LOCUS       sequence-337733-       12441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.2-Hygro
FEATURES             Location/Qualifiers
     source          1..12441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8724..8744)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8729..9058
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8909..9044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8971..8990
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9107..10132
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9107..10132
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10262..10383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10299..10318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10353..10372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10434..10451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10605..11193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10685..10704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11364..12155,12156..12224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11987..12006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12225..12329)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgcagccc ccggagcccg cggaccccga ccccccgccg ctcgcccgcc gttgcgccgc
       61 tctctgcggg ggggctaggg ggccgcgggg ggaaggccac gccccctcca ctttttccag
      121 gaatgcgcgg catgacccgc cccccccatg acccgccaac acccccccga tgccccccgg
      181 gggtctttcc tggggggcct ttgtcagggt tgcctgtgtc acccacgggc cccttggcca
      241 cgcttgcctg ggtcaccccg gagccccggg tcacgtttgc cggggtcagc cctggggcgc
      301 ttcttcacgc tccattgcca tgcagaattc actagtgatt agaatcccac gttgtgcata
      361 gaaacagatg caggcaaaac atccacacat ataaaacaaa aaattaaaac caataaaact
      421 cctaaacttt tggtctttct tgaatcttca atccctcagg ttatgaaata atcatttatg
      481 cagtcaaaaa tttgccattc ttgttgccag gtgtggtgat gattcgggga agcagaagca
      541 ggcagatctc tgtgaatgag gccagcctgg tctacaaagt gagtcccagg acagtcaggc
      601 ctgttacaca gagaaacctt gaaaaaaaaa agataatatg tactgttgta ttaccccaat
      661 atataaggct aaaccattag aagcacaaca ctgttaagta cggaaaataa tatctagtgt
      721 ggtacagtta ctactactat aatacactaa tatagctgtg ggaaactagt tccaaagatg
      781 aattactaac cagtgtttcc aaggaaataa atgaaagcag agagattagt tctattgcta
      841 gtgtttcatt ttcgtatatt tcttacaatt tctcttgtta caaataggca ctagggtatc
      901 aagataattt taacgactgg ctgagaaccc tagaaaatct ctgtgaaaaa gggatttgtg
      961 aaatgagaga gggtaatgtg gccattatag aaaaggcttt tgtgtgcctt gcatgcatag
     1021 accctgtgtt tgatctctta acaccctcct tgaccagaaa aagcttctgt ggatagaaaa
     1081 tgattagtta tatatacttt tagggaaacg tagttctgga ttctttggtt acaattaaca
     1141 gaattaagtg caaacaaagc cagaaacctc ctgataaatg agaaaacctg cttgtagaag
     1201 gttgtaaggc tctgtaatat aggaattagg agaaaagaaa cctgtgtggt ggggcacgtc
     1261 tgtaatccca gcattgggaa gtagaggtag aagattagaa atcaaaggcc agcctcagca
     1321 acacagtgag tttgaggcca ccctgaacta catcaggttc tgtctccttt cttttttttt
     1381 tttttctttt ctttttttgg tttctctgtg tagttttgga gcctatcctg gcactagctc
     1441 tgaagagcag gctggcctcg aactcagaga tcagccagcc tctgctggga ttaaaggtat
     1501 gcaccaccaa cgccccaggt tttgtctcaa acaaacaaaa ataacatcag gaggtggtga
     1561 gagggctcag tggtcacagg cattctctgc aaagcctgac tctgagttgg atcctttaga
     1621 gctacatggt tgagggaaga gaactgactc ctggaaggtg tcctctggtc cccacacata
     1681 gctatacaca gcatgtgcat tcacacacac taaataatgc tatttttaaa aaaattaaaa
     1741 acaacaacag tttgggttgt gaaaactaga actagataat aggtaagaat caagtatcat
