LOCUS       sequence-337715-       13717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium rhizogenes-mediated hairy root transformation of Lotus
            japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. 
            NGR234..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13717)
  AUTHORS   Staehelin Lab
  TITLE     Staehelin Lab Plasmids 2021
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pISV-DsRed1-nopP
FEATURES             Location/Qualifiers
     source          1..13717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        837..1016
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1067..1618
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      1653..1853
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     primer_bind     complement(1866..1883)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1866..1882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1875..1897)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2022..2041)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2232..2253
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    2500..2524
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(4120..5268)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(5567..6361)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  6553..7320
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      7943..8654
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8781..9152)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9185..9294
                     /note="incP origin of transfer"
     primer_bind     9517..9536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9770..9787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9975..10625)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    11090..11114
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11285..11537)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(12429..12774)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(13029..13706)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(13091..13114)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     13506..13526
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(join(13716..13717,1..343))
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
       61 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
      121 ggttggaacg tcttcttttt ccacgatgtt cctcgtgggt gggggtccat ctttgggacc
      181 actgtcggta gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
      241 gaagccatct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
      301 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tctggtcttc
      361 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
      421 ctgcaggcat gcaagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga
      481 agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
      541 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
      601 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
      661 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
      721 tgccgccaag gatctgatgg cgcaggggat caagatcatg agcggagaat taagggagtc
      781 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
      841 cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc taagcacata
      901 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
      961 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     1021 ctcatattca ctctcaatcc agatctcgac tctaggggga tctaccatga gcccagaacg
     1081 acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct gcaccatcgt
     1141 caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg aaccgcagga
     1201 gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga
     1261 cggcgaggtc gccggcatcg cctacgcggg cccctggaag gcacgcaacg cctacgactg
     1321 gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac tgggctccac
     1381 gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg tggtcgctgt
     1441 catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat atgccccccg
     1501 cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg gtttctggca
     1561 gctggacttc agcctgccgg taccgccccg tccggtcctg cccgtcaccg agatctgatg
     1621 acccctagag gatcgatccc ccgatgagct aagctagcta tatcatcaat ttatgtatta
     1681 cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata taatcagtta
     1741 ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct tggactataa
     1801 tacctgactt gttattttat caataaatat ttaaactata tttctttcaa gatgggaatt
     1861 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1981 gatcgccctt cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc
     2041 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2101 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     2161 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2221 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     2281 ttcttttgat ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc
     2341 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     2401 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa
     2461 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     2521 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     2581 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     2641 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     2701 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     2761 caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta
     2821 accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa
     2881 gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc
     2941 ccctatccat tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct
     3001 gatgcatccc cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa
     3061 ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga
     3121 tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat
     3181 caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa
     3241 aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg
     3301 aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca
     3361 acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta
     3421 tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta
     3481 ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata
     3541 gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct
     3601 gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga
     3661 cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt
     3721 ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg
     3781 cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct
     3841 cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct
     3901 cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg
     3961 tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata
     4021 ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct
     4081 ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag
     4141 gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc
     4201 gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa
     4261 ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg
     4321 ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc
     4381 aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg
     4441 gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga
     4501 cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata
     4561 ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg
     4621 ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc
     4681 gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat
     4741 gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga
     4801 gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa
     4861 ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc
     4921 ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt
     4981 tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg
     5041 cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc
     5101 gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg
     5161 gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc
     5221 gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg
     5281 cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg
     5341 tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg
     5401 gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt
     5461 gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc
     5521 ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat
