LOCUS       sequence-337694-        7254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing TGFB1 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Wang SK, Xue Y, Cepko CL
  TITLE     Microglia modulation by TGF-beta1 protects cones in mouse models of 
            retinal degeneration.
  JOURNAL   J Clin Invest. 2020 Aug 3;130(8):4360-4369. doi: 10.1172/JCI136160.
  PUBMED    32352930
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2020-08-3- 3"; volume: "130"; issue: "8"; 
            pages: "4360-4369"
COMMENT     Sequence Label: AAV-hRedOpsin-TGFB1
FEATURES             Location/Qualifiers
     source          1..7254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    111..132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(147..152,153..170,171..177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     190..212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     209..225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     209..225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   263..392
                     /label=AAV2 ITR
     primer_bind     complement(3769..3789)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4187..4198)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4265..4282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4271..4478
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4566..4695
                     /label=AAV2 ITR
     primer_bind     complement(4714..4731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4714..4730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4723..4745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4872..5327
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4959..4978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5169..5190
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5353..5457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5458..5526,5527..6318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5676..5695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6489..7077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6978..6997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
       61 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      181 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaga
      241 tttaattaag gccttaatta ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa
      301 gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga
      361 gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta
      421 cttatctacg tagccatgct ctaggaagat cggaattcgc ccttaagcta gcctacagca
      481 gccagggtga gattatgagg ctgagctgag aatatcaaga ctgtaccgag tagggggcct
      541 tggcaagtgt ggagagcccg gcagctgggg cagagggcgg agtacggtgt gcgtttacgg
      601 acctcttcaa acgaggtagg aaggtcagaa gtcaaaaagg gaacaaatga tgtttaacca
      661 cacaaaaatg aaaatccaat ggttggatat ccattccaaa tacacaaagg caacggataa
      721 gtgatccggg ccaggcacag aaggccatgc acccgtagga ttgcactcag agctcccaaa
      781 tgcataggaa tagaagggtg ggtgcaggag gctgaggggt ggggaaaggg catgggtgtt
      841 tcatgaggac agagcttccg tttcatgcaa tgaaaagagt ttggagacgg atggtggtga
      901 ctggactata cacttacaca cggtagcgat ggtacacttt gtattatgta tattttacca
      961 cgatcttttt aaagtgtcaa aggcaaatgg ccaaatggtt ccttgtccta tagctgtagc
     1021 agccatcggc tgttagtgac aaagcccctg agtcaagatg acagcagccc ccataactcc
     1081 taatcggctc tcccgcgtgg agtcatttag gagtagtcgc attagagaca agtccaacat
     1141 ctaatcttcc accctggcca gggccccagc tggcagcgag ggtgggagac tccgggcaga
     1201 gcagagggcg ctgacattgg ggcccggcct ggcttgggtc cctctggcct ttccccaggg
     1261 gccctctttc cttggggctt tcttgggccg ccactgctcc cgctcctctc cccccatccc
     1321 accccctcac cccctcgttc ttcatatcct tctctagtgc tccctccact ttcatccacc
     1381 cttctgcaag agtgtgggac cacaaatgag ttttcacctg gcctggggac acacgtgccc
     1441 ccacaggtgc tgagtgactt tctaggacag taatctgctt taggctaaaa tgggacttga
     1501 tcttctgtta gccctaatca tcaattagca gagccggtga aggtgcagaa cctaccgcct
     1561 ttccaggcct cctcccacct ctgccacctc cactctcctt cctgggatgt gggggctggc
     1621 acacgtgtgg cccagggcat tggtgggatt gcactgagct gggtcattag cgtaatcctg
     1681 gacaagggca gacagggcga gcggagggcc agctccgggg ctcaggcaag gctgggggct
