LOCUS       sequence-337688-       10902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  deletion of a genomic locus in Yap1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Kagiwada S, Aramaki S, Wu G, Shin B, Kutejova E, Obridge D, Adachi 
            K, Wrana JL, Hubner K, Scholer HR
  TITLE     YAP establishes epiblast responsiveness to inductive signals for 
            germ cell fate.
  JOURNAL   Development. 2021 Oct 15;148(20). pii: 272520. doi: 
            10.1242/dev.199732. Epub 2021 Oct 19.
  PUBMED    34528691
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2021-10-15"; volume: "148(20). pii: 272520. 
            doi"; pages: " 10.1242/dev.199732. Epub 2021 Oct 19"
COMMENT     Sequence Label: pX330A-Yap1-E2-#1
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          155..382
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             403..468
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             475..495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             520..4620
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             520..4620
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4621..4668
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4696..4713)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4702..4909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4918..5058
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4918..5047
                     /label=AAV2 ITR
     promoter        5203..5414
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5427..5695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5724..5733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5754..6416
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6486..6539
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6540..7139
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6540..6559)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7036..7056
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7193..7213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7255..7376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7292..7311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7346..7365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7527..7982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7614..7633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7824..7845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7999..8018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8118..8140
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8178..8196)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8264..8368
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8369..8437,8438..9229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8587..8606)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9400..9988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9889..9908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10050..10298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10050..10070
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10221..10240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10320..10395
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        10492..10777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(10779..10902,1..154)
                     /label=chicken beta-actin promoter
ORIGIN
        1 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
       61 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      121 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      181 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      241 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc tgagcaagag
      301 gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc tggagcacct
      361 gcctgaaatc actttttttc aggttggacc ggtgccacca tggactataa ggaccacgac
      421 ggagactaca aggatcatga tattgattac aaagacgatg acgataagat ggccccaaag
      481 aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc
      541 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
      601 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
      661 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
      721 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
      781 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
      841 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
      901 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
      961 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     1021 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     1081 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     1141 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     1201 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     1261 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     1321 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     1381 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     1441 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     1501 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     1561 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     1621 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     1681 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     1741 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     1801 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     1861 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     1921 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     1981 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     2041 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     2101 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     2161 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     2221 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     2281 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     2341 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     2401 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     2461 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     2521 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     2581 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     2641 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     2701 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     2761 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     2821 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     2881 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     2941 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     3001 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     3061 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     3121 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     3181 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     3241 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     3301 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     3361 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     3421 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     3481 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     3541 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     3601 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     3661 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     3721 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     3781 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     3841 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     3901 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     3961 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     4021 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     4081 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     4141 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     4201 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     4261 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     4321 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     4381 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     4441 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     4501 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     4561 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     4621 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagta agaattccta
     4681 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4741 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4801 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4861 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg
     4921 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4981 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     5041 cgcgcagctg cctgcaggaa agagcccaca acccctcact tggtgggcca gtcctctgat
     5101 agactgtgtc ccctggatac ccgtattcta gagctagcga attcgaattt aaatcggatc
     5161 cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     5221 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     5281 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     5341 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     5401 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     5461 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     5521 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     5581 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     5641 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     5701 tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca
     5761 tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg
     5821 gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg
     5881 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg
     5941 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca
     6001 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc
     6061 gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg
     6121 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc
     6181 ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg
     6241 gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca
     6301 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca
     6361 ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat
     6421 cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat
     6481 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     6541 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6601 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6661 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6721 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6781 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6841 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6901 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6961 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7021 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7081 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     7141 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7201 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg
     7261 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7321 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7381 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc
     7441 cgggcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7501 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     7561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     7621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7681 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7741 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     7861 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     7921 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     7981 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     8041 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     8101 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     8161 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     8221 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8281 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8341 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8401 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8461 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8521 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8581 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8641 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8701 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8761 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8821 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8881 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8941 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9001 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9061 taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg ggccagatgg
     9121 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9181 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9241 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9301 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9781 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9841 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9901 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9961 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10021 ggccttttgc tggccttttg ctcacatgtg agggcctatt tcccatgatt ccttcatatt
    10081 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
    10141 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
    10201 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
    10261 tcttggcttt atatatcttg tggaaaggac gaaacaccga gggggccggc gtcttgtgcg
    10321 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
    10381 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
    10441 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac ccgttacata
    10501 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    10561 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    10621 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    10681 cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg
    10741 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
    10801 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
    10861 attattttgt gcagcgatgg gggcgggggg gggggggggg gg
//
