LOCUS       sequence-337731-       12273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.2-Neo
FEATURES             Location/Qualifiers
     source          1..12273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     243..262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             397..1191
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(451..470)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1061..1080
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1365..1486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1402..1421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1456..1475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1537..1554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1708..2296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1788..1807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2467..3258,3259..3327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3090..3109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3328..3432)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(12269..12273,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
      361 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
      421 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      481 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      541 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      601 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      661 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      721 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      781 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      841 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      901 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
      961 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     1021 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1081 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1141 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     1201 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     1261 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     1321 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     1381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     1501 cgtcgacctc taggcgcgcc atggctcttc cgcttcctcg ctcactgact cgctgcgctc
     1561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3421 aggggttccg cgcacatttc cccgaaaagt gccacctgca tctcatcacg gtcacgcatg
     3481 gctgcgcccg cgcagcgccc cccgaccccc ccccgatccc cggaacgcca gccgccatcc
     3541 accgcccgca gcccccggag cccgcggacc ccgacccccc gccgctcgcc cgccgttgcg
     3601 ccgctctctg cgggggggct agggggccgc ggggggaagg ccacgccccc tccacttttt
     3661 ccaggaatgc gcggcatgac ccgccccccc catgacccgc caacaccccc ccgatgcccc
     3721 ccgggggtct ttcctggggg gcctttgtca gggttgcctg tgtcacccac gggccccttg
     3781 gccacgcttg cctgggtcac cccggagccc cgggtcacgt ttgccggggt cagccctggg
     3841 gcgcttcttc acgctccatt gccatgcaga attcactagt gattagaatc ccacgttgtg
     3901 catagaaaca gatgcaggca aaacatccac acatataaaa caaaaaatta aaaccaataa
     3961 aactcctaaa cttttggtct ttcttgaatc ttcaatccct caggttatga aataatcatt
     4021 tatgcagtca aaaatttgcc attcttgttg ccaggtgtgg tgatgattcg gggaagcaga
     4081 agcaggcaga tctctgtgaa tgaggccagc ctggtctaca aagtgagtcc caggacagtc
     4141 aggcctgtta cacagagaaa ccttgaaaaa aaaaagataa tatgtactgt tgtattaccc
