881 cccttcaagt accagggcct gaccattttg gtcaagaagg agattcccag gagcacactg
     5941 gactcattta tgcagccttt tcagagcaca ctgtggttgc tagtaggact gtcagttcat
     6001 gtggtggctg tgatgctgta cctgctggac cgcttcagtc cctttggccg attcaaggtg
     6061 aacagtgagg aggaggagga agatgcactg accctgtcct ctgccatgtg gttttcctgg
     6121 ggcgtcctgc tcaactccgg cattggggaa ggtgcccccc ggagtttctc tgcacgtatc
     6181 ctaggcatgg tgtgggctgg tttcgccatg atcatagtgg cttcctacac tgccaacttg
     6241 gcagctttcc tggtgctgga tcggcctgag gagcgcatca cgggcatcaa tgaccccagg
     6301 ctcagaaacc cctcagacaa gttcatctac gcaactgtaa agcagagctc cgtggacatc
     6361 tacttccgga ggcaggtgga gttgagtacc atgtaccggc acatggaaaa acacaattac
     6421 gagagcgcag ctgaggccat ccaggctgtg cgggacaaca agctgcacgc ctttatctgg
     6481 gactcggccg tgctggagtt tgaggcttca cagaagtgcg atctggtgac cacgggtgag
     6541 ctgttcttcc gctcaggctt tggcatcggc atgcgcaagg acagcccctg gaagcagaac
     6601 gtttccctgt ccatactcaa gtcccatgag aatggcttca tggaagatct ggataagaca
     6661 tgggttcggt atcaggaatg cgactcccgc agcaatgctc ctgcaaccct cacttttgag
     6721 aacatggcag gggtcttcat gctggtggct ggaggcatcg tagctgggat tttcctcatt
     6781 ttcattgaga tcgcctacaa gcgacacaag gatgcccgta ggaagcagat gcagctggct
     6841 tttgcagccg tgaacgtgtg gaggaagaac ctgcaggata gaaagagtgg tagagcagag
     6901 cccgacccta aaaagaaagc cacatttagg gctatcacct ccaccctggc ctccagcttc
     6961 aagagacgta ggtcctccaa agacacgagc accgggggtg gacgcggcgc tttgcaaaac
     7021 caaaaagaca cagtgctgcc gcgacgcgct attgagaggg aggagggcca gctgcagctg
     7081 tgttcccgtc atagggagag ctgaggcgcg ccttcgaatt aattaaggcc gcgactctag
     7141 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     7201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7741 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     7801 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7861 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     7921 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7981 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     8041 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     8101 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     8161 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     8221 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     8281 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8341 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8401 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     8461 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8521 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8581 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8641 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8701 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     8761 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8821 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8881 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8941 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9001 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9061 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9121 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9181 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9361 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9421 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     9481 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     9541 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     9601 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     9661 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     9721 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     9781 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     9841 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     9901 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     9961 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    10021 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    10081 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    10141 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10201 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10261 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    10321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    10561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    11041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    11101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    12481 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    12541 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    12601 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    12661 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
