LOCUS       sequence-337739-        3977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensing Device with Reporter protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang
            MW
  TITLE     Engineering microbes to sense and eradicate Pseudomonas aeruginosa, 
            a human pathogen.
  JOURNAL   Mol Syst Biol. 2011 Aug 16;7:521. doi: 10.1038/msb.2011.55.
  PUBMED    21847113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-08-16"; volume: "7:521. doi"; pages: " 
            10.1038/msb.2011.55"
COMMENT     Sequence Label: pTetR-LasR-pLuxR-GFP
FEATURES             Location/Qualifiers
     source          1..3977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      83..154
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      170..197
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    204..224
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(230..247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(412..1000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(492..511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1171..1962,1963..2031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1794..1813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2032..2136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2204..2222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2226..2269)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2272..2293
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    2294..2312
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2319..2337
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2356..2367
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3113..3184
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3200..3227
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             join(3324..3977,1..60)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3354..3382)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     join(3977,1..20)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
       61 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      121 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      181 gggtgggcct ttctgcgttt atatactagt agcggccgct gcaggcttcc tcgctcactg
      241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2161 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2221 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
     2281 gccgcttcta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
     2341 tgagcactac tagagaaaga ggagaaatac tagatggcct tggttgacgg ttttcttgag
     2401 ctggaacgct caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac
     2461 cttggattct cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac
     2521 gccttcatcg tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac
     2581 gcgcgggtcg acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa
     2641 ccgtccatct accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc
     2701 ctggtgtatg ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc
     2761 ctcagcgtgg aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg
     2821 accctgtgga tgctcaagga ctacgcactg caaagcggtg ccggactggc cttcgaacat
     2881 ccggtcagca aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc
     2941 atcggcaaga ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac
     3001 ttccatatgg gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt
     3061 atggccgtta atttgggtct tattactctc taataatact agagccaggc atcaaataaa
     3121 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3181 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     3241 gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact
     3301 agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc
     3361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     3421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     3481 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     3541 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     3601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     3661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     3721 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     3781 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     3841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     3901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     3961 aaaagagaga ccacatg
//
