LOCUS       sequence-337652-       16969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pGGG_Sr1644-1Sh in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Yu G, Matny O, Champouret N, Steuernagel B, Moscou MJ, 
            Hernandez-Pinzon I, Green P, Hayta S, Smedley M, Harwood W, Kangara 
            N, Yue Y, Gardener C, Banfield MJ, Olivera PD, Welchin C, Simmons J,
            Millet E, Minz-Dub A, Ronen M, Avni R, Sharon A, Patpour M, Justesen
            AF, Jayakodi M, Himmelbach A, Stein N, Wu S, Poland J, Ens J, 
            Pozniak C, Karafiatova M, Molnar I, Dolezel J, Ward ER, Reuber TL, 
            Jones JDG, Mascher M, Steffenson BJ, Wulff BBH
  TITLE     Aegilops sharonensis genome-assisted identification of stem rust 
            resistance gene Sr62.
  JOURNAL   Nat Commun. 2022 Mar 25;13(1):1607. doi: 10.1038/s41467-022-29132-8.
  PUBMED    35338132
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-03-25"; volume: "13(1):1607. doi"; pages: " 
            10.1038/s41467-022-29132-8"
COMMENT     Sequence Label: pGGG_Sr1644-1Sh
FEATURES             Location/Qualifiers
     source          1..16969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     misc_feature    640..664
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(764..1016)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          1716..1905
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             complement(2787..2804)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2807..3649)
                     /label=Act1 promoter
                     /note="rice actin promoter"
     misc_feature    14961..14985
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(15076..15664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15156..15175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15835..16650)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(15835..16650)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     16558..16577
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     16642..16661
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(16941..16969,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt tagtcatggt tgatgggctg cctgtatcga gtggtgattt
      601 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
      661 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
      721 ttgaacactc tgtgggtctc atgccgaatt cggatccagc gtcgatctag taacatagat
      781 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
      841 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
      901 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
      961 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     1021 tgacaagcct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     1081 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     1141 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     1201 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     1261 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     1321 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     1381 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     1441 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     1501 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     1561 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     1621 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     1681 gccgcagcga tcgcatccat ggcctccgcg accggctgca gttatcatca tcatcataga
