LOCUS       sequence-337757-        7029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Mauger DM, Lin C, Garcia-Blanco MA
  TITLE     hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth 
            factor receptor 2 exon IIIc.
  JOURNAL   Mol Cell Biol. 2008 Sep . 28(17):5403-19.
  PUBMED    18573884
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Sep . 28(17):5403-19."
COMMENT     Sequence Label: p3x-FLAG-hnRNPH1
FEATURES             Location/Qualifiers
     source          1..7029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(253..631)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     578..597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(816..920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      947..1402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1034..1053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1244..1265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1528..1550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1542..1559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1543..1559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1720..2099
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2100..2303
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2254..2274
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2333..2398
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3768..4390
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3967..3986)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4414..4434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4432..4729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4783..5577
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4837..4856)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5447..5466
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6229..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6266..6285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6320..6339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6368..6387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6369..6387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6401..6417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6401..6417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6414..6436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6425..6441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6449..6455,6456..6473,6474..6479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6494..6515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6632..6649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6883..6902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttccagct cttggttgtg aaccagaagt ggcagcggcc atacttcctc ctgttggagc
       61 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      121 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      181 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      241 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      301 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      361 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      421 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      481 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      541 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      601 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      661 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      721 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      781 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      841 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      901 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
      961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1381 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     1441 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1501 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     1561 ccaagctgat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     1621 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     1681 acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt
     1741 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1801 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1861 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1921 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     1981 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2041 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2101 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     2161 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2221 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     2281 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gaattaacca tggactacaa
     2341 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct
     2401 tgcggccgcg atgatgttgg gcacggaagg tggagaggga ttcgtggtga aggtccgggg
     2461 cttgccctgg tcttgctcgg ccgatgaagt gcagaggttt ttttctgact gcaaaattca
     2521 aaatggggct caaggtattc gtttcatcta caccagagaa ggcagaccaa gtggcgaggc
     2581 ttttgttgaa cttgaatcag aagatgaagt caaattggcc ctgaaaaaag acagagaaac
     2641 tatgggacac agatatgttg aagtattcaa gtcaaacaac gttgaaatgg attgggtgtt
     2701 gaagcatact ggtccaaata gtcctgacac ggccaatgat ggctttgtac ggcttagagg
     2761 acttcccttt ggatgtagca aggaagaaat tgttcagttc ttctcagggt tggaaatcgt
     2821 gccaaatggg ataacattgc cggtggactt ccaggggagg agtacggggg aggccttcgt
     2881 gcagtttgct tcacaggaaa tagctgaaaa ggctctaaag aaacacaagg aaagaatagg
     2941 gcacaggtat attgaaatct ttaagagcag tagagctgaa gttagaactc attatgatcc
     3001 accacgaaag cttatggcca tgcagcggcc aggtccttat gacagacctg gggctggtag
     3061 agggtataac agcattggca gaggagctgg ctttgagagg atgaggcgtg gtgcttatgg
     3121 tggaggctat ggaggctatg atgattacaa tggctataat gatggctatg gatttgggtc
     3181 agatagattt ggaagagacc tcaattactg tttttcagga atgtctgatc acagatacgg
     3241 ggatggtggc tctactttcc agagcacaac aggacactgt gtacacatgc ggggattacc
     3301 ttacagagct actgagaatg acatttataa ttttttttca ccgctcaacc ctgtgagagt
     3361 acacattgaa attggtcctg atggcagagt aactggtgaa gcagatgtcg agttcgcaac
     3421 tcatgaagat gctgtggcag ctatgtcaaa agacaaagca aatatgcaac acagatatgt
     3481 agaactcttc ttgaattcta cagcaggagc aagcggtggt gcttacgaac acagatatgt
     3541 agaactcttc ttgaattcta cagcaggagc aagcggtggt gcttatggta gccaaatgat
     3601 gggaggcatg ggcttgtcaa accagtccag ctacgggggc ccagccagcc agcagctgag
     3661 tgggggttac ggaggcggct acggtggcca gagcagcatg agtggatacg accaagtttt
     3721 acaggaaaac tccagtgatt ttcaatcaaa cattgcatag ggatcccggg tggcatccct
     3781 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3841 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3901 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3961 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     4021 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     4081 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     4141 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     4201 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac
     4261 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg
     4321 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc
     4381 ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa
     4441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4681 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggaattgatc
     4741 agcttgggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4801 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4861 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4921 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     4981 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5041 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5101 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5161 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5221 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5281 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5341 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5401 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5461 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     5521 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     5581 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5641 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5701 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc gggctcgatc
     5761 ccctcgcgag ttggttcagc tgctgcctga ggctggacga cctcgcggag ttctaccggc
     5821 agtgcaaatc cgtcggcatc caggaaacca gcagcggcta tccgcgcatc catgcccccg
     5881 aactgcagga gtggggaggc acgatggccg ctttggtcga cccggacggg acgctcctgc
     5941 gcctgataca gaacgaattg cttgcaggca tctcatgagt gtgtcttccc gttttccgcc
     6001 tgaggtcact gcgtggatgg agcgctggcg cctgctgcgc gacggcgagc tgctcaccac
     6061 ccactcgcca agctggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6121 ccgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     6181 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     6241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6361 atcaattccc tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt
     6421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7021 gttcggtgt
//
