LOCUS       sequence-337853-       19005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length S. pombe Mdn1 in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19005)
  AUTHORS   Mickolajczyk KJ, Olinares PDB, Niu Y, Chen N, Warrington SE, Sasaki 
            Y, Walz T, Chait BT, Kapoor TM
  TITLE     Long-range intramolecular allostery and regulation in the 
            dynein-like AAA protein Mdn1.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. doi: 
            10.1073/pnas.2002792117. Epub 2020 Jul 21.
  PUBMED    32694211
REFERENCE   2  (bases 1 to 19005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2002792117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-08-4- 4"; volume: "117"; issue: "31"; pages: 
            "18459-18469"
COMMENT     Sequence Label: Mdn1-FL
FEATURES             Location/Qualifiers
     source          1..19005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      663..1251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1152..1171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1405..1422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  1556..1780
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1847..2380)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2569..2574,2575..2591,2592..2597))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2949..3040
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2965..2982
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3107..3124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3146..3166
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    17540..17674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17577..17596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     17631..17650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  17703..17868
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      18052..18506
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18139..18158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     18348..18369
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        18532..18636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(18637..18705,18706..19005,1..492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(18855..18874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
       61 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      121 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      181 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      241 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      301 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      361 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      421 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
      481 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
      541 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
      601 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      781 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     1381 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     1441 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     1501 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     1561 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     1621 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     1681 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     1741 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     1801 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     1861 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     1921 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     1981 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     2041 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     2101 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     2161 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     2221 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     2281 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     2341 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     2401 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     2461 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     2521 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     2581 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     2641 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     2701 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     2761 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     2821 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     2881 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat
