LOCUS       sequence-337643-        2767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - AtACT2t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+AtAct2t
FEATURES             Location/Qualifiers
     source          1..2767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    436..457
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      460..503
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(507..525)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             687..1346
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(753..772)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1367..1461
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1644..2232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2133..2152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2386..2403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2427..2484)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 cgctttaact attcagaata tcagttccaa gtgaagatgg aggtagcaaa agtaagacta
       61 ataaccaaat caataaaaat cttatacaat acttatatta acattgcaaa gagtttcaag
      121 gtttttcttc tgagttttga aagaaattat agggaacaaa aggaataaag aggcatcaat
      181 tcgatcactc agagctacaa aacaatggga ctaaaacgca aaacgaaagc ggtttttttt
      241 tttgaaacgc agacgtaagt aaaaacccag agagtttgtc acacacaagt gcatcataga
      301 aacgaaaaca aaaagggaaa tgaaacaaac aaatggagaa gcaaatataa aagaaaaaaa
      361 gaaactttga tcccattcat aaaaccccag ctttttaagc ctttgatctt gagagcaagc
      421 cgagaccact catcgctcta gaagcggccg cgaattccag aaatcatcct tagcgaaagc
      481 taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt
      541 catgataata atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag
      601 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
      661 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      721 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      781 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      841 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      901 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      961 acaccgtttt ccatgagcaa actgaaacgt tttcatcact ctggagtgaa taccacgacg
     1021 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1081 cctatttccc taaagggttt attgagaata tgtttttcgt atcagccaat ccctgggtga
     1141 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1201 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1261 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1321 gcgacgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata
     1381 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1441 gcgtttttta ttggtgagaa tccaagcctc gagctgtcag accaagttta ctcatatata
     1501 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1561 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2221 agcctgtgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2341 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2401 gaggaagcct gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt
     2461 gacaccttgc ccttttttgc cggactgcag cggccgctac tagtcgcgat gagtggtctc
     2521 aagcgacttt ttggaatcaa gatgtataaa ccaaatgtgc aggacaattt gaaattaatt
     2581 tgttatattt tgcatcatgt aaaaggatta ataagcgaaa agagtaacga atagatacac
     2641 ctcgtaattg ttttggtgta aaacttgaaa cagtagaatt gacgattggg gttatataaa
     2701 atggtgcgat cttaaaatat ctgactttgt gctagtttaa gttcatttgt gaatggagga
     2761 catgtaa
//