     1801 gtaaatttgc tttcaactca tcccaaaatt tgttttatat ttcagttttt ttccttccta
     1861 gcttgactgt ggagtcttgt ccggaagcaa atagttcctt tgcagatccc acatgtggac
     1921 accggacagt aggtcctcaa atgctcctta ttaggttggt tcaataatat caattgtttg
     1981 ttactaggca gtgatgttgt acatctggag gagatctctt gagcccataa tcaggttatt
     2041 aggaataaat actctaaggc taaaaatgta gcttagtgat aagagtgctt gcctggtgtg
     2101 ctgagaccct cggttccatc tccacaaccc catattccat tacaaaatac cttttcaccg
     2161 tccctagcat taagaaacaa aacaacaaag aagtttttct ttcttctgag atcctgcccg
     2221 gagaggcatt taaaactggc cagggccaaa aaaaaaaaaa aaagaaaaaa aagaaaagaa
     2281 aacaggctag ggccggcatg gtggcgcacg cctttaatcc cagcacgcag gaggcagagg
     2341 cagggcggat ctctgtgagt ttgaggtcag cctggtctac ctagtgagtt tcagggcacc
     2401 cagggctaaa gagactgtct caaaaacaaa acagccacac aatcagaacc acagcaaaac
     2461 gcagttatga tccttggaac tgtaggaatg acaagcattt aaataatagg acgagccatt
     2521 tttgagaagc tctgatttca caagtgtcag ggatgggctc tgggcgagta agattgctaa
     2581 tgctggcctc taaatgagac cacgtggagt tgattagatt cttttcatgt tcctcgtgct
     2641 ctatcaaata actgtaccca aatacacaca cacacacaca cacacaatgc gcgcacacac
     2701 aaaatccttt tttagcttaa gaagcccaga atcagaagta aagctaactg tgggacttaa
     2761 gtattattct gaacggaact cccagggcgt gaagcgcgct tcaggcttcc agagaagcag
     2821 ctggcgctgg atggaatgaa ccaagaggcc agcacagggg cagatccgtc gagctctcgg
     2881 ccaccgagct gagcccttag gttctggggc tgggaagggt ccctaggatt gtgcacctct
     2941 cccgcggggg acaagcaggg ggatggcggg gctgacgtcg ggaggtggcc tccacgggaa
     3001 gggacacccg gatctcgaca cagccttggc agtggagtca ggaagggtag ggacagattc
     3061 tggacgccct cttggccagc tcctcaccgc cccacccccg actggagccg agagtaattc
     3121 atacaaaagg agggatcgcc ttcgcccctg ggaatcccag ggaccgtcgc taaattctgg
     3181 ccggcctccc agcccggaac cgctgtgccc gcccagcgcg gcgggaggag cctgcgccta
     3241 gggcggatcg cgggtcggcg ggagagcaca agcccacagt ccccggcggt gggggagggg
     3301 cgcgctgagc gggggcccgg gagccagcgc ggggcaaact gggaaagtgg cgtcgtgtgc
     3361 tggctccgcc ctcttcccga gggtggggga gaacggtata aaagtgcggt agtcgcgttg
     3421 gacgttcttt ttcgcaacgg gtttgccgtc agaacgcagg tgagtggcgg gtgtggcctc
     3481 cgcgggcccg ggctccctcc tttgagcggg gtcgggccgc cgtgcgggtg tcgtcggccg
     3541 ggcttctctg cgagcgttcc cgccctggat ggcgggctgt gcgggagggc gaggggggga
     3601 ggcctggcgg cggccccgga gcctcgcctc gtgtcgggcg tgaggcctag cgtggcttcc
     3661 gccccgccgc gtgccaccgc ggccgcgctt tgctgtctgc ccggctgccc tcgattgcct
     3721 gcccgcggcc cgggccaaca aagggagggc gtggagctgg ctggtaggga gccccgtagt
     3781 ccgcatgtcg ggcagggaga gcggcagcag tcgggggggg gaccgggccc gcccgtcccg
     3841 cagcacatgt ccaacgccgc ctggacgggt agcggcctgt gtcctgataa ggcggccggg
     3901 cggtgggttt tagatgccgg gttcaggtgg ccccgggtcc cggcccggtc tggccagtac
     3961 cccgtagtgg cttagctccg aggagggcga ggcccgcccg cccggcacca gttgcgtgcg
     4021 cggaaagatg gccgctcccg ggccctgtag caaggagctc aaaatggagg acgcggcagc
     4081 ccggcggagc ggggcgggtg agtcacccac acaaaggaag agggccttgc ccctcgccgg
     4141 ccgctgcttc ctgtgacccc gtggtgtacc ggccgcactt cagtcacccc gggcgctctt
     4201 tcggagcacc gctggcctcc gctgggggag gggatctgtc taatggcgtt ggagtttgct
     4261 cacatttggt gggtggagac tgtagccagg ccagcctggc catgaaagta attcttggaa
     4321 tttgcccatt ttgagtttgg agcgaagctg attgacaaag ctgcttagcc gttcaaaggt
     4381 attcttcgaa cttttttttt aaggtgttgt gaaaacctcg aggtacgtat cgcgaaagct
     4441 agcatattac ccctaacacc tgccacccca gtcttaatca gtggtggaag aacggtctca
     4501 gaactgtttg tctcaattgg ccatttaagt ttaatagtga aagactggtt aatgataaca
     4561 atgcatcgga aaaccttcag gaggaaagga gaatgttttg tggaacattt ttgtgtgtgt
