LOCUS       sequence-337631-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replacing the RSV promoter of pLKO.1 with the CMV promoter improves 
            lentiviral production with third-generation viral packaging system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Lee S, Cobrinik D
  TITLE     Improved third-generation lentiviral packaging with pLKO.1C vectors.
  JOURNAL   Biotechniques. 2020 Jun;68(6):349-352. doi: 10.2144/btn-2019-0155. 
            Epub 2020 Mar 6.
  PUBMED    32141762
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.2144/btn-2019-0155"; journalName: "Biotechniques"; date: 
            "2020-06"; volume: "68"; issue: "6"; pages: "349-352"
COMMENT     Sequence Label: pLKO.1C-YFP
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..96
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(175..177,178..180,181..894)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(220..241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(481..500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     828..849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             961..1194
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1266..1387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1303..1322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1357..1376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1427..1562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1582..1601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1583..1601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1611..1628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1611..1627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1620..1642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1769..2224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1856..1875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2066..2087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2250..2354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2355..2423,2424..3215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2573..2592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3386..3974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3875..3894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4128..4145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4262..4283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4298..4303,4304..4321,4322..4328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4336..4352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4341..4363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4360..4376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4360..4376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4395..4415
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4397..4415
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        4516..4895
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4896..5099
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5050..5070
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5114..5294
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5341..5466
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5959..6192
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6377..6421
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6719..6967
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6719..6739
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6890..6909
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    8881..8998
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9047..9398,1..159)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
       61 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
      121 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatctcgc caccatggtg
      181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      361 accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      601 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      661 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      781 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      841 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaactttaa
      901 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
      961 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     1021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     1081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     1201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     1261 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1381 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     1441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1561 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     1621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1741 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     1801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1981 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2101 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2221 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4381 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     4441 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
     4501 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4561 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4621 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4681 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4741 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4801 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4861 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     4921 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4981 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     5041 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     5101 gtttagtgaa ccggggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5281 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     5341 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     5401 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     5461 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     5521 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     5581 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     5641 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     5701 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     5761 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     5821 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5881 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5941 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6001 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6061 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6121 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     6181 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     6241 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     6301 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     6361 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     6421 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     6481 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     6541 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     6601 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     6661 gaacggatct cgacggtatc gatcacgaga ctagcctcga gcggccgccc ccttcaccga
     6721 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     6781 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     6841 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     6901 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     6961 aaacaccggt gtgttgtaaa tgagcacaca aaatacacat gctaaaatat tatattctat
     7021 gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa taattagaat
     7081 ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa acgctagttt
     7141 tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact tgttcctaag
     7201 cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac tccaccagct
     7261 gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc ttcaaatgtt
     7321 gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct
     7381 atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat aatacattta
     7441 acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac taattcataa
     7501 tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc accatcttta
     7561 attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag aggaaaacct
     7621 acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata gcttgttcaa
     7681 tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca taattgttta
     7741 atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt gtttagtttc
     7801 attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg tctactttaa
     7861 ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt ttaggttcag
     7921 ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt agtaagtctt
     7981 gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact ttaagtccag
     8041 caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt tttaatttct
     8101 tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc atttctttaa
     8161 ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat caagcagcct
     8221 ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca tcaattacat
     8281 atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct tctttagcaa
     8341 tttctctacc cattcattta aatccagtta aagtttttac aatattaact ccatattttt
     8401 catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga gccggatttt
     8461 ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa attggtcctg
     8521 tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt ccaaatctgt
     8581 cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca tattcaagcg
     8641 gtatttttca tgcaggatca aattctggat ttggatttac aacattttta aatgtttcat
     8701 cttcaaatgc atgctcttca acctcaataa cgttatatcc tgattcacgt aatatttttg
     8761 gggtaaattt agttcctgtt ccattaactg cgctaaaaat aatttttaaa tcttttttag
     8821 cttcttgctc ttttttgtag aattctcgac ctcgagacaa atggcagtat tcatccacaa
     8881 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8941 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9001 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     9061 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     9121 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     9181 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     9241 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     9301 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     9361 gggctgtggc caatagcggc tgctcagcag ggcgcgcc
//
