LOCUS       sequence-337687-        8792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG
            pull down .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Muller M, Fah T, Schaefer M, Hermes V, Luitz J, Stalder P, Arora R, 
            Ngondo RP, Ciaudo C
  TITLE     AGO1 regulates pericentromeric regions in mouse embryonic stem cells
  JOURNAL   Life Sci Alliance. 2022 Mar 2;5(6). pii: 5/6/e202101277. doi: 
            10.26508/lsa.202101277. Print 2022 Jun.
  PUBMED    35236760
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-03-2"; volume: "5(6). pii: 5/6/e202101277. 
            doi"; pages: " 10.26508/lsa.202101277. Print 2022 Jun"
COMMENT     Sequence Label: pMSCV_Hyg_FLAG-BirA-eGFP-NLS2
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1502..1852,1853..1855,1856..2464)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             join(2498..2500,2501..2503,2504..3214)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2804..2823)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3151..3172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3215..3235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3236..3256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        3287..3786
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3322..3345)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3719..3738
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3804..4829
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3804..4829
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5187..5701
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5863..5879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5887..5893,5894..5911,5912..5917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5932..5953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6070..6087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6241..6829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6321..6340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7000..7791,7792..7860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7623..7642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7861..7965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8033..8051
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8089..8111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8211..8230
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8424..8446
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8574..8593
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctccc accatggact acaaagacga
     1441 tgacgataaa gccgccagtt taaacggtgc gccattaatt aaggatccag ctagcgccac
     1501 catgaaggac aacaccgtgc ccctgaagct gatcgccctg ctggccaacg gcgagttcca
     1561 cagcggcgag cagctgggcg agaccctggg catgagccgg gccgccatca acaagcacat
     1621 ccagaccctg cgggactggg gcgtggacgt gttcaccgtg cccggcaagg gctacagcct
     1681 gcccgagccc atccagctgc tgaacgccaa gcagatcctg ggccagctgg acggcggcag
     1741 cgtggccgtg ctgcccgtga tcgacagcac caaccagtac ctgctggacc ggatcggcga
     1801 gctgaagagc ggcgacgcct gcatcgccga gtaccagcag gccggccggg gcggccgggg
     1861 ccggaagtgg ttcagcccct tcggcgccaa cctgtacctg agcatgttct ggcggctgga
     1921 gcagggcccc gccgccgcca tcggcctgag cctggtgatc ggcatcgtga tggccgaggt
     1981 gctgcggaag ctgggcgccg acaaggtgcg ggtgaagtgg cccaacgacc tgtacctgca
     2041 ggaccggaag ctggccggca tcctggtgga gctgaccggc aagaccggcg acgccgccca
     2101 gatcgtgatc ggcgccggca tcaacatggc catgcggcgg gtggaggaga gcgtggtgaa
     2161 ccagggctgg atcaccctgc aggaggccgg catcaacctg gaccggaaca ccctggccgc
     2221 catgctgatc cgggagctgc gggccgccct ggagctgttc gagcaggagg gcctggcccc
     2281 ctacctgagc cggtgggaga agctggacaa cttcatcaac cggcccgtga agctgatcat
     2341 cggcgacaag gagatcttcg gcatcagccg gggcatcgac aagcagggcg ccctgctgct
     2401 ggagcaggac ggcatcatca agccctggat gggcggcgag atcagcctgc ggagcgccga
     2461 gaagggggga tctggccccg gcggcatggc cacaaccatg gtgagcaagg gcgaggagct
     2521 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2581 cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2641 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2701 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2761 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2821 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2881 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2941 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat
     3001 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3061 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3181 cgggatcact ctcggcatgg acgagctgta caagcctaag aagaagagga aggtgcctaa
     3241 gaagaagagg aaggtgtaag gatcctcgag gttaacgaat tctaccgggt aggggaggcg
     3301 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     3361 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     3421 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     3481 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     3541 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     3601 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     3661 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     3721 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     3781 ctttcgacct gcatcccgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3841 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3901 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3961 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     4021 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     4081 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     4141 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     4201 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     4261 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     4321 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     4381 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     4441 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     4501 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     4561 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     4621 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4681 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4741 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4801 ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag agctgatttc
     4861 gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag agaacggcct
     4921 tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct gggtcgcggg
     4981 agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg gcacgattgt
     5041 catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc cgcccgtgcc
     5101 tgccgattgg gtgcagatcc gtcgacctgc agccaagctt atcgataaaa taaaagattt
     5161 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     5221 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     5281 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     5341 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     5401 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     5461 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     5521 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     5581 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     5641 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     5701 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
     5761 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
     5821 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
     5881 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5941 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6001 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6061 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6661 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7981 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8041 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     8101 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     8161 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     8221 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     8281 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     8341 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     8401 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
     8461 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     8521 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     8581 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     8641 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     8701 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
     8761 aagattttat ttagtctcca gaaaaagggg gg
//
