LOCUS       sequence-337834-       10301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of T(Brachyury)-GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Aramaki S, Kagiwada S, Wu G, Obridge D, Adachi K, Kutejova E, 
            Lickert H, Hubner K, Scholer HR
  TITLE     Residual pluripotency is required for inductive germ cell 
            segregation.
  JOURNAL   EMBO Rep. 2021 Aug 4;22(8):e52553. doi: 10.15252/embr.202152553. 
            Epub 2021 Jun 22.
  PUBMED    34156139
REFERENCE   2  (bases 1 to 10301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-08-4"; volume: "22(8):e52553. doi"; pages: " 
            10.15252/embr.202152553. Epub 2021 Jun 22"
COMMENT     Sequence Label: pPB-CAG-T-V5-GR-2A-H2BmCherry-IP
FEATURES             Location/Qualifiers
     source          1..10301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        972..1355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1360..1637
                     /label=chicken beta-actin promoter
     intron          1638..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2578..2600
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2662..2681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4032..4073
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4938..4994
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4995..5372
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     regulatory      5385..5394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5391..6101
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5536..5554)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5804..5823
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6142..6716,6717..6719,6720..6728)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6309..6326)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6536..6555
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6729..7328
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6729..6748)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7225..7245
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7409..7426)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7415..7639
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7686..7702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7710..7726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7734..7740,7741..7758,7759..7764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7779..7800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8027..8089
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8113..8131)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8152..8168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8176..8192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8200..8206,8207..8224,8225..8230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8245..8266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8383..8400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8554..9142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8634..8653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9313..10104,10105..10173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9936..9955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10174..10278)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt
     1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct
     1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgccg gggggggggg
     1501 gggggggsgg ggggsggggs ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2221 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagaattcct cgaggccgcc gccatgagct cgccgggcac agagagcgca gggaagagcc
     2761 tgcagtaccg agtggaccac ctgctcagcg ccgtggagag cgagctgcag gcgggcagcg
     2821 agaagggaga ccccaccgaa cgcgaactgc gagtgggcct ggaggagagc gagctgtggc
     2881 tgcgcttcaa ggagctaact aacgagatga ttgtgaccaa gaacggcagg aggatgttcc
     2941 cggtgctgaa ggtaaatgtg tcaggcctgg accccaatgc catgtactct ttcttgctgg
     3001 acttcgtgac ggctgacaac caccgctgga aatatgtgaa cggggagtgg gtacctgggg
     3061 gcaaaccaga gcctcaggcg cccagctgcg tctacatcca cccagactcg cccaattttg
     3121 gggcccactg gatgaaggcg cctgtgtctt tcagcaaagt caaactcacc aacaagctca
     3181 atggaggggg acagatcatg ttaaactcct tgcataagta tgaacctcgg attcacatcg
     3241 tgagagttgg gggcccgcaa cgcatgatca ccagccactg ctttcccgag acccagttca
     3301 tagctgtgac tgcctaccag aatgaggaga ttacagccct taaaattaaa tacaacccat
     3361 ttgctaaagc cttccttgat gccaaagaaa gaaacgacca caaagatgta atggaggaac
     3421 cgggggactg ccagcagccg gggtattccc aatgggggtg gcttgttcct ggtgctggca
     3481 ccctctgccc gcctgccagc tcccaccctc agtttggagg ctcgctctct ctcccctcca
     3541 cacacggctg tgagaggtac ccagctctaa ggaaccaccg gtcatcgccc taccccagcc
     3601 cctatgctca tcggaacagc tctccaacct atgcggacaa ttcatctgct tgtctgtcca
     3661 tgctgcagtc ccatgataac tggtctagcc tcggagtgcc tggccacacc agcatgctgc
     3721 ctgtgagtca taacgccagc ccacctactg gctctagcca gtatcccagt ctctggtctg
     3781 tgagcaatgg taccatcacc ccaggctccc agacagctgg ggtgtccaac gggctgggag
     3841 ctcagttctt tcgaggctcc cctgcacatt acacaccact gacgcacacg gtctcagctg
     3901 ccacgtcctc gtcttctggt tctccgatgt atgaaggggc tgctacagtc acagacattt
     3961 ctgacagcca gtatgacacg gcccaaagcc tcctcatagc ctcgtggaca cctgtgtcac
     4021 ccccatccat gggcaagccc atccctaacc ctctgctggg cctggacagc accactagtg
     4081 cctctggtgg cggtggctcg ggcggaggtg ggtcgggtgg cggcggatca ctcatggaaa
     4141 atcctggtaa caaaacaata gttcctgcaa cgttaccaca actcacccct accctggtgt
     4201 cactgttgga ggttattgaa cctgaagtgt tatatgcagg atatgatagc tctgttccag
     4261 actcaacttg gaggatcatg actacgctca acatgttagg agggcggcaa gtgattgcag
     4321 cagtgaaatg ggcaaaggca ataccaggtt tcaggaactt acacctggat gaccaaatga
     4381 ccctactgca gtactcctgg atgtttctta tggcatttgc tctggggtgg agatcatata
     4441 gacaatcaag tgcaaacctg ctgtgttttg ctcctgatct gattattaat gagcagagaa
     4501 tgactctacc ctgcatgtac gaccaatgta aacacatgct gtatgtttcc tctgagttac
     4561 acaggcttca ggtatcttat gaagagtatc tctgtatgaa aaccttactg cttctctctt
     4621 cagttcctaa ggacggtctg aagagccaag agctatttga tgaaattaga atgacctaca
     4681 tcaaagagct aggaaaagcc attgtcaaga gggaaggaaa ctccagccag aactggcagc
     4741 ggttttatca actgacaaaa ctcttggatt ctatgcatga agtggttgaa aatctcctta
     4801 actattgctt ccaaacattt ttggataaga ccatgtccat cgagttcccc gagatgttag
     4861 ctgaaatcat caccaatcag ataccaaaat attcaaatgg aaatatcaaa aaacttctgt
     4921 ttcatcaaaa ggctagcgcc accaacttct ccctgctgaa gcaggccggc gacgtggagg
     4981 agaaccccgg ccccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     5041 agaaggcggt gactaaggcg cagaagaaag acggcaagaa gcgcaagcgc agccgcaagg
     5101 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     5161 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     5221 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     5281 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     5341 ctaaggccgt caccaagtac accagcgcta agcttgagac cagtgccacc atggtgagca
     5401 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5461 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5521 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5581 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5641 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5701 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5761 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5821 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5881 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     5941 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6001 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6061 gccgccactc caccggcggc atggacgagc tgtacaagtg agcggccgct cgataagctt
     6121 gatatcgaat tccgcccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     6181 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6241 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6301 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6361 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6421 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6481 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6541 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6601 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6661 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6721 ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6781 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6841 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6901 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6961 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7021 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7081 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7141 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7201 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7261 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7321 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     7381 catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7441 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7501 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7561 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7621 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa
     7681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatcctt
     7861 tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa
     7921 ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat
     7981 ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt
     8041 acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt taaagagctc
     8101 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     8161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8221 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10261 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
