LOCUS       sequence-337837-        7907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression a mutated 3'UTR of the human ARID3A gene
            after a GFP, to evaluate miRNA binding. Regions not containing 
            miR-125b or let-7c binding regions not included..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     437..458
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(526..737)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    807..924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1137..1181)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1366..1599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2092..2217
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2264..2444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2445..2671)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     enhancer        2690..2881
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2838..2858
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     enhancer        2938..3317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3362..3382)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3362..3380)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3401..3417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3401..3417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3414..3436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3425..3441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3449..3455,3456..3473,3474..3479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3494..3515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3632..3649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3803..4391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3883..3902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4562..5353,5354..5422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5185..5204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5423..5527)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5553..6008)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5690..5711)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5902..5921
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6135..6157
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6149..6166
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6150..6166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6176..6195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6176..6194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6215..6350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6390..6511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6401..6420)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6455..6474
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6589..6822
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    6894..7482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7000..7011
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7409..7429
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(7691..7907,1..497,498..500,501..503))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7694..7907,1..497,498..500,501..503))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7736..7757)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
       61 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      181 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
      301 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      361 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      481 agctcctcgc ccttgctcac catggtggcg gatccgtcac gacacctgtg ttctggcggc
      541 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
      601 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
      661 ccttctctag gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg
      721 cgatgtgcgc tctgcccact gacgggcacc ggagccaact cgagccggcg ccaaagtgga
      781 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
      841 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
      901 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct caagatctag
      961 aattgtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     1021 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     1081 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     1141 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     1201 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     1261 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     1321 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     1381 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     1441 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     1501 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     1561 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     1621 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     1681 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     1741 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     1801 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     1861 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     1921 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     1981 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     2041 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     2101 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     2161 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     2221 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     2281 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     2341 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     2401 ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct
     2461 aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc
     2521 agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg
     2581 catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg
     2641 caagactaca agagtattgc ataagactac attaaggcgc gcctcttaag ggactatggt
     2701 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     2761 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     2821 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     2881 gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccctgcat ggcgcgccat
     2941 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     3001 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     3061 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     3121 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     3181 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     3241 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     3301 agtcaataat caatgtcaac ctgcaggtcg acggtaccgc ttgcagctcc agcttttgtt
     3361 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     3421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5521 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     5581 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     5641 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     5701 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     5761 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     5821 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5881 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5941 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     6001 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6061 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6121 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     6181 cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac
     6241 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     6301 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     6361 aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac
     6421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     6481 tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg
     6541 caagttataa atactgaata ataagatgac atgaactact atacgtactg ctagagattt
     6601 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     6661 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     6721 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa
     6781 aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta
     6841 aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagtc gacgcgggga
     6901 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     6961 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     7021 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     7081 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     7141 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     7201 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     7261 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     7321 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     7381 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     7441 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgactt aacgcgggct
     7501 gcgcttagtc agcgacagag agtagtaaag acctcagaca gacagagtcc acggcaccta
     7561 gatccgagaa gaacctagac ttcgaggcat cctcacacct agtgaggtcc acattcatca
     7621 cctagacaag atgcctgggt gctttttcaa gaccagtttc acctaaggaa cacgatgtat
     7681 cagacaaaca ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     7741 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
     7801 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     7861 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcgg
//