     1741 tcccccagac accccactcc tcctctgctg gacccccact tcatagggca cttcgtgttc
     1801 tcaaagggct tccaaatagc atggtggcct tggatgccca gggaagcctc agagttgctt
     1861 atctccctct agacagaagg ggaatctcgg tcaagaggga gaggtcgccc tgttcaaggc
     1921 cacccagcca gctcatggcg gtaatgggac aaggctggcc agccatccca ccctcagaag
     1981 ggacccggtg gggcaggtga tctcagagga ggctcacttc tgggtctcac attcttggat
     2041 ccggttccag gcctcggccc taaatagtct ccctgggctt tcaagagaac cacatgagaa
     2101 aggaggattc gggctctgag cagtttcacc acccaccccc cagtctgcaa atcctgaccc
     2161 gtgggtccac ctgccccaaa ggcggacgca ggacagtaga agggaacaga gaacacataa
     2221 acacagagag ggccacagcg gctcccacag tcaccgccac cttcctggcg gggatgggtg
     2281 gggcgtctga gtttggttcc cagcaaatcc ctctgagccg cccttgcggg ctcgcctcag
     2341 gagcagggga gcaagaggtg ggaggaggag gtctaagtcc caggcccaat taagagatca
     2401 ggtagtgtag ggtttgggag cttttaaggt gaagaggccc gggctgatcc cacaggccag
     2461 tataaagcgc cgtgaccctc aggtgatgcg ccagggccgg ctgccgtcgg ggacagggct
     2521 ttccatagcg gccgccatgc cgccctcggg gctgcggcta ctgccgcttc tgctcccact
     2581 cccgtggctt ctagtgctga cgcccgggag gccagccgcg ggactctcca cctgcaagac
     2641 catcgacatg gagctggtga aacggaagcg catcgaagcc atccgtggcc agatcctgtc
     2701 caaactaagg ctcgccagtc ccccaagcca gggggaggta ccgcccggcc cgctgcccga
     2761 ggcggtgctc gctttgtaca acagcacccg cgaccgggtg gcaggcgaga gcgccgaccc
     2821 agagccggag cccgaagcgg actactatgc taaagaggtc acccgcgtgc taatggtgga
     2881 ccgcaacaac gccatctatg agaaaaccaa agacatctca cacagtatat atatgttctt
     2941 caatacgtca gacattcggg aagcagtgcc cgaaccccca ttgctgtccc gtgcagagct
     3001 gcgcttgcag agattaaaat caagtgtgga gcaacatgtg gaactctacc agaaatatag
     3061 caacaattcc tggcgttacc ttggtaaccg gctgctgacc cccactgata cgcctgagtg
     3121 gctgtctttt gacgtcactg gagttgtacg gcagtggctg aaccaaggag acggaataca
     3181 gggctttcga ttcagcgctc actgctcttg tgacagcaaa gataacaaac tccacgtgga
     3241 aatcaacggg atcagcccca aacgtcgggg cgacctgggc accatccatg acatgaaccg
     3301 gcccttcctg ctcctcatgg ccacccccct ggaaagggcc cagcacctgc acagctcacg
     3361 gcaccggaga gccctggata ccaactattg cttcagctcc acagagaaga actgctgtgt
     3421 gcggcagctg tacattgact ttaggaagga cctgggttgg aagtggatcc acgagcccaa
     3481 gggctaccat gccaacttct gtctgggacc ctgcccctat atttggagcc tggacacaca
     3541 gtacagcaag gtccttgccc tctacaacca acacaacccg ggcgcttcgg cgtcaccgtg
     3601 ctgcgtgccg caggctttgg agccactgcc catcgtctac tacgtgggtc gcaagcccaa
     3661 ggtggagcag ttgtccaaca tgattgtgcg ctcctgcaag tgcagctgaa ccggtaatca
     3721 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3781 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3841 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3901 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3961 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4021 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4081 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4141 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4201 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgaga
     4261 tctgcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4321 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4381 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4441 ggggaggatt gggaagacaa tagcaggcat gctggggact cgagttaagg gcgaattccc
     4501 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     4561 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4621 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4681 cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga
     4741 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4801 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4861 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4921 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4981 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5041 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5101 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5161 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5221 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5281 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     5341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcg
//
