LOCUS       sequence-337728-        8682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses STA5B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: MAC_N_STA5B
FEATURES             Location/Qualifiers
     source          1..8682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(827..1177,1178..1180,1181..1789)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1790..1816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1847..1870
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1907..1930
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1943..1967
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4338..4362)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4400..4417)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4406..4630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4763..4782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4914..4961
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4969..5991
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4969..5991
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6121..6242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6158..6177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6212..6231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6291..6307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6291..6307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6304..6326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6315..6331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6339..6345,6346..6363,6364..6369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6384..6405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6522..6539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6693..7281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6773..6792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7452..8243,8244..8312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8075..8094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8313..8417)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8492..8511)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagaccatga aggacaacac
      841 cgtgcccctg aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct
      901 gggcgagaca ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga
      961 ctggggcgtg gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca
     1021 gctgctgaac gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc
     1081 cgtgatcgac agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga
     1141 cgcctgtatc gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag
     1201 ccccttcggc gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc
     1261 cgctattggc ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg
     1321 cgccgacaaa gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc
     1381 cggcatcctg gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc
     1441 tggcatcaac atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac
     1501 cctgcaggaa gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga
     1561 gctgagagcc gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg
     1621 ggagaagctg gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat
     1681 cttcggcatc agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat
     1741 catcaagccc tggatgggcg gcgagatcag cctgcggagc gccgagaagt acccatacga
     1801 tgttcctgac tatgccggta ccgagctcgg atccaccatg gctagctgga gccacccgca
     1861 gttcgagaaa ggtggaggtt ccggaggtgg atcgggaggt ggatcgtgga gccacccgca
     1921 gttcgaaaaa gcggccgata tcacaagttt gtacaaaaaa gcaggctcca ccatggctgt
     1981 gtggatacaa gctcagcagc tccaaggaga agcccttcat cagatgcaag cgttatatgg
     2041 ccagcatttt cccattgagg tgcggcatta tttatcccag tggattgaaa gccaagcatg
     2101 ggactcagta gatcttgata atccacagga gaacattaag gccacccagc tcctggaggg
     2161 cctggtgcag gagctgcaga agaaggcaga gcaccaggtg ggggaagatg ggtttttact
     2221 gaagatcaag ctggggcact atgccacaca gctccagaac acgtatgacc gctgccccat
     2281 ggagctggtc cgctgcatcc gccatatatt gtacaatgaa cagaggttgg tccgagaagc
     2341 caacaatggt agctctccag ctggaagcct tgctgatgcc atgtcccaga aacacctcca
     2401 gatcaaccag acgtttgagg agctgcgact ggtcacgcag gacacagaga atgagttaaa
     2461 aaagctgcag cagactcagg agtacttcat catccagtac caggagagcc tgaggatcca
     2521 agctcagttt ggcccgctgg cccagctgag cccccaggag cgtctgagcc gggagacggc
     2581 cctccagcag aagcaggtgt ctctggaggc ctggttgcag cgtgaggcac agacactgca
     2641 gcagtaccgc gtggagctgg ccgagaagca ccagaagacc ctgcagctgc tgcggaagca
     2701 gcagaccatc atcctggatg acgagctgat ccagtggaag cggcggcagc agctggccgg
     2761 gaacggcggg ccccccgagg gcagcctgga cgtgctacag tcctggtgtg agaagttggc
     2821 cgagatcatc tggcagaacc ggcagcagat ccgcagggct gagcacctct gccagcagct
     2881 gcccatcccc ggcccagtgg aggagatgct ggccgaggtc aacgccacca tcacggacat
     2941 tatctcagcc ctggtgacca gcacgttcat cattgagaag cagcctcctc aggtcctgaa
     3001 gacccagacc aagtttgcag ccactgtgcg cctgctggtg ggcgggaagc tgaacgtgca
     3061 catgaacccc ccccaggtga aggccaccat catcagtgag cagcaggcca agtctctgct
     3121 caagaacgag aacacccgca atgattacag tggcgagatc ttgaacaact gctgcgtcat
     3181 ggagtaccac caagccacag gcacccttag tgcccacttc aggaatatgt ccctgaaacg
     3241 aattaagagg tcagaccgtc gtggggcaga gtcggtgaca gaagaaaaat ttacaatcct
     3301 gtttgaatcc cagttcagtg ttggtggaaa tgagctggtt tttcaagtca agaccctgtc
     3361 cctgccagtg gtggtgatcg ttcatggcag ccaggacaac aatgcgacgg ccactgttct
     3421 ctgggacaat gcttttgcag agcctggcag ggtgccattt gccgtgcctg acaaagtgct
     3481 gtggccacag ctgtgtgagg cgctcaacat gaaattcaag gccgaagtgc agagcaaccg
     3541 gggcctgacc aaggagaacc tcgtgttcct ggcgcagaaa ctgttcaaca acagcagcag
     3601 ccacctggag gactacagtg gcctgtctgt gtcctggtcc cagttcaaca gggagaattt
     3661 accaggacgg aattacactt tctggcaatg gtttgacggt gtgatggaag tgttaaaaaa
     3721 acatctcaag cctcattgga atgatggggc cattttgggg tttgtaaaca agcaacaggc
     3781 ccatgaccta ctcattaaca agccagatgg gaccttcctc ctgagattca gtgactcaga
     3841 aattggcggc atcaccattg cttggaagtt tgattctcag gaaagaatgt tttggaatct
     3901 gatgcctttt accaccagag acttctccat tcggtcccta gccgaccgct tgggagactt
     3961 gaattacctt atctacgtgt ttcctgatcg gccaaaagat gaagtatact ccaaatacta
     4021 cacaccagtt ccctgcgagt ctgctactgc taaagctgtt gatggatacg tgaagccaca
     4081 gatcaagcaa gtggtccctg agtttgtgaa cgcatctgca gatgccgggg gcggcagcgc
     4141 cacgtacatg gaccaggccc cctccccagc tgtgtgtccc caggctcact ataacatgta
     4201 cccacagaac cctgactcag tccttgacac cgatggggac ttcgatctgg aggacacaat
     4261 ggacgtagcg cggcgtgtgg aggagctcct gggccggcca atggacagtc agtggatccc
     4321 gcacgcacaa tcgttggacc cagctttctt gtacaaagtg gtgacgtaag ctaggggccc
     4381 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4441 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4501 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4561 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4621 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4681 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4741 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4801 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4861 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc
     4921 ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa
     4981 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     5041 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     5101 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     5161 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     5221 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     5281 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     5341 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     5401 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     5461 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     5521 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     5581 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     5641 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     5701 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     5761 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     5821 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     5881 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     5941 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta
     6001 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6061 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6121 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6181 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6241 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7261 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7321 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7561 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7921 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7981 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8041 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8101 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8161 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8221 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8281 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     8461 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8521 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8581 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     8641 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
