LOCUS       sequence-337830-       10902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  deletion of a genomic locus in Blimp1(Prdm1) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Aramaki S, Kagiwada S, Wu G, Obridge D, Adachi K, Kutejova E, 
            Lickert H, Hubner K, Scholer HR
  TITLE     Residual pluripotency is required for inductive germ cell 
            segregation.
  JOURNAL   EMBO Rep. 2021 Aug 4;22(8):e52553. doi: 10.15252/embr.202152553. 
            Epub 2021 Jun 22.
  PUBMED    34156139
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-08-4"; volume: "22(8):e52553. doi"; pages: " 
            10.15252/embr.202152553. Epub 2021 Jun 22"
COMMENT     Sequence Label: pX330A-Blimp1-L1-#2
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..390
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      402..411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             411..476
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             483..503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             528..4628
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             528..4628
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4629..4676
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4704..4721)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4710..4917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4926..5066
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4926..5055
                     /label=AAV2 ITR
     promoter        5211..5422
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5435..5703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5732..5741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5762..6424
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6494..6547
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6548..7147
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6548..6567)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7044..7064
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7201..7221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7263..7384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7300..7319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7354..7373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7535..7990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7622..7641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7832..7853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8007..8026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8126..8148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8186..8204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8272..8376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8377..8445,8446..9237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8595..8614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9408..9996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9897..9916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10058..10306
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10058..10078
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10229..10248
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10328..10403
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        10500..10785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(10787..10902,1..162)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ggggggcgcg cgccrggsgg gggggggggg gggggggggs ggggsggggs gaggcggaga
       61 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
      301 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
      361 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg gactataagg
      421 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
      481 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
      541 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      601 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      661 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      721 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      781 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      841 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      901 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
      961 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1021 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1081 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1141 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1201 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1261 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1321 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1381 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1441 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1501 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1561 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1621 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1681 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     1741 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     1801 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     1861 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     1921 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     1981 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2041 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2101 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2161 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2221 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2281 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2341 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2401 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2461 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2521 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2581 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2641 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2701 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     2761 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     2821 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     2881 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     2941 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3001 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     3061 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3121 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3181 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3241 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3301 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3361 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3421 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3481 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3541 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3601 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3661 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     3721 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     3781 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     3841 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     3901 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     3961 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4021 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4081 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4141 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4201 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4261 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4321 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4381 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4441 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4501 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4561 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4621 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag
     4681 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4741 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4801 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4861 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg
     4921 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4981 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     5041 cgagcgagcg cgcagctgcc tgcaggaaag agcccacaac ccctcacttg gtgggccagt
     5101 cctctgatag actgtgtccc ctggataccc gtattctaga gctagcgaat tcgaatttaa
     5161 atcggatccg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     5221 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     5281 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     5341 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     5401 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     5461 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     5521 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     5581 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     5641 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     5701 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct
     5761 gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct
     5821 ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac
     5881 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta
     5941 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg
     6001 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt
     6061 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt
     6121 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca
     6181 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg
     6241 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca
     6301 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca
     6361 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt
     6421 cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc
     6481 caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     6541 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6601 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6661 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6721 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6781 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6841 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6901 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6961 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7021 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7081 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7141 tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7201 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgcgttaac
     7261 taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7381 cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag ctcatctggt
     7441 ctcccttccg ggcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7501 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     7561 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     7621 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7681 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7741 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7801 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7861 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     7921 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7981 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8041 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8101 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     8161 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8221 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8281 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8341 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8401 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8461 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8521 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8581 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8641 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8701 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8761 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8821 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8881 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8941 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9001 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9061 attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg
     9121 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9181 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9241 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9541 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10021 cggttcctgg ccttttgctg gccttttgct cacatgtgag ggcctatttc ccatgattcc
    10081 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
    10141 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
    10201 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
    10261 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgaaa gccaggggtc
    10321 tggcggcgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
    10381 aaaaagtggc accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata
    10441 aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac aaatggctct agaggtaccc
    10501 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
    10561 acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10681 gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt
    10741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
    10801 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
    10861 attttttaat tattttgtgc agcgatgggg gcgggggggg gg
//