     1741 cacacgaaat aaagtaatca gattatcagt taaagctatg taatatttac accataacca
     1801 atcaattaaa aaatagatca gtttaaagaa agatcaaagc tcaaaaaaat aaaaagagaa
     1861 aagggtccta accaagaaaa tgaaggagaa aaactagaaa tttacctgca gaacagcggg
     1921 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     1981 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     2041 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     2101 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     2161 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     2221 cgcggtgagt tcaggctttt tcattggctt ctacctacaa aaaagctccg cacgaggctg
     2281 catttgtcac aaatcatgaa aagaaaaact accgatgaac aatgctgagg gattcaaatt
     2341 ctacccacaa aaagaagaaa gaaagatcta gcacatctaa gcctgacgaa gcagcagaaa
     2401 tatataaaaa tataaaccat agtgcccttt tcccctcttc ctgatcttgt ttagcatggc
     2461 ggaaatttta aaccccccat catctccccc aacaacggcg gatcgcagat ctacatccga
     2521 gagccccatt ccccgcgaga tccgggccgg atccacgccg gcgagagccc cagccgcgag
     2581 atcccgcccc tcccgcgcac cgatctgggc gcgcacgaag ccgcctctcg cccacccaaa
     2641 ctaccaaggc caaagatcgt gtccgagacg gaaaaaaaaa acggagaaag aaagaggaga
     2701 ggggcggggt ggttaccggc gcggcggcgg cggaggggga ggggggagga gctcgtcgtc
     2761 cggcagcgag gggggaggag gtggaggtgg tggtggtggt ggtggtaggg ttggggggat
     2821 gggaggagag gggggggtat gtatatagtg gcgatggggg gcgtttcttt ggaagcggag
     2881 ggagggccgg cctcgtcgct ggctcgcgat cctcctcgcg tttccggccc ccacgacccg
     2941 gacccacctg ctgttttttc tttttctttt ttttctttct tttttttttt ttggctgcga
     3001 gacgtgcggt gcgtgcggac aactcacggt gatagtgggg gggtgtggag actattgtcc
     3061 agttggctgg actggggtgg gttgggttgg gttgggttgg gctgggcttg ctatggatcg
     3121 tggatagcac tttgggcttt aggaacttta ggggttgttt ttgtaaatgt tttgagtcta
     3181 agtttatctt ttatttttac tagaaaaaat acccatgcgc tgcaacgggg gaaagctatt
     3241 ttaatcttat tattgttcat tgtgagaatt cgcctgaata tatatttttc tcaaaaatta
     3301 tgtcaaatta gcatatgggt ttttttaaag atatttctta tacaaatccc tctgtattta
     3361 caaaagcaaa cgaacttaaa acccgactca aatacagata tgcatttcca aaagcgaata
     3421 aacttaaaaa ccaattcata caaaaatgac gtatcaaagt accgacaaaa acatcctcaa
     3481 tttttataat agtagaaaag agtaaatttc actttgggcc accttttatt accgatattt
     3541 tactttatac caccttttaa ctgatgtttt cacttttgac caggtaatct tacctttgtt
     3601 ttattttgga ctatcccgac tctcttctca agcatatgaa tgacctcgag tatgctagct
     3661 ccgcaagagg cggccgcagg tggtgattag ataacggtaa tgactatgat gattaaatag
     3721 tgataatgac gactacgatg ttttttagct agctagctag agtatgttga tgatatgatg
     3781 caagagtttt tatattaata tgataatgat gatgagttat tatatcattt aatgaaagaa
     3841 accatatatt agtttcagct ggatggatcc tacctaagtg atcaagtata tatatatgcc
     3901 atatccacat atattatact tgatcaccta attaggtgat caagtataat atcgatatgg
     3961 catatacttg atcacttagc tagctagatc cttcagtcga aactaatccg cggtactttc
     4021 atctaatgat ttagcaactc attataatgt aaaagaatca ctaaattaaa ttaaaaacac
