LOCUS       sequence-337635-        9289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the purifcation of human ULK1 kinase dead complex .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Chang C, Shi X, Jensen LE, Yokom AL, Fracchiolla D, Martens S, 
            Hurley JH
  TITLE     Reconstitution of cargo-induced LC3 lipidation in mammalian 
            selective autophagy.
  JOURNAL   Sci Adv. 2021 Apr 23;7(17). pii: 7/17/eabg4922. doi: 
            10.1126/sciadv.abg4922. Print 2021 Apr.
  PUBMED    33893090
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-04-23"; volume: "7(17). pii: 7/17/eabg4922. doi"; pages:
            " 10.1126/sciadv.abg4922. Print 2021 Apr"
COMMENT     Sequence Label: MBP-TSF-TEVcs-ULK1K46I
FEATURES             Location/Qualifiers
     source          1..9289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1754..1763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1769..2869
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2843..2866
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2888..2911
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2948..2971
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2978..3001
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3008..3028
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(6311..6330)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6376..6431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6430..6449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6792..6808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6792..6808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6805..6827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6816..6832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6840..6846,6847..6864,6865..6870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6885..6906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6965..7160
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7011..7146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7166..7300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7203..7222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7257..7276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7368..7385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7539..8127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7619..7638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8298..9089,9090..9158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8921..8940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9159..9263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gcgccrggsg gggsggggsg gggsgrgggg
      541 sggggsgggg sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgat
     1741 cgacggtatc gatgccacca tggctagcat gaaaatcgaa gaaggtaaac tggtaatctg
     1801 gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga
     1861 taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt
     1921 tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta
     1981 cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta
     2041 tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt
     2101 tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga
     2161 agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa
     2221 cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa
     2281 gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc
     2341 gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta
     2401 ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg
     2461 ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt
     2521 caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag
     2581 tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct
     2641 ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga
     2701 gttggcgaaa gatccacgta ttgccgccac tatggaaaac gcccagaaag gtgaaatcat
     2761 gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc
     2821 cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagacta attcgagctc
     2881 ggctagctgg agccacccgc agttcgagaa aggtggaggt gcccgaggtg gatcgggagg
     2941 tggatcgtgg agccacccgc agttcgaaaa aggattcgat tacaaggatg acgatgacaa
     3001 gggtaccgaa aacctgtact tccaatccac cggtggtccc gggatggaac ctggcagagg
     3061 aggaacagaa accgtcggaa aattcgagtt tagcagaaag gacctgattg gacatggagc
     3121 attcgcagtc gtgttcaagg gtcgtcaccg ggaaaaacat gacctggagg tggccgtcat
     3181 ctgcatcaac aagaaaaatc tggctaaatc ccagaccctg ctcggcaagg aaatcaaaat
     3241 tctgaaggag ctcaaacacg aaaacattgt ggccctgtac gacttccagg aaatggctaa
     3301 ctctgtgtac ctggtcatgg agtattgcaa tggcggagac ctggctgatt atctccatgc
     3361 aatgagaacc ctgagcgagg acactatccg tctgttcctc cagcagattg ctggtgcaat