     4621 ggcagtttta agttattagt tttcaaaatc agtacttttt aatggaaaca acttgaccaa
     4681 aaatctgtca cagaattttg agacccatta aaatacaagt ttaatgagaa gtctgtctct
     4741 gttaatgctg aagtcattac taagtgctta gcttagcaag gtatgtggat gcccatttgt
     4801 gttccaaggg attggactgt tcatcaggac ccagagctga gtttcaaggg ctcaagagat
     4861 ggcttattac ctgtgggtgt cttgaaggtt ctggttggga caaattagga atgtttttgg
     4921 cagacatggt gactaccctt catctgggtg agttcagttg atttgtcttg agcctttggg
     4981 gtttacacaa gtaaatgaca tcatacagtt agtgtattgt tagtgaatat taatatatga
     5041 ggcaggcttt gctctagcaa ttttagaact agttttcagg aaagggttca tcttgtgcat
     5101 tggatgtctg attctatcac ttagagttta aactgaaagt gctcaagagg ttttatttag
     5161 gctgggatat aaataagcct ttctgtagct tgtaatggta tcaggaattt aaaaggccat
     5221 ctggggcaca aagattaagc agaaaaggta gaaggtgaga ttgggggact ttgagtactt
     5281 cacacacttt aatgtgtggg tgctttagtg catatagtac aactgccaga taagggcatc
     5341 cacatctgat tgtttggaag gcaccttgtg gtttctggga attcagaatt gggagaaaaa
     5401 gtgctcccaa ccgctgaagc ccttggtaat ctgcagggtg tttatttagc aggagataag
     5461 gacaaaaagt tatagtgtgg agttggttga gttggtagat gtcattacaa caggtggtct
     5521 taaattgggt taggagtcac tttgaaatac ctgggccata agcaaagtgg cattttcacc
     5581 tttcaggaga aactggtaca cttatccatt ctatagtgca tgcttgttca attgggctga
     5641 tgactaaacc ggtgactaaa ggtttgtcag tataaatgga tgggttgtag gcagacggtg
     5701 aggaattact atacctgcaa ggagtcattg cctgatctgc ctggaaaggg gcaggattga
     5761 gtctcagaag gtgtacacca taggatatgg aaaaatttgt cacgcctagc attcaactta
     5821 gtggtgtagc gccacctact ggcactttaa aagcttagca tagaggagca tgtgtgttag
     5881 gagctcggat gggatccagg gcctcaaggt ttgcatgtaa ataaaagccc tttaccaaat
     5941 taactacata ccagcataca tcagtccttt agtgttgaaa aacagaagtg aaagctaata
     6001 tatatagtgc ttgctttatt taagtctagc tgattacgtg tttggttgcc agtgtgacta
     6061 gtctggagtt gaatttgtcc tcagacacgt aaaatggaat ttgggattca caacactcta
     6121 gtatgaggga cctaatggcc tgtaccaggc acaaacgtgt ctataaacta cacaaaacga
     6181 aggaatttac aggaattagg aaggtattct taacattaaa acattatggg cattttaaaa
     6241 aaagctttga caggatttct ttgtcatggc tgtcctggag ctagttgtgt agaccaggct
     6301 gggctgaaat cttgtctgcc tgcctggctt ggacactttt ttattatgta tacaacattc
     6361 tgcttccatg tatatctgca cattagaaga cggcaccaga tctcctaatg gatggttgtg
     6421 agccaccatg tggttgctgg gaattgaact caggacctct ggaagagcag tgctcttaac
     6481 ctctgagcca tctccagccc cagcttgggc acatttttaa tggctgggaa atcaaacccc
     6541 ctaggccttc tgtcagtaat gaagggcttt tggctaccga gagtaggatt taaggttatt
     6601 cggagctgca ggtctgcctc agtgcaggtt tgggagtcca gcatcttaga aaatgcagtg
     6661 aagccaagct gagctatatt ttgtttaaaa aaaaaataag tgggtaaagt gctgctgagc
     6721 ctgatgacca agctgggaca caagtagaag aacataggcc aatgctctat attaaaagca
     6781 tgggtcattt ttaatgctct tgagaaggct atgcctacac tactctcagc caccgcagcg
     6841 tgtttaaatt aaactagttt ggaaattttc tttgggggta agctatttaa cctagtgcct
     6901 tggcaggtat actactgaac tctcctcctc attccttttt gttttttaag aatttcagtc
     6961 aggctcaggc agcccttaaa cttgtgatta agcctgagaa cagttacgat tatgagccta
     7021 ttagtatacc gatcaatatg tgaatttttt tgggatgggg gtcaggcctc cctgcctccc
     7081 aaatactggg actaaaggct gcaccaccac aacctggctc ttgaaatact tttctacatt
     7141 ttttgggggg catgggtggg agagcagggt ttctctgtat tagccctggc tctcctggaa
     7201 ctctgtagac caggctattc ttgagctcag attagcctgt ctctgcctcc taaattctgg
     7261 gattaaaggt gtgtgctact gctgcctggc tacaaagaca tttttttttt tcttaaattt
     7321 aaaaacaaaa gtggttcttt tagaagggtg gttggtgttg gcacatactc caagcactca