     4081 aaaactaaag caaaaataaa aaaaaacacc caaacattta gtactggttg gtgttaccaa
     4141 ccggtactaa agccctacgc gcacacgggt ctggctcgtg ccacgtgttt gcactttagc
     4201 gccggtttgt gacgaaccgg tactaaaggg ggggggggct ttagtcccca ctctttagtg
     4261 ccggttggcg aaccggcact aaaggccggt tctgcactag tgactcataa acaggtgaag
     4321 cacaatccct tacaaaactg ttcttctggg tctggtactt tgggattgaa ccataccctt
     4381 tccttcaact tcatattcct tctcttccta ccacttggta gtttataggt tccttcgggc
     4441 tcatcttctt catcactaat gccataatca ctaggaaaac atttttcatc agcttcaggc
     4501 aaccaatcta gtttttccat tttctcagcc tcactgtgtg atctaattgt tggaccagga
     4561 tttctcactg ccttcagttt ttgtgccatt ggtctatctt catcatcaga ggaagaaact
     4621 gcttgcagtt ccacattgtt gccatctgaa tcagatagaa attctgatac atcagtgtca
     4681 ccttcaaaat gattgaggtc agcttctctc tcaagaatac ttttcgtaag ctcttccttt
     4741 ctgtccatgc ttgtatccac caacttagtg caactttgtt ggatgttaat gcaatgatca
     4801 accaaataat aatctccgtt ttcatctaca ttacatagct cattttccct cacaactctt
     4861 atgttcacac tcttttcatt tagagagtgg agcaacatct gctgcacatc atcttcagaa
     4921 gatattttct ctaagccttc tactcccttt atgatcatca ctaacatagt acatataatc
     4981 atcagaacaa caaccctcac tcctaataag agataccaga gtcccaaatg atatgtctga
     5041 ctggtacatg tttctaacag cagaatctct gccttcgaaa tggaaccaaa ttccccattt
     5101 tgggtcatca atactggaca aaaagggaat gaattattat aaagaatgca cattgttaaa
     5161 gttcctaaca atttgcaatt actctgttat catacatttt gggaaactga cctaaaatac
     5221 atcctgtcaa taaaataaaa ctgaaaaaga agaagatatg ttgtacctgc cgcctcccgc
     5281 atgaggaagc ttgtgccggc cgcgaggtgc acttcttgtc gtcgtcggtg gtggagccgt
     5341 cgccgccgcc aactccagat tcaggcaggt gcgccacaaa gctagagctg cctagccagg
     5401 tatcgacctc cgacaggtcg acgcggcccc caacaacgtc aggaggttgg ctgctgaacg
     5461 aattgccgcc ggagtttgga tccacaaccg ccattaccac cgccgcctag ggatacagag
     5521 gagagagctc ggtggagaga gatgtggttc ggccggtcgg tcggccgttt tgaccagccc
     5581 tgcctggttc cgaccgagct gggccactaa cgggcgcgag cgggcgccat ttgtcgttag
     5641 ggcagccgtc ggcctgccat gtgggccagc cctgtcaggt aagcggttaa actggctaaa
     5701 acacgatcaa cacgacttgg tagttttttg tcacaaaata taaaattttc ggtagttatc
     5761 agtctttttt tgaaaatgat agttctatgg gacggtaacc cctaatatgg tagttttttg
     5821 tcaaatactt cacggaccag tcggtgtttt gcattggtag atttgcttgg atccaatctt
     5881 gaaagaacta gcgtgacgag caaaaagacg acaatgcgtc accgatttgt gagatgaagt
     5941 tttttttttg acaactgttt gtgagatgaa gcttattaaa ctgaaaaaaa atacagtctg
     6001 cccccagcac gcgtctttca aaattcaggc tgactcggtc atcaggccgg ggtttgctta
     6061 ataattgaca cttgggcgac tggtcttctg tacagctagc aagcatatgc gcggtcactc
     6121 cagacgacca ccagatctgg atctctttat tcccctttgg gctttggtca acgcttttgc
     6181 ttggtgagca ccatcgatcg gctgcaggct gctcatggtc tactagcagc ttggcgagtc
     6241 tccgtgtccc cttccctcct ttccccaggt gagtactaat taggatgctg aatgctatag
     6301 ctgcttccct cttgtctcga tttcggaact ccagctatct gagcacctgt gtatctgctc
     6361 ctcgatttgc tttctgcagc aagtcaaggg aggaagaagg gaaggaagga gaagctgcta
     6421 gcgcctagcg gccgaagcct tatcgcttgc tgacacggaa atggacggat acgagttcga
     6481 gagggcggag atagatgcac tggagcgcgt cgtacgggat ccaagagaaa agccaataag
     6541 cctgtcgttt tggcttctca ggcgcataac gaatgatttc tctaacgcat ctgaaattgg
