LOCUS       sequence-337636-       14041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / 
            UK variant) with N-terminal c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, 
            Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, 
            Rehman J, Malik AB
  TITLE     Engineered ACE2 decoy mitigates lung injury and death induced by 
            SARS-CoV-2 variants.
  JOURNAL   Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 
            10.1038/s41589-021-00965-6. Epub 2022 Jan 19.
  PUBMED    35046611
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00965-6"; journalName: "Nat Chem Biol"; date: 
            "2022-03"; volume: "18"; issue: "3"; pages: "342-351"
COMMENT     Sequence Label: pCEP4-myc-S (B.1.1.7 lineage / UK variant)
FEATURES             Location/Qualifiers
     source          1..14041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        289..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        669..872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     823..843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             975..1004
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4810..4944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4834..4853)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4888..4907
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5087..5109)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5669..7458
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(7760..9685)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGGGXGGGX
                     GGXGGXGXGGGGGGGGGGGGGGGGGGGGGGGGXGGGGGXGXGGXGGXGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGXGGXGGXGXGGGXGGXGAG
                     GGAGGXGAGGXGGXGXGGXGGXGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10120..10138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10206..10310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10311..10379,10380..11171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10529..10548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11342..11930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11831..11850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12361..12506
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             12549..13586
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(13584..13603)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13628..13676
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
       61 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
      121 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
      181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atctctagaa
      901 gctgggtacc agctgctagc gccaccatga agaccatcat cgccctgagc tacatcttct
      961 gcctggtgtt cgccgagcaa aagctgatct cagaggagga cctgggcagc cctggcggtg
     1021 ccgtcaatct gacaactcgg actcagctgc cacctgctta tactaatagc ttcaccagag
     1081 gcgtgtacta tcctgacaag gtgtttagaa gctccgtgct gcactctaca caggatctgt
     1141 ttctgccatt ctttagcaac gtgacctggt tccacgccat cagcggcacc aatggcacaa
     1201 agcggttcga caatcccgtg ctgcctttta acgatggcgt gtacttcgcc tctaccgaga
     1261 agagcaacat catcagaggc tggatctttg gcaccacact ggactccaag acacagtctc
     1321 tgctgatcgt gaacaatgcc accaacgtgg tcatcaaggt gtgcgagttc cagttttgta
     1381 atgatccctt cctgggcgtg tatcacaaga acaataagag ctggatggag tccgagttta
     1441 gagtgtattc tagcgccaac aactgcacat ttgagtacgt gagccagcct ttcctgatgg
     1501 acctggaggg caagcagggc aatttcaaga acctgaggga gttcgtgttt aagaatatcg
     1561 acggctactt caaaatctac tctaagcaca cccccatcaa cctggtgcgc gacctgcctc
     1621 agggcttcag cgccctggag cccctggtgg atctgcctat cggcatcaac atcacccggt
     1681 ttcagacact gctggccctg cacagaagct acctgacacc cggcgactcc tctagcggat
     1741 ggaccgccgg cgctgccgcc tactatgtgg gctacctcca gccccggacc ttcctgctga
     1801 agtacaacga gaatggcacc atcacagacg cagtggattg cgccctggac cccctgagcg
     1861 agacaaagtg tacactgaag tcctttaccg tggagaaggg catctatcag acatccaatt
     1921 tcagggtgca gccaaccgag tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat
     1981 ttggcgaggt gttcaacgca acccgcttcg ccagcgtgta cgcctggaat aggaagcgga
     2041 tcagcaactg cgtggccgac tatagcgtgc tgtacaactc cgcctctttc agcaccttta
     2101 agtgctatgg cgtgtccccc acaaagctga atgacctgtg ctttaccaac gtctacgccg
     2161 attctttcgt gatcaggggc gacgaggtgc gccagatcgc ccccggccag acaggcaaga
     2221 tcgcagacta caattataag ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca
     2281 gcaacaatct ggattccaaa gtgggcggca actacaatta tctgtaccgg ctgtttagaa
     2341 agagcaatct gaagcccttc gagagggaca tctctacaga aatctaccag gccggcagca
     2401 ccccttgcaa tggcgtggag ggctttaact gttatttccc actccagtcc tacggcttcc
     2461 agcccacata cggcgtgggc tatcagcctt accgcgtggt ggtgctgagc tttgagctgc
     2521 tgcacgcccc agcaacagtg tgcggcccca agaagtccac caatctggtg aagaacaagt
     2581 gcgtgaactt caacttcaac ggcctgaccg gcacaggcgt gctgaccgag tccaacaaga
     2641 agttcctgcc atttcagcag ttcggcaggg acatcgacga taccacagac gccgtgcgcg
     2701 acccacagac cctggagatc ctggacatca caccctgctc tttcggcggc gtgagcgtga
     2761 tcacacccgg caccaataca agcaaccagg tggccgtgct gtatcagggc gtgaattgta
     2821 ccgaggtgcc cgtggctatc cacgccgatc agctgacccc aacatggcgg gtgtacagca
     2881 ccggctccaa cgtcttccag acaagagccg gatgcctgat cggagcagag cacgtgaaca
     2941 attcctatga gtgcgacatc ccaatcggcg ccggcatctg tgcctcttac cagacccaga
     3001 caaactctca cagaagagcc cggagcgtgg cctcccagtc tatcatcgcc tataccatgt
     3061 ccctgggcgc cgagaacagc gtggcctact ctaacaatag catcgccatc ccaatcaact
     3121 tcacaatctc tgtgaccaca gagatcctgc ccgtgtccat gaccaagaca tctgtggact
     3181 gcacaatgta tatctgtggc gattctaccg agtgcagcaa cctgctgctc cagtacggca
     3241 gcttttgtac ccagctgaat agagccctga caggcatcgc cgtggagcag gataagaaca
     3301 cacaggaggt gttcgcccag gtgaagcaaa tctacaagac cccccctatc aaggactttg
     3361 gcggcttcaa tttttcccag atcctgcctg atccatccaa gccttctaag cggagcttta
     3421 tcgaggacct gctgttcaac aaggtgaccc tggccgatgc cggcttcatc aagcagtatg
     3481 gcgattgcct gggcgacatc gcagccaggg acctgatctg cgcccagaag tttaatggcc
     3541 tgaccgtgct gccacccctg ctgacagatg agatgatcgc acagtacaca agcgccctgc
     3601 tggccggcac catcacatcc ggatggacct tcggcgcagg agccgccctc cagatcccct
     3661 ttgccatgca gatggcctat aggttcaacg gcatcggcgt gacccagaat gtgctgtacg
     3721 agaaccagaa gctgatcgcc aatcagttta actccgccat cggcaagatc caggacagcc
     3781 tgtcctctac agccagcgcc ctgggcaagc tccaggatgt ggtgaatcag aacgcccagg
     3841 ccctgaatac cctggtgaag cagctgagca gcaacttcgg cgccatctct agcgtgctga
     3901 atgacatcct ggcccggctg gacaaggtgg aggcagaggt gcagatcgac cggctgatca
     3961 ccggccggct ccagagcctc cagacctatg tgacacagca gctgatcagg gccgccgaga
     4021 tcagggccag cgccaatctg gcagcaacca agatgtccga gtgcgtgctg ggccagtcta
     4081 agagagtgga cttttgtggc aagggctatc acctgatgtc cttccctcag tctgccccac
     4141 acggcgtggt gtttctgcac gtgacctacg tgcccgccca ggagaagaac ttcaccacag
     4201 cccctgccat ctgccacgat ggcaaggccc actttccaag ggagggcgtg ttcgtgtcca
     4261 acggcaccca ctggtttgtg acacagcgca atttctacga gccccagatc atcaccacac
     4321 acaacacctt cgtgagcggc aactgtgacg tggtcatcgg catcgtgaac aataccgtgt
     4381 atgatccact ccagcccgag ctggacagct ttaaggagga gctggataag tatttcaaga
     4441 atcacacctc ccctgacgtg gatctgggcg acatcagcgg catcaatgcc tccgtggtga
     4501 acatccagaa ggagatcgac cgcctgaacg aggtggctaa gaatctgaac gagagcctga
     4561 tcgacctcca ggagctgggc aagtatgagc agtacatcaa gtggccctgg tacatctggc
     4621 tgggcttcat cgccggcctg atcgccatcg tgatggtgac catcatgctg tgctgtatga
     4681 catcctgctg ttcttgcctg aagggctgct gtagctgtgg ctcctgctgt aagtttgacg
     4741 aggatgactc tgaacctgtg ctgaagggcg tgaagctgca ttacacctaa ctcgaggccg