     3421 gcgtctgctc cactccaagg gcatcattca tcgggatctg aaaccccaga acatcctgct
     3481 ctccaatcct gctggccgca gggcaaaccc aaattctatc agggtgaaga ttgccgactt
     3541 cggatttgct agatacctgc agtctaacat gatggccgct acactgtgcg gttcacctat
     3601 gtacatggct ccagaggtca ttatgtcaca gcactatgac ggaaaggccg atctgtggag
     3661 tatcggtact attgtgtacc agtgtctgac aggcaaagcc cctttccagg ctagctcccc
     3721 acaggacctg cgcctctttt atgaaaagaa caaaaccctg gtgccaacta tcccccgtga
     3781 gacctctgca cctctgcgtc agctgctcct ggcactcctg cagagaaatc acaaggaccg
     3841 tatggacttc gatgagttct ttcaccatcc ctttctggat gcttcaccta gtgtgaggaa
     3901 atccccccct gtgcctgtcc caagctaccc atcttcaggt tccggcagta gctcctcttc
     3961 aagtagcacc tctcacctgg catccccacc atctctcgga gagatgcagc agctgcagaa
     4021 gaccctcgcc tcacccgctg acactgcagg attcctgcat tcctctagag attcaggtgg
     4081 cagtaaagac tcaagttgcg atactgacga tttcgtgatg gtccccgctc agtttcctgg
     4141 agacctggtc gccgaagctc ctagcgccaa gcctccaccc gattccctga tgtgttctgg
     4201 tagctccctg gtggcatctg caggtctcga gtcacacggt cgtaccccaa gcccttcccc
     4261 tccatgctct tcaagtccat ctccctcagg acgggcaggt ccattcagct cctctcgctg
     4321 tggcgcctct gtgcctatcc cagtccccac acaggtgcag aactaccagc ggattgaacg
     4381 caatctgcag agcccaacac agttccagac cccccgttca agtgctatca gacgtagtgg
     4441 cagcacctcc ccactgggat ttgctcgtgc atctccatca ccacctgcac acgctgagca
     4501 tggaggtgtc ctggccagga agatgagtct cggcggaggt agaccctata cacctagccc
     4561 acaagtgggt accattcctg aaaggccagg ctggtcagga acccccagtc ctcagggtgc
     4621 tgagatgagg ggcggaagat ccccacgtcc aggtagctcc gcaccagagc acagcccacg
     4681 tacttccgga ctgggttgca ggctccacag tgctcctaac ctgagcgacc tccatgtggt
     4741 ccgtcccaag ctgcctaaac cacccaccga tcccctggga gcagtgttct cccctccaca
     4801 ggcatctcca cctcagcctt cacatggact gcagagttgt cggaacctcc gcggtagccc
     4861 aaagctgccc gacttcctcc agagaaatcc cctgccaccc atcctcggat cccccacaaa
     4921 ggccgtccct tctttcgatt ttcctaaaac cccatcttca cagaacctcc tggccctcct
     4981 ggctcgtcag ggtgtggtca tgacacctcc acgcaatagg accctgccag acctctccga
     5041 agtgggtcct ttccacggac agccactggg tcctggactc cgtccaggcg aggatccaaa
     5101 gggtcccttc ggccggagtt ttagcacttc ccgcctgaca gacctcctgc tcaaagcagc
     5161 cttcggtaca caggcccctg atccaggcag caccgagtct ctgcaggaga agccaatgga
     5221 gatcgcaccc tctgccggct ttggtggttc actgcaccca ggagcaaggg caggaggtac
     5281 cagtagcccc tcacctgtgg tcttcactgt gggaagtccc ccttctggtt caactccacc
     5341 ccagggccct agaacacgta tgtttagtgc tggaccaact ggtagcgcat cctcttcagc
     5401 cagacacctg gtcccaggac cctgcagcga agctcctgca ccagagctgc cagcacctgg
     5461 tcatggttgt tccttcgcag accctatcac tgccaacctg gaaggcgctg tgacatttga
     5521 ggcacctgat ctgccagagg aaaccctcat ggagcaggaa cacactgaga tcctgagggg
     5581 tctcagattc accctgctct ttgtgcagca tgtcctggaa attgctgcac tcaagggtag
     5641 tgctagcgag gcagctggtg gaccagaata ccagctgcag gagagcgtgg tcgccgacca
     5701 gatctctctg ctctcaaggg aatggggctt cgctgagcag ctggtcctct atctgaaggt
     5761 ggccgaactg ctcagtagcg gcctgcagtc cgccatcgat cagattagag ctggaaagct
     5821 ctgcctgtcc tctactgtga aacaggtggt ccggcgcctg aacgagctct acaaggccag
     5881 tgtggtcagc tgtcagggac tctctctgcg tctccagcgg ttctttctgg acaaacagcg
     5941 cctgctcgat aggatccact caattacagc tgaacgcctg atcttcagcc atgcagtgca
     6001 gatggtccag tccgcagccc tggacgaaat gtttcagcac agggagggat gcgtccccag
     6061 ataccataag gctctgctcc tgctcgaggg tctgcagcac atgctcagcg accaggccga
     6121 tatcgaaaat gtgactaagt gcaaactgtg tattgagagg agactctccg ctctgctcac
     6181 aggcatctgt gcataacccg ggtgatatct taattaatta actcgaggaa ttcactcctc
     6241 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     6301 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     6361 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     6421 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     6481 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     6541 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     6601 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     6661 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     6721 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     6781 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6841 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6901 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     6961 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7081 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7141 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     7201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7261 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     7321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