     6601 tcgaggtgga ttttcgaacg tttacctggt atgtacgatt aaactcatat tcccgctagg
     6661 tgcatgtcaa gcaataccta tcttgttaac actagccaca atgagtagta acatagagta
     6721 gtaatattgc ccacgttact attctatgtt actacctcta tagtgagaag tgtggtaaca
     6781 tgcaacactt tatttattag actatagact catcttgcct tgatatatgt gatgttactc
     6841 atattagtag taactagcta tgttaccaca tgcctctctt tctttattta ttacttgtca
     6901 tatcatctat tttatctaaa tatatattat gttactactt atgttacttt cattgtggat
     6961 agtctaaaaa taataacttc taactgtgat gatcaaatac atagggggtg cttccaagtg
     7021 ggttgcgtat tgctgtcaaa aagctttacc aacttactgc attggctaat gaatttgaag
     7081 atgaagttct taccacaatg agggttgctc acaaaaacat agtgcgactc ataggctact
     7141 gtcgtcacac gcaatctgaa gtcttcgaat taaaaggaga atctattttc gcacaggtca
     7201 gagaaaggtt gatctgtaca gagtatgtgc ctaatggaac tcttgatgga catatcactg
     7261 gtatcgacaa catgccatac tcaccctact actccctccg ttcggaaaaa ccttttcctt
     7321 aaatggatgt atctagcatc aagttagtgc tagatacatc catttaagaa aaaaatttgg
     7381 aataaatttt tttggacgga aggagtatta atttcccctc tttatttctt gtttcctttc
     7441 gtgtatatac atggctgttt tctatgcttc aaacatgagt tcatggatcc acatataaaa
     7501 ggggctgtcc gtcacaggtt aaagtcactc gaattccgtc catccctcta accccacttc
     7561 tctagcatca gcgacccacc cacgcatctg cttccattga cctccgtctc gcctccgctg
     7621 gtgacaccat gaccagccac actgtcccca tcaaatacaa gatctcatgg aattgtgatg
     7681 tgtacatgac ccagcggttg aataggaatt tctggatgat acatagtttc atagtatctt
     7741 ataatgattt aactggggtt gtctttgttt tgtactctgc ttaataatta ataagaatgg
     7801 ttgtgtgcat cacaaaaatg ctgcagccgg gggtttgaac cttattttcc aagagaagac
     7861 ataattttgt gttcctctat ctgtaaagca tttcagaatg acaagatcag ctccatgtca
     7921 tgtttgtaca atacattttc aattttctgc agataagttt catggaggac ttgattggga
     7981 ccagcgttat caagttctca aaggaatttg tcacggtttg cattatctgc acgatgaaat
     8041 acgcgttatc cacagagata tcaaaacaga aaatatattg ctagatgata acggcgtgcc
     8101 aaaagttttt gactttggtt tttcgaagtg ccttcctgat ggagaatctt taattaaaga
     8161 tgaagcaacc cagggaaccc tgtaagctaa cgctctttgt aattttcttt tcttttgttt
     8221 tgacagtagg tattgcgaca tccagataca gtagaatttt gtctgccaaa tgccttagat
     8281 ctaacatacg ttttgtcatg cagagggtat ttggcgccgg agtcagttga taagcaagaa
     8341 tattccttta agtccgacat atacagcttt ggtgttgtga tcatacaatt attgactgga
     8401 tcgtggatca gtaagaaccg aaatgttgag ctagtaagaa ctttattgat cacatctgaa
     8461 tgtatctagt gctagctttc tttctcgtga gttacaaaat cgatgtgaga aagtggtgct
     8521 gatttcagga acctaacatt gtgactgtct caacaattcc ataactttct aacagaaaaa
     8581 cataggatcc agatagacac aatccattct acggtttctc gattctagtg ggcctaagtg
     8641 acagccctgg ccatccacaa cgcgcaactt acttattttc ctggcttgtt ctaaaacccc
     8701 cttattttcc tggcttgagc cactgaaaca tgacactatt tttgattcta caaatttgtt
     8761 tattttgtac agaacaagaa caaatatgat catactttag cacagaaata ttgacgaacc
     8821 ttttaaaaaa gtgctcaact tttagagtga gaaaggagga gagaaggata tcgaaatgga
     8881 tagagaaatc ggatttgggt gtacactgga tggagattgt ggactaaaag aaatgtgtac
     8941 aacctccaga taactgcagt tatgtgtcta cattgaatta acttcttgca gtaagtactc
     9001 atttcccttc tggctcttgt atatggtgta aatctgtaca ggaacttaag gagttgagaa
     9061 aaaggttggt aagagaagga gcatttccat catgggaaaa caaataccac caagttagaa
