LOCUS       sequence-337723-       11930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 11930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.1
FEATURES             Location/Qualifiers
     source          1..11930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4502..5075
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4525..5077
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4669..4686)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4896..4915
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5092..5101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5098..5661
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     primer_bind     complement(9962..9979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10133..10721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10213..10232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10892..11683,11684..11752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11515..11534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11753..11857)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccccccccg atccccggaa cgccagccgc catccaccgc ccgcagcccc cggagcccgc
       61 ggaccccgac cccccgccgc tcgcccgccg ttgcgccgct ctctgcgggg gggctagggg
      121 gccgcggggg gaaggccacg ccccctccac tttttccagg aatgcgcggc atgacccgcc
      181 ccccccatga cccgccaaca cccccccgat gccccccggg ggtctttcct ggggggcctt
      241 tgtcagggtt gcctgtgtca cccacgggcc ccttggccac gcttgcctgg gtcaccccgg
      301 agccccgggt cacgtttgcc ggggtcagcc ctggggcgct tcttcacgct ccattgccat
      361 gcagaattca ctagtgatta gaatcccacg ttgtgcatag aaacagatgc aggcaaaaca
      421 tccacacata taaaacaaaa aattaaaacc aataaaactc ctaaactttt ggtctttctt
      481 gaatcttcaa tccctcaggt tatgaaataa tcatttatgc agtcaaaaat ttgccattct
      541 tgttgccagg tgtggtgatg attcggggaa gcagaagcag gcagatctct gtgaatgagg
      601 ccagcctggt ctacaaagtg agtcccagga cagtcaggcc tgttacacag agaaaccttg
      661 aaaaaaaaaa gataatatgt actgttgtat taccccaata tataaggcta aaccattaga
      721 agcacaacac tgttaagtac ggaaaataat atctagtgtg gtacagttac tactactata
      781 atacactaat atagctgtgg gaaactagtt ccaaagatga attactaacc agtgtttcca
      841 aggaaataaa tgaaagcaga gagattagtt ctattgctag tgtttcattt tcgtatattt
      901 cttacaattt ctcttgttac aaataggcac tagggtatca agataatttt aacgactggc
      961 tgagaaccct agaaaatctc tgtgaaaaag ggatttgtga aatgagagag ggtaatgtgg
     1021 ccattataga aaaggctttt gtgtgccttg catgcataga ccctgtgttt gatctcttaa
     1081 caccctcctt gaccagaaaa agcttctgtg gatagaaaat gattagttat atatactttt
     1141 agggaaacgt agttctggat tctttggtta caattaacag aattaagtgc aaacaaagcc
     1201 agaaacctcc tgataaatga gaaaacctgc ttgtagaagg ttgtaaggct ctgtaatata
     1261 ggaattagga gaaaagaaac ctgtgtggtg gggcacgtct gtaatcccag cattgggaag
     1321 tagaggtaga agattagaaa tcaaaggcca gcctcagcaa cacagtgagt ttgaggccac
     1381 cctgaactac atcaggttct gtctcctttc tttttttttt ttttcttttc tttttttggt
     1441 ttctctgtgt agttttggag cctatcctgg cactagctct gaagagcagg ctggcctcga
     1501 actcagagat cagccagcct ctgctgggat taaaggtatg caccaccaac gccccaggtt
     1561 ttgtctcaaa caaacaaaaa taacatcagg aggtggtgag agggctcagt ggtcacaggc
     1621 attctctgca aagcctgact ctgagttgga tcctttagag ctacatggtt gagggaagag
     1681 aactgactcc tggaaggtgt cctctggtcc ccacacatag ctatacacag catgtgcatt
     1741 cacacacact aaataatgct atttttaaaa aaattaaaaa caacaacagt ttgggttgtg
     1801 aaaactagaa ctagataata ggtaagaatc aagtatcatg taaatttgct ttcaactcat