     4201 caatatataa ggctaaacca ttagaagcac aacactgtta agtacggaaa ataatatcta
     4261 gtgtggtaca gttactacta ctataataca ctaatatagc tgtgggaaac tagttccaaa
     4321 gatgaattac taaccagtgt ttccaaggaa ataaatgaaa gcagagagat tagttctatt
     4381 gctagtgttt cattttcgta tatttcttac aatttctctt gttacaaata ggcactaggg
     4441 tatcaagata attttaacga ctggctgaga accctagaaa atctctgtga aaaagggatt
     4501 tgtgaaatga gagagggtaa tgtggccatt atagaaaagg cttttgtgtg ccttgcatgc
     4561 atagaccctg tgtttgatct cttaacaccc tccttgacca gaaaaagctt ctgtggatag
     4621 aaaatgatta gttatatata cttttaggga aacgtagttc tggattcttt ggttacaatt
     4681 aacagaatta agtgcaaaca aagccagaaa cctcctgata aatgagaaaa cctgcttgta
     4741 gaaggttgta aggctctgta atataggaat taggagaaaa gaaacctgtg tggtggggca
     4801 cgtctgtaat cccagcattg ggaagtagag gtagaagatt agaaatcaaa ggccagcctc
     4861 agcaacacag tgagtttgag gccaccctga actacatcag gttctgtctc ctttcttttt
     4921 tttttttttc ttttcttttt ttggtttctc tgtgtagttt tggagcctat cctggcacta
     4981 gctctgaaga gcaggctggc ctcgaactca gagatcagcc agcctctgct gggattaaag
     5041 gtatgcacca ccaacgcccc aggttttgtc tcaaacaaac aaaaataaca tcaggaggtg
     5101 gtgagagggc tcagtggtca caggcattct ctgcaaagcc tgactctgag ttggatcctt
     5161 tagagctaca tggttgaggg aagagaactg actcctggaa ggtgtcctct ggtccccaca
     5221 catagctata cacagcatgt gcattcacac acactaaata atgctatttt taaaaaaatt
     5281 aaaaacaaca acagtttggg ttgtgaaaac tagaactaga taataggtaa gaatcaagta
     5341 tcatgtaaat ttgctttcaa ctcatcccaa aatttgtttt atatttcagt ttttttcctt
     5401 cctagcttga ctgtggagtc ttgtccggaa gcaaatagtt cctttgcaga tcccacatgt
     5461 ggacaccgga cagtaggtcc tcaaatgctc cttattaggt tggttcaata atatcaattg
     5521 tttgttacta ggcagtgatg ttgtacatct ggaggagatc tcttgagccc ataatcaggt
     5581 tattaggaat aaatactcta aggctaaaaa tgtagcttag tgataagagt gcttgcctgg
     5641 tgtgctgaga ccctcggttc catctccaca accccatatt ccattacaaa ataccttttc
     5701 accgtcccta gcattaagaa acaaaacaac aaagaagttt ttctttcttc tgagatcctg
     5761 cccggagagg catttaaaac tggccagggc caaaaaaaaa aaaaaaagaa aaaaaagaaa
     5821 agaaaacagg ctagggccgg catggtggcg cacgccttta atcccagcac gcaggaggca
     5881 gaggcagggc ggatctctgt gagtttgagg tcagcctggt ctacctagtg agtttcaggg
     5941 cacccagggc taaagagact gtctcaaaaa caaaacagcc acacaatcag aaccacagca
     6001 aaacgcagtt atgatccttg gaactgtagg aatgacaagc atttaaataa taggacgagc
     6061 catttttgag aagctctgat ttcacaagtg tcagggatgg gctctgggcg agtaagattg
     6121 ctaatgctgg cctctaaatg agaccacgtg gagttgatta gattcttttc atgttcctcg
     6181 tgctctatca aataactgta cccaaataca cacacacaca cacacacaca atgcgcgcac
     6241 acacaaaatc cttttttagc ttaagaagcc cagaatcaga agtaaagcta actgtgggac
     6301 ttaagtatta ttctgaacgg aactcccagg gcgtgaagcg cgcttcaggc ttccagagaa
     6361 gcagctggcg ctggatggaa tgaaccaaga ggccagcaca ggggcagatc cgtcgagctc
     6421 tcggccaccg agctgagccc ttaggttctg gggctgggaa gggtccctag gattgtgcac
     6481 ctctcccgcg ggggacaagc agggggatgg cggggctgac gtcgggaggt ggcctccacg
     6541 ggaagggaca cccggatctc gacacagcct tggcagtgga gtcaggaagg gtagggacag
     6601 attctggacg ccctcttggc cagctcctca ccgccccacc cccgactgga gccgagagta
     6661 attcatacaa aaggagggat cgccttcgcc cctgggaatc ccagggaccg tcgctaaatt
     6721 ctggccggcc tcccagcccg gaaccgctgt gcccgcccag cgcggcggga ggagcctgcg
     6781 cctagggcgg atcgcgggtc ggcgggagag cacaagccca cagtccccgg cggtggggga
     6841 ggggcgcgct gagcgggggc ccgggagcca gcgcggggca aactgggaaa gtggcgtcgt