     9121 catgtctgga gattgtgtat aactgcatgg acatcgaccc agataaaagg ccgactgcat
     9181 tggaaattat ccaacggctt gatgaaaccg aaagcgcgaa ctattctgtt agcagtggcc
     9241 atgcaacacc actttggcag gtacgacaaa ttgaaagaag cataaaattt cttgagaatc
     9301 agtttctaca aggccaagac ctagcacaca caatttttaa gagaatgctg acatcacact
     9361 gtcactgtat agatgctttc aaattgattt gtatatacac acgcagtcag gagatgaaga
     9421 atccgactca gcggaagtgg atgcttttga gccagagaga acaccatcaa aagacattct
     9481 ttcagatgat gaggaacccg catcagcaga cgcaaatgga gaaacgagca cacaaaaaca
     9541 taatatgcct gatcttgtaa gtaaacagtc agtgtcggcg gaactgtcct cgctagattt
     9601 ccttgagaaa atcacagatg gtttttcata cgagcaaata ctcgggaagg aaagtctgga
     9661 cgcagatgtt cataaagcat ttctttatga ggtacgcaga gttcaatatt tgcttcgatg
     9721 cgctactgat gcatcccttg gtttctactt attcttcgat cttaattatg tagacacata
     9781 cgctaaatat atgtatatat gtaaacaggg cagcatttca ggtccagcaa aggtagttgt
     9841 gaagaggtta attggagtag caattccagt tgcgaagttt aagagggatg cagaacagct
     9901 gatgagcctg gatcacaaga atatagtaaa gctccttggc tactgccacg acgaatctag
     9961 agggcataag ctggtacagt ttaaagcaaa accaccgcaa gattttaaag gagccgagca
    10021 acttctctgc tatgaatata tgcacaatgg aagcctccgt gagtatctta ctggtaatga
    10081 taccgactcc gatcacaaat cagttgctga ataactgccc ttccatggaa gggagcatat
    10141 ggcaacttcc aatacaaatc atacatgata ctattcgatc catttgtagc ttgctaagct
    10201 agctccttga tacttctgcc tgtctacttg ttgtttcata ggacaaggat ctcgtgaagt
    10261 tgattggcac atgtgctaca aattgatcaa agggacctgc gaaggtttac gctacctcca
    10321 tgagggttgt ggagatcgtc caattcttca tttgaatcta aacccgtcaa atatactgct
    10381 ggacgaaaac aacataccac gcatcacggg ttttgatttt tcgaagctca ttggtgaaaa
    10441 gaacacaaaa tccgtggttc ttaagaagaa tggaccactg tacgtaggct ctcactgcta
    10501 ccagtttata tttagccctc agttcatcat gcaatgttta tgctttgcag gcttgggact
    10561 ggtcgatttt agctagctcg ttaccgaaaa aggctttcgc cccgctttat atataaagca
    10621 acgatcaaca gcactaggta caaacgcaca cccccacaac acacgcacac acgcacccaa
    10681 ggcggaatac ataggcgttg agcgcagcaa caccaaccca aacactacac gaagaggtga
    10741 agccgcatat gacaaaccgt gggctccaag gcggcgcctt caggaagggg acgacaccgg
    10801 agcgccgcca ctgcctgatc cgaggatcag agttttccct ggagcaacac gacgagcaat
    10861 gagagccgtg gtgacgcctt caagaaggga atgagtttcg ccgccgctgg tccgtccgaa
    10921 gataaagcag gttttcaccc cggccaccac tcaccgtcac cgaacgccac accctagcta
    10981 ccacgccgcc cacacaagcc gtggcaaccg ggcagcacca agccacgggc tctgcccatg
    11041 agcaccacgg aaccatcacc agggccgccg ccccggcatc ccagaccttg acaccacctc
    11101 acccgagatc cgccgctacc ccaaccaaag aaccacgggc ggccccagtg ccaagaccca
    11161 ataggccggc caaaactagc ctccatcgac ccgttttagc tagctcgttt aaaggctgtt
    11221 acgttttaat tgaagttttg aactgatatg tttggatgca tctcctttct gtattttttt
    11281 cagaggttac ctgccaccgg acttcctgta ctcaaagggt actgacctca agtatctacc
    11341 tacggtggac atatacagct tgggtcttat aattttagag attgcaacac gtcaagagat
    11401 caagggagac catggaataa ttattaggac tgtaagagaa ttttctcaaa cctttttttt
    11461 ttttttgcgg ttgaaatttg ctcagaccta tgatcatgca atacttatgg tttggttaac
    11521 gacaaataac cgaaaaaatt catccttcat ctatatgtgt acataaatag caaaatgttt
    11581 tgtactctgt gagtatgtat tctcactcag atatcacaca tgttttgtac acttgtacac