     4801 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4861 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4921 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4981 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     5041 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     5101 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     5161 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     5221 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     5281 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     5341 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     5401 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     5461 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     5521 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     5581 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     5641 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     5701 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     5761 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     5821 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     5881 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     5941 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     6001 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     6061 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     6121 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     6181 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     6241 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     6301 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     6361 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     6421 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     6481 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     6541 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     6601 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     6661 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     6721 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     6781 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     6841 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     6901 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     6961 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     7021 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     7081 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     7141 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     7201 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     7261 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     7321 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
     7381 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
     7441 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
     7501 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
     7561 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
     7621 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
     7681 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
     7741 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
     7801 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
     7861 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
     7921 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
     7981 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
     8041 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
     8101 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
     8161 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
     8221 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
     8281 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
     8341 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
     8401 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
     8461 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
     8521 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
     8581 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
     8641 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
     8701 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
     8761 ctgcccctcc tgctcctscc cctcctsccc ctcctsctcc tscccctcct scccctcctg
     8821 ctcctscccc tcctgcccct cctcctgctc ctscccctcc tscccctcct cctsctccts
     8881 cccctcctsc ccctcctsct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     8941 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     9001 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctsccc
     9061 ctcctscccc tcctsctcct scccctcctc ctcctcctsc ccctcctccy cctcctcccc
     9121 ctcctcctcc tcctccycct cctccccctc ctcctcctcc tccycctcct cctsctccts
     9181 cccctcctsc ccctcctscc cctcctccts ctcctccccc tcctscccct cctcctsctc
     9241 ctscycctcc tcctsctcct scccctccts cccctcctsc ccctcctcct sctcctsccc
     9301 ctcctcctsc tcctscccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     9361 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
     9421 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
     9481 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
     9541 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
     9601 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
     9661 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
     9721 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
     9781 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
     9841 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
     9901 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
     9961 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
    10021 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
    10081 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
    10141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    10201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
    10561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
    10861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11461 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    11521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11941 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
    12001 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
    12061 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
    12121 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
    12181 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
    12241 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
    12301 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
    12361 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
    12421 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
    12481 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
    12541 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    12601 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    12661 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    12721 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    12781 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    12841 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    12901 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    12961 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    13021 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    13081 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    13141 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    13201 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    13261 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    13321 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    13381 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    13441 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    13501 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
    13561 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    13621 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
    13681 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    13741 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    13801 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
    13861 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
    13921 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
    13981 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
    14041 c
//
