LOCUS       sequence-337809-        8411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of C-tag TNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Pinci F, Gaidt MM, Jung C, Kuut G, Jackson MA, Bauernfried S, 
            Hornung V
  TITLE     C-tag TNF: a reporter system to study TNF shedding.
  JOURNAL   J Biol Chem. 2020 Dec 25;295(52):18065-18075. doi: 
            10.1074/jbc.RA120.015248. Epub 2020 Oct 20.
  PUBMED    33082141
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA120.015248"; journalName: "J Biol Chem"; date: 
            "2020-12-25- 25"; volume: "295"; issue: "52"; pages: "18065-18075"
COMMENT     Sequence Label: pLI_BFP_C-tag TNF
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        172..486
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     459..483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1211..1721
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1640..1658
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1731..2429
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             2430..2483
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2484..3101,3102..3107,3108..3143,3144..3146,3147..3182,
                     3183..3185,3186..3221,3222..3230)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3270..3290)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3688..3699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3904..4137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4209..4330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4246..4265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4300..4319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4370..4505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4505..4576
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4577..4645,4646..5437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4795..4814)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5608..6196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6097..6116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6350..6367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6484..6505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6520..6525,6526..6543,6544..6550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6558..6574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6563..6585
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6582..6598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6582..6598
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6617..6637
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6619..6637
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6665..6891
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6892..7072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7119..7244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7737..7970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8155..8199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      481 atcgcctgga gaattggcta gcatgagcac tgaaagcatg atccgggacg tggagctggc
      541 cgaggaggcg ctccccaaga agacaggggg gccccagggc tccaggcggt gcttgttcct
      601 cagcctcttc tccttcctga tcgtggcagg cgccaccacg ctcttctgcc tgctgcactt
      661 tggagtgatc ggcccccaga gggaagagtt ccccagggac ctctctctaa tcagccctga
      721 gccggaagct gtcagatcat cttctcgaac cccgagtgac aagcctgtag cccatgttgt
      781 agcaaaccct caagctgagg ggcagctcca gtggctgaac cgccgggcca atgccctcct
      841 ggccaatggc gtggagctga gagataacca gctggtggtg ccatcagagg gcctgtacct
      901 catctactcc caggtcctct tcaagggcca aggctgcccc tccacccatg tgctcctcac
      961 ccacaccatc agccgcatcg ccgtctccta ccagaccaag gtcaacctcc tctctgccat
     1021 caagagcccc tgccagaggg agaccccaga gggggctgag gccaagccct ggtatgagcc
     1081 catctatctg ggaggggtct tccagctgga gaagggtgac cgactcagcg ctgagatcaa
     1141 tcggcccgac tatctcgact ttgccgagtc tgggcaggtc tactttggga tcattgccct
     1201 gtgaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     1261 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     1321 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     1381 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     1441 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     1501 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     1561 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     1621 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     1681 tcggctccct cgttgaccga atcaccgacc tctctcccca gcaattcacc atgagcgagc
     1741 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact
     1801 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     1861 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct
     1921 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct
     1981 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     2041 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     2101 tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca
     2161 ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc
     2221 tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg
     2281 ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg
     2341 aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc
     2401 tccctagcaa actggggcac aaacttaatg aaggtagagg ttctctcctc acttgtggtg
     2461 atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag
     2521 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     2581 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     2641 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     2701 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     2761 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     2821 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     2881 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     2941 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     3001 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     3061 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     3121 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     3181 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     3241 atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3301 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3361 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3421 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3481 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3541 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3601 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     3661 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3721 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3781 atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt
     3841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3901 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4141 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4201 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4321 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4381 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4441 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4501 aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4561 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4621 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4681 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4741 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4801 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4861 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4921 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4981 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5041 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5101 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5161 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5221 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5281 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5341 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5401 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5461 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5521 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5581 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5641 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5701 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5761 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5821 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5881 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5941 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6001 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6061 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6121 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6181 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6241 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6301 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6361 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6421 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6481 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6541 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6601 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     6661 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     6721 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     6781 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     6841 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     6901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7081 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7141 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7201 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7261 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7321 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7381 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7441 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7501 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7561 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     7621 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     7681 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     7741 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     7801 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     7861 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     7921 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     7981 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8041 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8101 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8161 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8221 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8281 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8341 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     8401 ttcatccaca a
//