     7381 ggttttgagt ttgtcccagg aatgaagact gcattactgc cgcccctccc tggtaagggc
     7441 tacacagaga aatcctattt ggagcctgtc ctggtaactc gctctgtaga ccaggctggc
     7501 ctcgaactca agagaaccac ctgcctctga atgctggtat taagggcagg caccaccaac
     7561 acccagccta aaaaatgtct tttttttaaa gatttttttt ttttttttac agaataaaca
     7621 ttctgtttac aatattctgc ttctatgtat atctgcacac tagaagaggg cacccgatct
     7681 cataatggat ggttgtgagc caccaagtgg ttgctgggaa ttgaactcag aacctctgga
     7741 agagcagtca gtgctcttaa cctctgagcc atctctccag cccctaaaaa tggctcttga
     7801 gatagggtct caagtagttt gagactgagt tggctatata aacaaggctg gcacatagca
     7861 ccatgtacag ctgggtttag tttacatggg gtgtttttgt ctctggaggc aggaggatca
     7921 tttgagcata gggagttaat agtgaggtca tgttttatct actcttctga attgagaact
     7981 aagctgatgc aaagcaagtt tgactgaaga agtccagttt atgagaacaa gggtggaaac
     8041 taatgtgtca aagatggcct tgcatgtgtt ttagatgatg acccagtcac ttgggaatta
     8101 ctggatgtgt aagacctata tcttgacagg agtgaacagt gtcttatagg tcctatatga
     8161 aagaaatgag acatacccat tttgtttccc ctaagaattc acttttccta acctggttca
     8221 tgctatttag gttattttac ttgcaaatcc tagggtgctc ccttacccag tattgcttat
     8281 gtggcaccaa agtcactcac tcccatgatt tgcaagtctc tgggaacttc catgacaacc
     8341 tagaatagca actcaaatac attttctcag taccaatttt gaagaaaaaa tattttgcaa
     8401 aatagctgta tggatgggta ctaaatagtg aggttatctc cagaaggcct atgaagaatt
     8461 aaggttgagt tcagttgagt tcagcagcaa gtttaaggtt catccatttt tgtacagtgt
     8521 tttcctatta cggtaagtgt tttgcctgca ggaatatctg taccacatgc ttgcctggta
     8581 cctatatcgg ccagaagagg gctttggatc ctctggactt gaattacaga tgggtattag
     8641 ccaccattta ggtgctggga attgaaacca agtcctctgg aagaacagca agtgactcga
     8701 cgtccggagc tctggcgcgc cgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8761 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8881 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9061 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga
     9121 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     9181 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     9241 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     9301 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     9361 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     9421 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     9481 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     9541 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     9601 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     9661 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     9721 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     9781 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     9841 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     9901 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     9961 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
    10021 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
    10081 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct
    10141 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10201 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10261 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10381 ttatcatgtc tgtataccgt cgacctctag gcgcgccatg gctcttccgc ttcctcgctc
    10441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11041 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcatct
    12361 catcacggtc acgcatggct gcgcccgcgc agcgcccccc gacccccccc cgatccccgg
    12421 aacgccagcc gccatccacc g
//