    11641 cctgtacact tgtaaactaa tataagattt tttagatcac tacttagcac caacggatct
    11701 tatattagtt tacagaggga gtacttcttt accactctga ccatacttcg ctaatactcc
    11761 ctccgtctca taatagtgtc aaaaaacgtc ttacattatg gacggaggga gtagtttgta
    11821 gatactctga aatgagttca atgaaaattt tcaatgatag tctatataaa acatagtttg
    11881 tgtatatgct gcatatatgt acatacaaat attatattgc aaaaaataaa ggtctacatg
    11941 atattccttt tataatgttc acactgggct atcataggat aagtaattaa gtatattgaa
    12001 aataataaag agagataata tatccgctta agtggtatat tagaatgtgg atcagcacat
    12061 actcttagca cttgatggta tataatactc cctctgtaat ggagtagtgt caaaaaaaaa
    12121 acatcttgta ttaagttatg gagggagtag gtggcattat tttgtattta aatatttgct
    12181 ctgatatatt atgtaggtcg agacaaactg gaggcaagat acacaaatag caacactgta
    12241 tggttcacta gaagataaat tacgggagca agtaaaaatg tgcattgaca ttggcctaga
    12301 ctgtgtcaag tcaaacccta aaaagagacc tacagctggg gccatcatgc tctggcttga
    12361 caaagggagc aaaccgggag ttgccagacc tccactccgc gataatgtca cccatgcaga
    12421 tgatcacatc caaggtattc tgatcccaca attcttaagt ttcgattcct tcttttacat
    12481 gtaattagta ctatgctcaa acgaaaacaa aattatgaaa aatagaagtg tagtgccttc
    12541 atctactttt aagtcgattg gtaatacagg cacgatgatt attacacacg ccatttagtc
    12601 tttgtttttc tcaaaggtga accacagttc cgatctggcg aagctaatag atagtatatg
    12661 ttgatgatct cacgggtttt gtaacattta tatgttgtca gaaaaggaga agcagacgcc
    12721 attgaggaga tttttcagaa gaaagtaagc tcaagttact aaaggcatgc atgaatagac
    12781 agagtctctc tcgttcattt gcgagaaagc tgcgatggcg aaagtttctt ccagatgatc
    12841 acttgcagct ggcgtcagtg gtgccattca ggctgctgga cacatcgggt gctctgtttt
    12901 ccgctcttag ttgtcagtgc tctgtttttc ctctgtttta gagttgtaac tttcaagggg
    12961 ctggagaaac attcatttgt ccactgaact ggtgcaacct tattatgaag aggtctgctt
    13021 cggtacacac acacacacac acacccccgc gcttaacgta taagggcatt tttggcatac
    13081 ggtgcttaac gtatcccgcg ccgtatgcgg cgtacccata caggtagagc cgagcgcgcg
    13141 gctcgagccg atacggctgg ctcgcaacta ccctctcgcc gtcgcgcgaa aaaaaaaccc
    13201 acgacctgcg ctgatcacgc ggcccattcc aacgtcgccc cgccccgctg atcgccgccc
    13261 ttacccgccg atagccgcct ttccttacgc taccaattcc aacgtcctcc cgttcggccg
    13321 atcgccgcct tctccccgtc ttcacccggg atctggccgg cgcggacaga tcttcgagtc
    13381 gcccgccgta cgtgatgtcg cctccgtgca ggtcgtcgcg gtgacgtggc ctacgtggac
    13441 gccgtcgccg cccgcaactc cgtgatcaac ctctccggca acgtcctcgc cgtccgcagc
    13501 cacgccaggc gggccgaagt gcacgtgcat gccatgtaag ccttctagtt cgtcaaaaaa
    13561 attgaatctg taaataatta tgtgcaatct cttgttagat aagaatttct catctgtatg
    13621 atcgtagtac atttaatttg atttaaaatt gatagatcaa tttttattat aatattgcag
    13681 tgtaggttga gagatcattt aatggataaa gaaactggat ttacggatct gtttttggac
    13741 acgagtgagt gggatggtag cgatagtgtt gatcgtacga atggtaatga tagtaaagat
    13801 gatgatgatg gcagcgacaa tgaatcctgg tcgtcgtacg ataatttacc tgaggaggat
    13861 tttgaaaagg aggagggtgc ttgtagtgaa aacgtaagtg tcttgtacat tctttttgtc
    13921 ggatgaagaa ccatatggta cttacacgtg catatgattt ttttatgtgc aggaggatat
    13981 tgacgttcac aacgaggaaa atcatggtgt tgaatctttc ggggataaca taagccaggt
    14041 tcggtccaaa atttaagatg tcattgaata ataaatagtt ggtaatgaca acttacactt
    14101 gcttatgtgg aatattgtag gctaactttg atggttggag tggtgaagat ggggatgagc
