LOCUS       sequence-337720-       13166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium rhizogenes-mediated hairy root transformation of Lotus
            japonicus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13166)
  AUTHORS   Staehelin Lab
  TITLE     Staehelin Lab Plasmids 2021
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pISV-DsRed1
FEATURES             Location/Qualifiers
     source          1..13166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..56
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(303..324)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     515..534
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     659..681
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     673..690
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     674..690
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(703..903)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(938..1489)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1540..1719)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2213..2557
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2567..3244
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2747..2767)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3159..3182
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3760..4105
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4185..4437
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4608..4632
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5097..5747
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(5935..5952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6186..6205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            6428..6537
                     /note="incP origin of transfer"
     CDS             6570..6941
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      7068..7779
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  8402..9169
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             9361..10155
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             10454..11602
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
       61 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggttttt
      121 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
      181 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
      241 ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg
      301 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      361 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg
      421 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      481 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      541 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      601 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      661 cagtcacgac gttgtaaaac gacggccagt gaattaattc ccatcttgaa agaaatatag
      721 tttaaatatt tattgataaa ataacaagtc aggtattata gtccaagcaa aaacataaat
      781 ttattgatgc aagtttaaat tcagaaatat ttcaataact gattatatca gctggtacat
      841 tgccgtagat gaaagactga gtgcgatatt atgtgtaata cataaattga tgatatagct
      901 agcttagctc atcgggggat cgatcctcta ggggtcatca gatctcggtg acgggcagga
      961 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt
     1021 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt
     1081 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct
     1141 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt
     1201 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc
     1261 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat
     1321 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac
     1381 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt
     1441 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagatcccc
     1501 ctagagtcga gatctggatt gagagtgaat atgagactct aattggatac cgaggggaat
     1561 ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta
     1621 ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca
     1681 gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
     1741 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     1801 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     1861 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     1921 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     1981 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     2041 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
     2101 gtgcttgcgg cagcgtgaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc
     2161 tcgtctactc caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt
     2221 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     2281 tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag
     2341 gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca
     2401 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     2461 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     2521 cctctatata aggaagttca tttcatttgg agaggacctc gagacaatgg cctcctccga
     2581 gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca
     2641 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa
     2701 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2761 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
     2821 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc
     2881 gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat
     2941 cggcgtgaac ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc
     3001 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct
     3061 gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa
     3121 gcccgtgcag ctgcccggct actactacgt ggacgccaag ctggacatca cctcccacaa
     3181 cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct
     3241 gtgatctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     3301 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     3361 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     3421 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagcttg
     3481 catgcctgca ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg
     3541 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
     3601 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
     3661 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     3721 cacgtcttca aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt
     3781 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     3841 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     3901 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3961 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     4021 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     4081 aagttcattt catttggaga ggagatccta gagcggccgc ggatccgtta acatcgattc
     4141 tagagaattc gtcgaccccg ggctcgagga gctcgaattt ccccgatcgt tcaaacattt
     4201 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     4261 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     4321 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     4381 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     4441 aattaattca gatcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
     4501 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
     4561 catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     4621 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     4681 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttccccagat
     4741 ctggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc cgacagcgcg
     4801 cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag aatgccatag
     4861 tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag caccggcata
     4921 atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat caggggtatg
     4981 ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac gcgcggattc
     5041 tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg tcaagcatga
     5101 caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg
     5161 tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag ccggcgcttt
     5221 actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg
     5281 agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt ctgatcggga
     5341 atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg
     5401 gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg
     5461 aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc tacttcactg
     5521 ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc ggcggcaccg
     5581 ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc gacgaagccg
     5641 gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg gcgaaaagga
     5701 ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca ggaccgctgc
     5761 cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc cccctttcca
     5821 ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc ctagcgtcca
     5881 agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg cttcctcgct
     5941 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6001 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgcttttccg
     6301 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     6361 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     6421 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     6481 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     6541 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     6601 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg
     6661 cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa
     6721 tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg
     6781 gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg
     6841 gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca
     6901 aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac
     6961 ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagagcgac
     7021 ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg cctttccgac
     7081 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
     7141 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct
     7201 cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga
     7261 ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag
     7321 ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat
     7381 gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga
     7441 cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca
     7501 cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg
     7561 cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa
     7621 ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc
     7681 ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc
     7741 gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc cttgccattg
     7801 ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc ggaagcattg
     7861 acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt gagggcggcg
     7921 gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac ttcatggcgg
     7981 ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc gtgctcgtgt
     8041 tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     8101 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     8161 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     8221 tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat ttcagggggc
     8281 aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc
     8341 atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt
     8401 gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt
     8461 catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct
     8521 gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca
     8581 gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt
     8641 caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata
     8701 cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg
     8761 atttagcccc gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc
     8821 ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt
     8881 gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat
     8941 atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca
     9001 tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcggtgcttt tgccgttacg
     9061 caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc
     9121 acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc ataattgtgg
     9181 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
     9241 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     9301 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     9361 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     9421 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     9481 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     9541 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     9601 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     9661 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     9721 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     9781 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     9841 gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     9901 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     9961 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
    10021 attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta
    10081 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
    10141 gatgaattgt tttagtacct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac
    10201 cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg
    10261 caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
    10321 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
    10381 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
    10441 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
    10501 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
    10561 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg
    10621 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
    10681 gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
    10741 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
    10801 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    10861 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    10921 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    10981 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    11041 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    11101 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    11161 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    11221 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    11281 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    11341 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    11401 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    11461 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    11521 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    11581 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    11641 cagcgcttta ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca
    11701 gtatcgctcg ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg
    11761 acaattgtga ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc
    11821 gagatccgat tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac
    11881 gaggagaaaa agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc
    11941 gcctacatcg acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag
    12001 gacgctcaca aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg
    12061 gtcgccggta tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga
    12121 cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg
    12181 ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg
    12241 gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg
    12301 atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg
    12361 ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt
    12421 tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc
    12481 tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt
    12541 gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg
    12601 atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca
    12661 gttgtttcct tactgggctt tctcagcccc agatctgggg tcgatcagcc ggggatgcat
    12721 caggccgaca gtcggaactt cgggtccccg acctgtacca ttcggtgagc aatggatagg
    12781 ggagttgata tcgtcaacgt tcacttctaa agaaatagcg ccactcagct tcctcagcgg
    12841 ctttatccag cgatttccta ttatgtcggc atagttctca agatcgacag cctgtcacgg
    12901 ttaagcgaga aatgaataag aaggctgata attcggatct ctgcgaggga gatgatattt
    12961 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    13021 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    13081 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    13141 tgtatcgagt ggtgattttg tgccga
//