     6901 gtgctggctc cgccctcttc ccgagggtgg gggagaacgg tataaaagtg cggtagtcgc
     6961 gttggacgtt ctttttcgca acgggtttgc cgtcagaacg caggtgagtg gcgggtgtgg
     7021 cctccgcggg cccgggctcc ctcctttgag cggggtcggg ccgccgtgcg ggtgtcgtcg
     7081 gccgggcttc tctgcgagcg ttcccgccct ggatggcggg ctgtgcggga gggcgagggg
     7141 gggaggcctg gcggcggccc cggagcctcg cctcgtgtcg ggcgtgaggc ctagcgtggc
     7201 ttccgccccg ccgcgtgcca ccgcggccgc gctttgctgt ctgcccggct gccctcgatt
     7261 gcctgcccgc ggcccgggcc aacaaaggga gggcgtggag ctggctggta gggagccccg
     7321 tagtccgcat gtcgggcagg gagagcggca gcagtcgggg gggggaccgg gcccgcccgt
     7381 cccgcagcac atgtccaacg ccgcctggac gggtagcggc ctgtgtcctg ataaggcggc
     7441 cgggcggtgg gttttagatg ccgggttcag gtggccccgg gtcccggccc ggtctggcca
     7501 gtaccccgta gtggcttagc tccgaggagg gcgaggcccg cccgcccggc accagttgcg
     7561 tgcgcggaaa gatggccgct cccgggccct gtagcaagga gctcaaaatg gaggacgcgg
     7621 cagcccggcg gagcggggcg ggtgagtcac ccacacaaag gaagagggcc ttgcccctcg
     7681 ccggccgctg cttcctgtga ccccgtggtg taccggccgc acttcagtca ccccgggcgc
     7741 tctttcggag caccgctggc ctccgctggg ggaggggatc tgtctaatgg cgttggagtt
     7801 tgctcacatt tggtgggtgg agactgtagc caggccagcc tggccatgaa agtaattctt
     7861 ggaatttgcc cattttgagt ttggagcgaa gctgattgac aaagctgctt agccgttcaa
     7921 aggtattctt cgaacttttt ttttaaggtg ttgtgaaaac ctcgaggtac gtatcgcgaa
     7981 agctagcata ttacccctaa cacctgccac cccagtctta atcagtggtg gaagaacggt
     8041 ctcagaactg tttgtctcaa ttggccattt aagtttaata gtgaaagact ggttaatgat
     8101 aacaatgcat cggaaaacct tcaggaggaa aggagaatgt tttgtggaac atttttgtgt
     8161 gtgtggcagt tttaagttat tagttttcaa aatcagtact ttttaatgga aacaacttga
     8221 ccaaaaatct gtcacagaat tttgagaccc attaaaatac aagtttaatg agaagtctgt
     8281 ctctgttaat gctgaagtca ttactaagtg cttagcttag caaggtatgt ggatgcccat
     8341 ttgtgttcca agggattgga ctgttcatca ggacccagag ctgagtttca agggctcaag
     8401 agatggctta ttacctgtgg gtgtcttgaa ggttctggtt gggacaaatt aggaatgttt
     8461 ttggcagaca tggtgactac ccttcatctg ggtgagttca gttgatttgt cttgagcctt
     8521 tggggtttac acaagtaaat gacatcatac agttagtgta ttgttagtga atattaatat
     8581 atgaggcagg ctttgctcta gcaattttag aactagtttt caggaaaggg ttcatcttgt
     8641 gcattggatg tctgattcta tcacttagag tttaaactga aagtgctcaa gaggttttat
     8701 ttaggctggg atataaataa gcctttctgt agcttgtaat ggtatcagga atttaaaagg
     8761 ccatctgggg cacaaagatt aagcagaaaa ggtagaaggt gagattgggg gactttgagt
     8821 acttcacaca ctttaatgtg tgggtgcttt agtgcatata gtacaactgc cagataaggg
     8881 catccacatc tgattgtttg gaaggcacct tgtggtttct gggaattcag aattgggaga
     8941 aaaagtgctc ccaaccgctg aagcccttgg taatctgcag ggtgtttatt tagcaggaga
     9001 taaggacaaa aagttatagt gtggagttgg ttgagttggt agatgtcatt acaacaggtg
     9061 gtcttaaatt gggttaggag tcactttgaa atacctgggc cataagcaaa gtggcatttt
     9121 cacctttcag gagaaactgg tacacttatc cattctatag tgcatgcttg ttcaattggg
     9181 ctgatgacta aaccggtgac taaaggtttg tcagtataaa tggatgggtt gtaggcagac
     9241 ggtgaggaat tactatacct gcaaggagtc attgcctgat ctgcctggaa aggggcagga
     9301 ttgagtctca gaaggtgtac accataggat atggaaaaat ttgtcacgcc tagcattcaa
     9361 cttagtggtg tagcgccacc tactggcact ttaaaagctt agcatagagg agcatgtgtg
     9421 ttaggagctc ggatgggatc cagggcctca aggtttgcat gtaaataaaa gccctttacc
     9481 aaattaacta cataccagca tacatcagtc ctttagtgtt gaaaaacaga agtgaaagct
     9541 aatatatata gtgcttgctt tatttaagtc tagctgatta cgtgtttggt tgccagtgtg