     1861 cccaaaattt gttttatatt tcagtttttt tccttcctag cttgactgtg gagtcttgtc
     1921 cggaagcaaa tagttccttt gcagatccca catgtggaca ccggacagta ggtcctcaaa
     1981 tgctccttat taggttggtt caataatatc aattgtttgt tactaggcag tgatgttgta
     2041 catctggagg agatctcttg agcccataat caggttatta ggaataaata ctctaaggct
     2101 aaaaatgtag cttagtgata agagtgcttg cctggtgtgc tgagaccctc ggttccatct
     2161 ccacaacccc atattccatt acaaaatacc ttttcaccgt ccctagcatt aagaaacaaa
     2221 acaacaaaga agtttttctt tcttctgaga tcctgcccgg agaggcattt aaaactggcc
     2281 agggccaaaa aaaaaaaaaa aagaaaaaaa agaaaagaaa acaggctagg gccggcatgg
     2341 tggcgcacgc ctttaatccc agcacgcagg aggcagaggc agggcggatc tctgtgagtt
     2401 tgaggtcagc ctggtctacc tagtgagttt cagggcaccc agggctaaag agactgtctc
     2461 aaaaacaaaa cagccacaca atcagaacca cagcaaaacg cagttatgat ccttggaact
     2521 gtaggaatga caagcattta aataatagga cgagccattt ttgagaagct ctgatttcac
     2581 aagtgtcagg gatgggctct gggcgagtaa gattgctaat gctggcctct aaatgagacc
     2641 acgtggagtt gattagattc ttttcatgtt cctcgtgctc tatcaaataa ctgtacccaa
     2701 atacacacac acacacacac acacaatgcg cgcacacaca aaatcctttt ttagcttaag
     2761 aagcccagaa tcagaagtaa agctaactgt gggacttaag tattattctg aacggaactc
     2821 ccagggcgtg aagcgcgctt caggcttcca gagaagcagc tggcgctgga tggaatgaac
     2881 caagaggcca gcacaggggc agatccgtcg agctctcggc caccgagctg agcccttagg
     2941 ttctggggct gggaagggtc cctaggattg tgcacctctc ccgcggggga caagcagggg
     3001 gatggcgggg ctgacgtcgg gaggtggcct ccacgggaag ggacacccgg atctcgacac
     3061 agccttggca gtggagtcag gaagggtagg gacagattct ggacgccctc ttggccagct
     3121 cctcaccgcc ccacccccga ctggagccga gagtaattca tacaaaagga gggatcgcct
     3181 tcgcccctgg gaatcccagg gaccgtcgct aaattctggc cggcctccca gcccggaacc
     3241 gctgtgcccg cccagcgcgg cgggaggagc ctgcgcctag ggcggatcgc gggtcggcgg
     3301 gagagcacaa gcccacagtc cccggcggtg ggggaggggc gcgctgagcg ggggcccggg
     3361 agccagcgcg gggcaaactg ggaaagtggc gtcgtgtgct ggctccgccc tcttcccgag
     3421 ggtgggggag aacggtataa aagtgcggta gtcgcgttgg acgttctttt tcgcaacggg
     3481 tttgccgtca gaacgcaggt gagtggcggg tgtggcctcc gcgggcccgg gctccctcct
     3541 ttgagcgggg tcgggccgcc gtgcgggtgt cgtcggccgg gcttctctgc gagcgttccc
     3601 gccctggatg gcgggctgtg cgggagggcg agggggggag gcctggcggc ggccccggag
     3661 cctcgcctcg tgtcgggcgt gaggcctagc gtggcttccg ccccgccgcg tgccaccgcg
     3721 gccgcgcttt gctgtctgcc cggctgccct cgattgcctg cccgcggccc gggccaacaa
     3781 agggagggcg tggagctggc tggtagggag ccccgtagtc cgcatgtcgg gcagggagag
     3841 cggcagcagt cggggggggg accgggcccg cccgtcccgc agcacatgtc caacgccgcc
     3901 tggacgggta gcggcctgtg tcctgataag gcggccgggc ggtgggtttt agatgccggg
     3961 ttcaggtggc cccgggtccc ggcccggtct ggccagtacc ccgtagtggc ttagctccga
     4021 ggagggcgag gcccgcccgc ccggcaccag ttgcgtgcgc ggaaagatgg ccgctcccgg
     4081 gccctgtagc aaggagctca aaatggagga cgcggcagcc cggcggagcg gggcgggtga
     4141 gtcacccaca caaaggaaga gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg
     4201 tggtgtaccg gccgcacttc agtcaccccg ggcgctcttt cggagcaccg ctggcctccg
     4261 ctgggggagg ggatctgtct aatggcgttg gagtttgctc acatttggtg ggtggagact
     4321 gtagccaggc cagcctggcc atgaaagtaa ttcttggaat ttgcccattt tgagtttgga
     4381 gcgaagctga ttgacaaagc tgcttagccg ttcaaaggta ttcttcgaac ttttttttta
     4441 aggtgttgtg aaaacctcga ggtacgtatc gcgaaagcta gcggccgcgc agttaacgcc