     5581 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     5641 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     5701 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     5761 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     5821 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     5881 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     5941 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     6001 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     6061 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     6121 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     6181 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     6241 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     6301 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     6361 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     6421 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     6481 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     6541 accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     6601 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     6661 gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac
     6721 atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga
     6781 tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa
     6841 cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg
     6901 ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa
     6961 tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac
     7021 gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt
     7081 gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag
     7141 ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg
     7201 cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat
     7261 gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc
     7321 caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca
     7381 tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct
     7441 tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc
     7501 agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg
     7561 gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc
     7621 atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg
     7681 aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc
     7741 cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg
     7801 ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg
     7861 tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg
     7921 gcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     7981 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg
     8041 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
     8101 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
     8161 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
     8221 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
     8281 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
     8341 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
     8401 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
     8461 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
     8521 gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg
     8581 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag
     8641 cgtcggaaag gcgctcggtc ttgccttgct cgtcggtgat gtacttcacc agctccgcga
     8701 agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc
     8761 cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc
     8821 ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca
     8881 ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg
     8941 cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg
     9001 attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc
     9061 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
     9121 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     9181 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     9241 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     9301 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     9361 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     9421 gcggaaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9781 gagcgaggaa gcggaagagc gccagaaggc cgccagagag gccgagcgcg gccgtgaggc
     9841 ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc gtctgacgcg
     9901 gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg gttgcgctcc
     9961 ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct gacaacgagc
    10021 ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga
    10081 ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca
    10141 acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca
    10201 acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc
    10261 tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg
    10321 ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca
    10381 ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc
    10441 tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag
    10501 taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct
    10561 acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt
    10621 gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta tcagtgataa
    10681 agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca
    10741 acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga
    10801 ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc
    10861 actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg
    10921 gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg gccggcgcca
    10981 gatctgggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc
    11041 acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc
    11101 aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcat
    11161 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
    11221 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgat ctgaattaat
    11281 tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
    11341 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
    11401 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
    11461 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
    11521 caaatgtttg aacgatcggg gaaattcgag ctcctcgagc ccggggtcga cgaattcgtc
    11581 acatgaagtc atcttcgtaa gtctcgtaag agcagctggt tgaacactgt gcggtttcgg
    11641 catcctcaaa tttgcggaga tagagcggcg gtttgtcttt acgccgccat tcagcggcac
    11701 tggtctgcgt ccatttgtcg ggatgctgtt tggggtcaag gaccatgtcg caatcatcca
    11761 cttcaacaaa ccccatcatc gctgcacggg ctttcgcctc tgggttttcc gcgcgccagt
    11821 tcagcaacgg tcgttcgccg tccctccgaa gttggtgctc gagcagaata tcgcctgcat
    11881 tctcaacgag aggatgggcg acttgaagat ccacggtgga ggtaatctct tggtgaccag
    11941 gaaattggtc tctccaactt ttggattcga actcttcttc catgctgaac ccaccttccg
    12001 ttctcatcag tccaacacac tggtttccca actggtaact gaaatatcga gcgttctcag
    12061 aatcccgacg aatgccgtat tgctgagcaa cgtccgcagt gcgtctggct tttgcggaca
    12121 cgaagtctga atattcctgt ggattgttgg cgatgtcagt gatttctttg ccatgaaatt
    12181 tcctgatctc cctcctgaaa gtctcgagat caatcggcac caccggaggt tcgtgatcga
    12241 gggtatatct ggccgccgac gatgaaccgc cgttggacct gtctaagtgc attcgggcaa
    12301 acgtgtccgc gaactcttgg gtttctgcat ccatttgctt gctggcactc tgcgtgtaat
    12361 ggaaatcgga cgagctatca attcgaccgt acatcatcga tgttaacgga tccgcggccg
    12421 ctctaggatc tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
    12481 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
    12541 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    12601 tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga
    12661 tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg acagatagct
    12721 gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt ctcaatcgga
    12781 cctgcaggca tgcaagcttg catgcctgca ggtcactgga ttttggtttt aggaattaga
    12841 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
    12901 caacacatga gcgaaaccct ataagaaccc taattccctt atctgggaac tactcacaca
    12961 ttattctgga gaaaaataga gagagataga tttgtagaga gagactggtg atttttgcgg
    13021 actctagatc acaggaacag gtggtggcgg ccctcggtgc gctcgtactg ctccacgatg
    13081 gtgtagtcct cgttgtggga ggtgatgtcc agcttggcgt ccacgtagta gtagccgggc
    13141 agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg
    13201 tccttcagct tcagggcctt gtgggtctcg cccttcagca cgccgtcgcg ggggtacagg
    13261 cgctcggtgg aggcctccca gcccatggtc ttcttctgca tcacggggcc gtcggagggg
    13321 aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag ggaggagtcc
    13381 tgggtcacgg tcgccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
    13441 tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac gtacaccttg
    13501 gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
    13561 gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc ctcgccctcg
    13621 atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg catgaactcg
    13681 gtgatgacgt tctcggagga ggccattgtc tcgaggt
//
