LOCUS       sequence-337835-        9047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of T(Brachyury)-GR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Aramaki S, Kagiwada S, Wu G, Obridge D, Adachi K, Kutejova E, 
            Lickert H, Hubner K, Scholer HR
  TITLE     Residual pluripotency is required for inductive germ cell 
            segregation.
  JOURNAL   EMBO Rep. 2021 Aug 4;22(8):e52553. doi: 10.15252/embr.202152553. 
            Epub 2021 Jun 22.
  PUBMED    34156139
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-08-4"; volume: "22(8):e52553. doi"; pages: " 
            10.15252/embr.202152553. Epub 2021 Jun 22"
COMMENT     Sequence Label: pPB-hCMV1-T-V5-GR-IRES-H2BmCherry-pA
FEATURES             Location/Qualifiers
     source          1..9047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(1075..1093,1094..1116,1117..1135,1136..1158,1159..1177,
                     1178..1200,1201..1219,1220..1242,1243..1261,1262..1284,
                     1285..1303,1304..1326,1327..1345)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1378..1416
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1413..1437
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1539..2111
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             3489..3530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4408..4982,4983..4985,4986..4994)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4575..4592)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4802..4821
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4995..5372
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     regulatory      5385..5394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5391..6101
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5536..5554)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5804..5823
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6155..6172)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6161..6385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6432..6448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6456..6472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6480..6486,6487..6504,6505..6510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6525..6546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6773..6835
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6859..6877)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6898..6914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6922..6938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6946..6952,6953..6970,6971..6976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6991..7012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7129..7146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7300..7888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7380..7399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8059..8850,8851..8919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8682..8701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8920..9024)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gaggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
     1021 tgatctatag atctggatcc gtcgacaacg gcaagcttac atcgagttta ccactcccta
     1081 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
     1381 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1441 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1501 cgcgggactc agacacccgg gatcctgaga acttcagggt gagtttgggg acccttgatt
     1561 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     1621 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     1681 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     1741 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     1801 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     1861 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     1921 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     1981 ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     2041 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     2101 ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag
     2161 aattcctcga ggccgccgcc atgagctcgc cgggcacaga gagcgcaggg aagagcctgc
     2221 agtaccgagt ggaccacctg ctcagcgccg tggagagcga gctgcaggcg ggcagcgaga
     2281 agggagaccc caccgaacgc gaactgcgag tgggcctgga ggagagcgag ctgtggctgc
     2341 gcttcaagga gctaactaac gagatgattg tgaccaagaa cggcaggagg atgttcccgg
     2401 tgctgaaggt aaatgtgtca ggcctggacc ccaatgccat gtactctttc ttgctggact
     2461 tcgtgacggc tgacaaccac cgctggaaat atgtgaacgg ggagtgggta cctgggggca
     2521 aaccagagcc tcaggcgccc agctgcgtct acatccaccc agactcgccc aattttgggg
     2581 cccactggat gaaggcgcct gtgtctttca gcaaagtcaa actcaccaac aagctcaatg
     2641 gagggggaca gatcatgtta aactccttgc ataagtatga acctcggatt cacatcgtga
     2701 gagttggggg cccgcaacgc atgatcacca gccactgctt tcccgagacc cagttcatag
     2761 ctgtgactgc ctaccagaat gaggagatta cagcccttaa aattaaatac aacccatttg
     2821 ctaaagcctt ccttgatgcc aaagaaagaa acgaccacaa agatgtaatg gaggaaccgg
     2881 gggactgcca gcagccgggg tattcccaat gggggtggct tgttcctggt gctggcaccc
     2941 tctgcccgcc tgccagctcc caccctcagt ttggaggctc gctctctctc ccctccacac
     3001 acggctgtga gaggtaccca gctctaagga accaccggtc atcgccctac cccagcccct
     3061 atgctcatcg gaacagctct ccaacctatg cggacaattc atctgcttgt ctgtccatgc
     3121 tgcagtccca tgataactgg tctagcctcg gagtgcctgg ccacaccagc atgctgcctg
     3181 tgagtcataa cgccagccca cctactggct ctagccagta tcccagtctc tggtctgtga
     3241 gcaatggtac catcacccca ggctcccaga cagctggggt gtccaacggg ctgggagctc
     3301 agttctttcg aggctcccct gcacattaca caccactgac gcacacggtc tcagctgcca
     3361 cgtcctcgtc ttctggttct ccgatgtatg aaggggctgc tacagtcaca gacatttctg
     3421 acagccagta tgacacggcc caaagcctcc tcatagcctc gtggacacct gtgtcacccc
     3481 catccatggg caagcccatc cctaaccctc tgctgggcct ggacagcacc actagtgcct
     3541 ctggtggcgg tggctcgggc ggaggtgggt cgggtggcgg cggatcactc atggaaaatc
     3601 ctggtaacaa aacaatagtt cctgcaacgt taccacaact cacccctacc ctggtgtcac
     3661 tgttggaggt tattgaacct gaagtgttat atgcaggata tgatagctct gttccagact
     3721 caacttggag gatcatgact acgctcaaca tgttaggagg gcggcaagtg attgcagcag
     3781 tgaaatgggc aaaggcaata ccaggtttca ggaacttaca cctggatgac caaatgaccc
     3841 tactgcagta ctcctggatg tttcttatgg catttgctct ggggtggaga tcatatagac
     3901 aatcaagtgc aaacctgctg tgttttgctc ctgatctgat tattaatgag cagagaatga
     3961 ctctaccctg catgtacgac caatgtaaac acatgctgta tgtttcctct gagttacaca
     4021 ggcttcaggt atcttatgaa gagtatctct gtatgaaaac cttactgctt ctctcttcag
     4081 ttcctaagga cggtctgaag agccaagagc tatttgatga aattagaatg acctacatca
     4141 aagagctagg aaaagccatt gtcaagaggg aaggaaactc cagccagaac tggcagcggt
     4201 tttatcaact gacaaaactc ttggattcta tgcatgaagt ggttgaaaat ctccttaact
     4261 attgcttcca aacatttttg gataagacca tgtccatcga gttccccgag atgttagctg
     4321 aaatcatcac caatcagata ccaaaatatt caaatggaaa tatcaaaaaa cttctgtttc
     4381 atcaaaagtg agcggccgcg aattccgccc ctctccctcc ccccccccta acgttactgg
     4441 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4501 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     4561 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4621 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4681 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4741 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4801 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4861 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4921 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     4981 atatggccac aaccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     5041 agaaggcggt gactaaggcg cagaagaaag acggcaagaa gcgcaagcgc agccgcaagg
     5101 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     5161 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     5221 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     5281 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     5341 ctaaggccgt caccaagtac accagcgcta agcttgagac cagtgccacc atggtgagca
     5401 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5461 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5521 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5581 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5641 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5701 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5761 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5821 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5881 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     5941 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6001 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6061 gccgccactc caccggcggc atggacgagc tgtacaagtg aactagcttg gccgctcgat
     6121 aagcttgatc gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     6181 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6241 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6301 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6361 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccaagcttg
     6421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     6601 atcctttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     6661 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     6721 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     6781 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa
     6841 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     6901 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6961 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7021 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7081 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7741 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8341 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8641 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8821 atcttttact ttcaccagcg tttctgggtg agcaaaaacg ggaaggcaaa atgccgcaaa
     8881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