     4501 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4561 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4621 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4681 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4741 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4801 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4861 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4921 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     4981 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5041 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaagatc tgccaccatg
     5101 gttcgaccat tgaactgcat cgtcgccgtg tcccaaaata tggggattgg caagaacgga
     5161 gacctaccct ggcctccgct caggaacgag ttcaagtact tccaaagaat gaccacaacc
     5221 tcttcagtgg aaggtaaaca gaatctggtg attatgggta ggaaaacctg gttctccatt
     5281 cctgagaaga atcgacctct aaaggacaga attaatatag ttctcagtag agaactcaaa
     5341 gaaccaccac gaggagctca ttttcttgcc aaaagtttgg atgatgcctt aagacttatt
     5401 gaacaaccgg aattggcaag taaagtagac atggtttgga tagtcggagg cagttctgtt
     5461 taccaggaag ccatgaatca accaggccac ctcagactct ttgtgacaag gatcatgcag
     5521 gaatttgaaa gtgacacgtt tttcccagaa attgatttgg ggaaatataa acttctccca
     5581 gaatacccag gcgtcctctc tgaggtccag gaggaaaaag gcatcaagta taagtttgaa
     5641 gtctacgaga agaaagacta atctagcata ttacccctaa cacctgccac cccagtctta
     5701 atcagtggtg gaagaacggt ctcagaactg tttgtctcaa ttggccattt aagtttaata
     5761 gtgaaagact ggttaatgat aacaatgcat cggaaaacct tcaggaggaa aggagaatgt
     5821 tttgtggaac atttttgtgt gtgtggcagt tttaagttat tagttttcaa aatcagtact
     5881 ttttaatgga aacaacttga ccaaaaatct gtcacagaat tttgagaccc attaaaatac
     5941 aagtttaatg agaagtctgt ctctgttaat gctgaagtca ttactaagtg cttagcttag
     6001 caaggtatgt ggatgcccat ttgtgttcca agggattgga ctgttcatca ggacccagag
     6061 ctgagtttca agggctcaag agatggctta ttacctgtgg gtgtcttgaa ggttctggtt
     6121 gggacaaatt aggaatgttt ttggcagaca tggtgactac ccttcatctg ggtgagttca
     6181 gttgatttgt cttgagcctt tggggtttac acaagtaaat gacatcatac agttagtgta
     6241 ttgttagtga atattaatat atgaggcagg ctttgctcta gcaattttag aactagtttt
     6301 caggaaaggg ttcatcttgt gcattggatg tttgattcta tcacttagag tttaaactga
     6361 aagtgctcaa gaggttttat ttaggctggg atataaataa gcctttctgt agcttgtaat
     6421 ggtatcagga atttaaaagg ccatctgggg cacaaagatt aagcagaaaa ggtagaaggt
     6481 gagattgggg gactttgagt acttcacaca ctttaatgtg tgagtgcttt agtgcatata
     6541 gtacaactgc cagataaggg catccacatc tgattgtttg gaaggcacct tgtggtttct
     6601 gggaattcag aattgggaga aaaagtgctc ccaaccgctg aagcccttgg taatctgcag
     6661 ggtgtttatt tagcaggaga taaggacaaa aagttatagt gtggagttgg ttgagttggt
     6721 agatgtcatt acaacaggtg gtcttaaatt gggttaggag tcactttgaa atacctgggc
     6781 cataagcaaa gtggcatttt cacctttcag gagaaactgg tacacttatc cattctatag
     6841 tgcatgcttg ttcaattggg ctgatgacta aaccggtgac taaaggtttg tcagtataaa
     6901 tggatgggtt gtaggcagac ggtgaggaat tactatacct gcaaggagtc attgcctgat
     6961 ctgcctggaa aggggcagga ttgagtctca gaaggtgtac accataggat atggaaaaat
     7021 ttgtcacgcc tagcattcaa cttagtggtg tagcgccacc tactggcact ttaaaagctt
     7081 agcatagagg agcatgtgtg ttaggagctc ggatgggatc cagggcctca aggtttgcat
     7141 gtaaataaaa gccctttacc aaattaacta cataccagca tacatcagtc ctttagtgtt
     7201 gaaaaacaga agtgaaagct aatatatata gtgcttgctt tatttaagtc tagctgatta
     7261 cgtgtttggt tgccagtgtg actagtctgg agttgaattt gtcctcagac acgtaaaatg
     7321 gaatttggga ttcacaacac tctagtatga gggacctaat ggcctgtacc aggcacaaac