     9601 actagtctgg agttgaattt gtcctcagac acgtaaaatg gaatttggga ttcacaacac
     9661 tctagtatga gggacctaat ggcctgtacc aggcacaaac gtgtctataa actacacaaa
     9721 acgaaggaat ttacaggaat taggaaggta ttcttaacat taaaacatta tgggcatttt
     9781 aaaaaaagct ttgacaggat ttctttgtca tggctgtcct ggagctagtt gtgtagacca
     9841 ggctgggctg aaatcttgtc tgcctgcctg gcttggacac ttttttatta tgtatacaac
     9901 attctgcttc catgtatatc tgcacattag aagacggcac cagatctcct aatggatggt
     9961 tgtgagccac catgtggttg ctgggaattg aactcaggac ctctggaaga gcagtgctct
    10021 taacctctga gccatctcca gccccagctt gggcacattt ttaatggctg ggaaatcaaa
    10081 ccccctaggc cttctgtcag taatgaaggg cttttggcta ccgagagtag gatttaaggt
    10141 tattcggagc tgcaggtctg cctcagtgca ggtttgggag tccagcatct tagaaaatgc
    10201 agtgaagcca agctgagcta tattttgttt aaaaaaaaaa taagtgggta aagtgctgct
    10261 gagcctgatg accaagctgg gacacaagta gaagaacata ggccaatgct ctatattaaa
    10321 agcatgggtc atttttaatg ctcttgagaa ggctatgcct acactactct cagccaccgc
    10381 agcgtgttta aattaaacta gtttggaaat tttctttggg ggtaagctat ttaacctagt
    10441 gccttggcag gtatactact gaactctcct cctcattcct ttttgttttt taagaatttc
    10501 agtcaggctc aggcagccct taaacttgtg attaagcctg agaacagtta cgattatgag
    10561 cctattagta taccgatcaa tatgtgaatt tttttgggat gggggtcagg cctccctgcc
    10621 tcccaaatac tgggactaaa ggctgcacca ccacaacctg gctcttgaaa tacttttcta
    10681 cattttttgg ggggcatggg tgggagagca gggtttctct gtattagccc tggctctcct
    10741 ggaactctgt agaccaggct attcttgagc tcagattagc ctgtctctgc ctcctaaatt
    10801 ctgggattaa aggtgtgtgc tactgctgcc tggctacaaa gacatttttt tttttcttaa
    10861 atttaaaaac aaaagtggtt cttttagaag ggtggttggt gttggcacat actccaagca
    10921 ctcaggtttt gagtttgtcc caggaatgaa gactgcatta ctgccgcccc tccctggtaa
    10981 gggctacaca gagaaatcct atttggagcc tgtcctggta actcgctctg tagaccaggc
    11041 tggcctcgaa ctcaagagaa ccacctgcct ctgaatgctg gtattaaggg caggcaccac
    11101 caacacccag cctaaaaaat gtcttttttt taaagatttt tttttttttt ttacagaata
    11161 aacattctgt ttacaatatt ctgcttctat gtatatctgc acactagaag agggcacccg
    11221 atctcataat ggatggttgt gagccaccaa gtggttgctg ggaattgaac tcagaacctc
    11281 tggaagagca gtcagtgctc ttaacctctg agccatctct ccagccccta aaaatggctc
    11341 ttgagatagg gtctcaagta gtttgagact gagttggcta tataaacaag gctggcacat
    11401 agcaccatgt acagctgggt ttagtttaca tggggtgttt ttgtctctgg aggcaggagg
    11461 atcatttgag catagggagt taatagtgag gtcatgtttt atctactctt ctgaattgag
    11521 aactaagctg atgcaaagca agtttgactg aagaagtcca gtttatgaga acaagggtgg
    11581 aaactaatgt gtcaaagatg gccttgcatg tgttttagat gatgacccag tcacttggga
    11641 attactggat gtgtaagacc tatatcttga caggagtgaa cagtgtctta taggtcctat
    11701 atgaaagaaa tgagacatac ccattttgtt tcccctaaga attcactttt cctaacctgg
    11761 ttcatgctat ttaggttatt ttacttgcaa atcctagggt gctcccttac ccagtattgc
    11821 ttatgtggca ccaaagtcac tcactcccat gatttgcaag tctctgggaa cttccatgac
    11881 aacctagaat agcaactcaa atacattttc tcagtaccaa ttttgaagaa aaaatatttt
    11941 gcaaaatagc tgtatggatg ggtactaaat agtgaggtta tctccagaag gcctatgaag
    12001 aattaaggtt gagttcagtt gagttcagca gcaagtttaa ggttcatcca tttttgtaca
    12061 gtgttttcct attacggtaa gtgttttgcc tgcaggaata tctgtaccac atgcttgcct
    12121 ggtacctata tcggccagaa gagggctttg gatcctctgg acttgaatta cagatgggta
    12181 ttagccacca tttaggtgct gggaattgaa accaagtcct ctggaagaac agcaagtgac
    12241 tcgacgtccg gagctctggc gcgcctgtgg aat
//
