LOCUS       sequence-337801-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of mitochondria-targeted Green Fluorescent 
            Protein under control of the EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kole K, Voesenek BJB, Brinia ME, Petersen N, Kole MHP
  TITLE     Parvalbumin basket cell myelination accumulates axonal mitochondria 
            to internodes.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7598. doi: 10.1038/s41467-022-35350-x.
  PUBMED    36494349
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7598. doi"; pages: " 
            10.1038/s41467-022-35350-x"
COMMENT     Sequence Label: pAAV-EF1a-mitoGFP-DIO
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(57..773)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(100..120)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     715..743
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(774..800)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(837..908)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccggaagtct ggacatttat
       61 ttgtatagtt catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc
      121 atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg
      181 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atgatctgct
      241 agttgaacgc ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc
      301 ttttgtttgt ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt
      361 ccaagaatgt ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg
      421 gtatcacctt caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat
      481 atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc
      541 atatgatctg ggtatcttga aaagcattga acaccataag agaaagtagt gacaagtgtt
      601 ggccatggaa caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat
      661 gttgcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct
      721 aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttact cattaagctt
      781 gcataatcag gaacatcata atcgataagc ttcccctccg gcggcaacga atggatcttg
      841 gcgcgcggca ctgggagccg ccgggccgag cctgtcaagc cccgcagcag cagcggcgtc
      901 aggacggaca
//