     7381 gtgtctataa actacacaaa acgaaggaat ttacaggaat taggaaggta ttcttaacat
     7441 taaaacatta tgggcatttt aaaaaaagct ttgacaggat ttctttgtca tggctgtcct
     7501 ggagctagtt gtgtagacca ggctgggctg aaatcttgtc tgcctgcctg gcttggacac
     7561 ttttttatta tgtatacaac attctgcttc catgtatatc tgcacattag aagacggcac
     7621 cagatctcct aatggatggt tgtgagccac catgtggttg ctgggaattg aactcaggac
     7681 ctctggaaga gcagtgctct taacctctga gccatctcca gccccagctt gggcacattt
     7741 ttaatggctg ggaaatcaaa ccccctaggc cttctgtcag taatgaaggg cttttggcta
     7801 ccgagagtag gatttaaggt tattcggagc tgcaggtctg cctcagtgca ggtttgggag
     7861 tccagcatct tagaaaatgc agtgaagcca agctgagcta tattttgttt aaaaaaaaaa
     7921 taagtgggta aagtgctgct gagcctgatg accaagctgg gacacaagta gaagaacata
     7981 ggccaatgct ctatattaaa agcatgggtc atttttaatg ctcttgagaa ggctatgcct
     8041 acactactct cagccaccgc agcgtgttta aattaaacta gtttggaaat tttctttggg
     8101 ggtaagctat ttaacctagt gccttggcag gtatactact gaactctcct cctcattcct
     8161 ttttgttttt taagaatttc agtcaggctc aggcagccct taaacttgtg attaagcctg
     8221 agaacagtta cgattatgag cctattagta taccgatcaa tatgtgaatt tttttgggat
     8281 gggggtcagg cctccctgcc tcccaaatac tgggactaaa ggctgcacca ccacaacctg
     8341 gctcttgaaa tacttttcta cattttttgg ggggcatggg tgggagagca gggtttctct
     8401 gtattagccc tggctctcct ggaactctgt agaccaggct attcttgagc tcagattagc
     8461 ctgtctctgc ctcctaaatt ctgggattaa aggtgtgtgc tactgctgcc tggctacaaa
     8521 gacatttttt tttttcttaa atttaaaaac aaaagtggtt cttttagaag ggtggttggt
     8581 gttggcacat actccaagca ctcaggtttt gagtttgtcc caggaatgaa gactgcatta
     8641 ctgccgcccc tccctggtaa gggctacaca gagaaatcct atttggagcc tgtcctggta
     8701 actcgctctg tagaccaggc tggcctcgaa ctcaagagaa ccacctgcct ctgaatgctg
     8761 gtattaaggg caggcaccac caacacccag cctaaaaaat gtcttttttt taaagatttt
     8821 tttttttttt ttacagaata aacattctgt ttacaatatt ctgcttctat gtatatctgc
     8881 acactagaag agggcacccg atctcataat ggatggttgt gagccaccaa gtggttgctg
     8941 ggaattgaac tcagaacctc tggaagagca gtcagtgctc ttaacctctg agccatctct
     9001 ccagccccta aaaatggctc ttgagatagg gtctcaagta gtttgagact gagttggcta
     9061 tataaacaag gctggcacat agcaccatgt acagctgggt ttagtttaca tggggtgttt
     9121 ttgtctctgg aggcaggagg atcatttgag catagggagt taatagtgag gtcatgtttt
     9181 atctactctt ctgaattgag aactaagctg atgcaaagca agtttgactg aagaagtcca
     9241 gtttatgaga acaagggtgg aaactaatgt gtcaaagatg gccttgcatg tgttttagat
     9301 gatgacccag tcacttggga attactggat gtgtaagacc tatatcttga caggagtgaa
     9361 cagtgtctta taggtcctat atgaaagaaa tgagacatac ccattttgtt tcccctaaga
     9421 attcactttt cctaacctgg ttcatgctat ttaggttatt ttacttgcaa atcctagggt
     9481 gctcccttac ccagtattgc ttatgtggca ccaaagtcac tcactcccat gatttgcaag
     9541 tctctgggaa cttccatgac aacctagaat agcaactcaa atacattttc tcagtaccaa
     9601 ttttgaagaa aaaatatttt gcaaaatagc tgtatggatg ggtactaaat agtgaggtta
     9661 tctccagaag gcctatgaag aattaaggtt gagttcagtt gagttcagca gcaagtttaa
     9721 ggttcatcca tttttgtaca gtgttttcct attacggtaa gtgttttgcc tgcaggaata
     9781 tctgtaccac atgcttgcct ggtacctata tcggccagaa gagggctttg gatcctctgg
     9841 acttgaatta cagatgggta ttagccacca tttaggtgct gggaattgaa accaagtcct
     9901 ctggaagaac agcaagtgac tcgacgtccg gagctctggc gcgccatggc tcttccgctt
     9961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10561 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11881 ctgcatctca tcacggtcac gcatggctgc gcccgcgcag cgccccccga
//