    14161 atgaggatgg agctaatatg gacattgagg aagctgaaat ccagcaacgt gcgctggaga
    14221 cacagttgaa ggttatgggt atgacatttg catcacaatg ggaggctcac atgttctata
    14281 ataactacgc caaagaccgt ggattcagta tcagaaaaga taaggtgaaa cgaggaaaag
    14341 gactttcaac cactgttcgg tataggagat acgtttgctc gagggcagga aagcgtcgca
    14401 gtaaattttt aaacccggag ggccgtaccc gcaggctaag accagagact cgttgcgagt
    14461 gtggcgcaca tttagtcgtg aagttggaca aaggacgtgg agtttggttc gtggcagctt
    14521 ttatggatga tcacaaccat ttgttggcta gaccagatga ggtggtattt ctgcggtcac
    14581 atcgagtgat gggagatcac cagatagctg agatcttggc gatggaagga gctgggatca
    14641 gaaagcatat catcgttgac aacttcatta gcaggtatgg ctcgtatgat aagtgtgggt
    14701 ttctgagacg agacgtgtac aacctatgtt gccgagaaaa aatgaagttg attgcgaagg
    14761 gtgatgctaa cacgacaatt gggattatga ggagcagaaa agagaaggat ccagagtttt
    14821 tctttgagta cgtgctcgat aaggagggtc gtttgaagag catgttctgg tgcgacgcac
    14881 agtcgcggcg ggactatcaa ctgtatggag atgtcacggt gttgtttaaa cgacgaggga
    14941 cacgaagtga tccgtttcct tgacaggata tattggcggg taaactaagt cgctgtatgt
    15001 gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    15061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    15121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    15181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    15241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    15301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    15361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    15421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    15481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    15541 gctgaagcca gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac
    15601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    15661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    15721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    15781 aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa
    15841 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
    15901 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
    15961 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
    16021 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
    16081 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
    16141 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
    16201 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
    16261 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
    16321 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg
    16381 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
    16441 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
    16501 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc
    16561 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga
    16621 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
    16681 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
    16741 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
    16801 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatccccaa
    16861 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
    16921 tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggct
//