     2941 attaatagat catggagata attaaaatga taaccatctc gcaaataaat aagtatttta
     3001 ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt attcataccg
     3061 tcccaccatc gggcgcggat ctcggtccga aaccatgtcg tactaccatc accatcacca
     3121 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tggggatccg
     3181 gaattcaaag gcctacgtcg acatggatgt gttgattgag tgggttgcaa tataccctca
     3241 gatatatgat attttggagc atattaatta cgttccaagc aacactttgc aacgtttgcg
     3301 tttgcaccaa ccctggtcaa aaattgacta cgacgtttgg tttttgtatg catcggacga
     3361 aattcgtgaa acctgcaaag tcaaatatta tggagaaaca aagacttacg gtgaagtttt
     3421 tgttttagaa aatgaaagaa tatcccaact tcatcgtctt tttgtttctt ggacggtctc
     3481 tgaaagagca gaacatctaa agaatttact atttgacgct ggattatcca atttacctct
     3541 tgttgaacta gggggaaatg tcttttttaa ctctcacgtg ccgcttcctt gttccctagt
     3601 tttaactaaa agtacacagg aaaatttaaa tcgcataact ccttacttgg tacaaaaaag
     3661 accaattctt ctggctggtc cagaaggaat tggtaaaaag tttttaatca cacaaattgc
     3721 tgcgaaattg ggacaacaga tcattcgtat tcatcttagc gattccacag accccaaaat
     3781 gttgattgga acatatacct ctccaaaacc tggagaattc gagtggcaac ctggtgtgtt
     3841 gacacaagct gtaattactg gaaaatggat cttgtttaca aatatcgagc acgcaccttc
     3901 tgaagtgctt agcgtcttgc ttcctcttct cgaaaaaagg caactcgtaa ttccctcacg
     3961 tggtgaaaca atatatgcta aaggatcctt tcaaatgttt gctacatcat ctatgaaaac
     4021 aaagattctt gggcaacgat tatggcagat acttgatttg acttatcaac cagatgaatg
     4081 tgttgaggta gtatctactc tatatcctgt tttatcaatc atttgtccta ctttgtactc
     4141 tgtttacaaa gatatatttg atcttttttc acaaagatcg ttccttgcca cctcaaaaat
     4201 atatcgtcga ttgtgtttgc gtgatttcta caaatttatt aaaagggttg cgtttctata
     4261 tcacaaattt atgatccctt ccgaccatgt agtcatatct caagaactcc aagatgcagt
     4321 ttttaaagag gctatcgata tgtttggtgc gtttattcct tctagagacg gttttgatct
     4381 tgtggtgcgt aacgtcgcta tagagttaaa tataccacct gaaaaggctt tacaattacg
     4441 atattctatt cctgtatttc aaaatttgga acataacata aacataggta gatgttcact
     4501 taagaaattg tctactattc gttcttgttc cactaattca tatgctttca catcttcatc
     4561 tcttggctta ttggaacagc tggctgcagg tgttcaaaca aacgaaccat tgcttctagt
     4621 tggtgagact ggtactggta agaccactac tatccaactt ttagctggct tgttaggtca
     4681 gaaagttact gtaattaata tgtctcaaca gacggaatct tctgatatgc ttggcggtta
     4741 taaacctatc aacgcttcca cacttggtct tccattgcat gagaggttta tagatatatt
     4801 tgagcaaact tttagttcca agaaaaatgc taagtttata tccatggcat caacgtcagc
     4861 acgacggttt cgttggaaaa cttgcctaaa aatttggaaa gaagcttgca aattgtctaa
     4921 aacggttctt gatggtcaac aacctcttcc taatcctcaa aaaagacaaa aacgtttatc
     4981 taatcaggtt gagcttcgaa atcagtgggc taaatttgag aaagaggttg aagattttga
     5041 aaaagttctt acgggaggtt ccaatggctt catgttttct ttcgttgagg gtgctttggt
     5101 gaaggcggtt cgttccggac actgggtatt actagacgag attaatttgg catcattaga
     5161 gactttagaa cctattggcc aacttttaag ctcatatgaa tcgggtatac ttctcagtga
     5221 acgtggcgat ataactccaa ttactcctca taaaaatttt aggttatttg gctgtatgaa
     5281 tccttctaca gatgttggaa aacgtgaact tgagccatct tttcgatcaa gatttactga
     5341 aatatatgtt cattctcccg atcaaaattt agatgattta ttatcaatca ttcaaaagta
     5401 tatcggttct ctttgtattg gtaatgaaca tgttatccga gaggtcgctg aactatatca
     5461 agttgcaaag agtctttctc tcgatggttc tcttgttgat ggagctggtc aaagaccaca
     5521 ctataccgtt cgaacattga gccgaacttt gtcgtatgta acggaaattg ctccaattta
     5581 tggattgaga agatcccttt atgagggatt ttgtatgtct tttttgacgt tactcgacca
     5641 tactagtgaa agcttattgt acaaccatgt ggttcgtttc acactaggtg agctaaatag
     5701 ggatcaacag aatgcaatac tcaagcaaat ccctaaagta cccgatcatt ctagttatat
     5761 tgctttttgc cattactggt tgagacgtgg gtctttcccc gtagaggaac aagagcatta
     5821 tatcataaca ccatttgtgc aaaaaaattt attaaacata gctagagctt gttctactag
     5881 gatgtttcca attttaatcc aaggaccaac ttctagtggc aaaacaagta tgattgaata
     5941 tgtagctaaa aagactggac ataagtttgt tcgaataaac aatcacgaac acactgatct
     6001 acaggaatat attggcacct acgttactga tgataacggg tcattgagct ttagggaagg
     6061 cgttttggtg gaagctttac gaaatggata ttggattgta ttagacgaac tcaatctggc
     6121 ccctaccgac gttttagaag ctttgaatag acttttagat gataatcgtg agctttttat
     6181 acccgaaaca caagttttgg ttaaaccaca tcctgagttt atgctttttg caacacagaa
     6241 tcctcctggt gtttatgctg gtcgtaaaca tctttctcga gcattccgca atagattctt
     6301 agaaattcat tttgatgaca ttcctgaaaa tgaactggag acgattcttc ataagcggtg
     6361 taaaatagcc ccttcatatg ctgcaaagat tgtacaagta ttccgcgagc tatctcttcg
     6421 aagacaaacc acccgaattt ttgagcaaaa aaattctttt gctactttga gagatttatt
     6481 tagatgggcg tttcgcgaag ctgtcggcta ccaacagtta gcagaaaatg gttatatgct
     6541 tttggctgaa cgcgcgagag accaaaagga caaactggcc gtgcaagaag taatcgaaaa
     6601 agttatgaag gtaaaaattg acactgatgg tatttataac cttgactcta tggagatctt
     6661 ccaggatatg agtttaaaag agggtccttt atctaaggtt gtttggacac gtccaatgat
     6721 tcgtttattt tgcctcgtat ggcgctgttt actagcaaaa gaacccgttt tgttagttgg
     6781 agataccggt tgtggtaaaa caactgtttg tcagattcta gctgagtgtt tgcacaaaga
     6841 gttgcatatt attaatgctc atcaagatac ggaaaatgga gacataattg gtgctcaaag
     6901 gcctgtaagg aatcgttccg ctgtaaatta cagtcttcat tcacaattat gtgaaaaatt
     6961 taacgtacaa gaaagtctgg attctattga cgatctcatt gagaaatttg aaaaactttc
     7021 ttcttctgaa aagaacgaca atttatctaa tctgatcgaa cgccagatta tcaagtaccg
     7081 ctctctgttt gaatggcatg acggtgcttt ggttaccgcg atgaagcaag gcgacttttt
     7141 tttacttgat gagattagtc tcgccgatga ttccgttttg gagagattga atagtgttct
     7201 tgaattatct cgtactctta ctttagtgga acacagtaat gctgccgtat ctcttactgc
     7261 caaagatgga tttgcatttt ttgctactat gaatcctggt ggtgattacg gtaagaagga
     7321 actttcgcca gcgttgcgga atcggtttac cgaaatttgg gttccaccga tggtcgatac
     7381 agaggacatt cttaaaattg tcgaaggcaa acttcataac aacaaaatcg agttagctcg
     7441 ccctttggtt gaatatgcga aatggcatgc gaatgaatac ttgtatactg atgtgatttc
     7501 tattcgtgat gtcttatccg cagtagaatt tataaatgca tgcgaaattt tggatttaaa
     7561 tctggtattg tttaatgctg tttcaatggt ttttatagat gcgcttggtt ctttcacgac
     7621 cttttcttta agtaacaatt tggcatcctt acacgctgaa cgtcaaagat gtttcgctaa
     7681 attaaatgag ttagcaggat ctaacattat ggcatccaaa tctgccgata tatcaattaa
     7741 attctccgac tcatcatttt ttataggtga ttttggaata cctttaggag attctgttga
     7801 atcggattcg acttatagtt tacatacaga tactacttta atgaacgctt ccaaggtttt
     7861 gagggctctg caggttctga aacctatttt actggaaggt agtcctggag ttggcaaaac
     7921 cagtcttatt acagcacttg ctcgtgaaac aggacatcaa ctggtgcgta taaatttgtc
     7981 tgatcaaaca gatttgatgg atcttttcgg ctcagacgtt cctgtggaag gtggtgaagg
     8041 agggcagttt gcttggcgtg atgctccttt tttggcagcg atgcgtaatg gtcattgggt
     8101 gttgcttgat gaattaaatt tagcttcaca gtcagtcttg gaaggcttaa atgcttgtct
     8161 tgatcatcgt aatgaagctt atataccgga attagacaag gtgtttaaag ctcatccgaa
     8221 ttttcgagtt ttcgctgccc aaaatccaca gcatcagggt ggcggtagga aaggcttacc
     8281 gaggtcgttc atcaatagat tttcggttgt ttacgttgag gctcttaaag aaaaggacat
     8341 gattgaaatt gcagcatgta actatcatca agtaaatgag gattggcgtt tgaagattat
     8401 caagtttatg tttcgtttgc aagacaatat tgagaaagac attagttttg gtagttttgg
     8461 aagcccttgg gaatttaatc ttcgtgatac tttacgttgg ttacaacttt tgaatgatgc
     8521 tccaaaatat acatgtgttt cacctgctga ttatttggaa gtcatggtcc tgcatcgtat
     8581 gagaacggtt gaagaccgtg ttagaacatg cgaattgttt aaagaagtat ttgatataga
     8641 ttatgagccc cgtactattg gttttagctt gagcagccaa tgttttaaag tcggacattc
     8701 acttcttgtc agggatgtcg aaagacagaa aactttattg gacagtcaaa atatacttca
     8761 atcacaatta ccagttctcg aatcggtaat aacatgcatc aacaaaaaat ggccctgtat
     8821 tttggtgggc gacactgcca cagggaaaac gtgcattctc cgtttgcttg cggctattgc
     8881 tggtgctaaa attaaagaaa tggctgttaa ttccgacact gatacaatgg accttattgg
     8941 tgagtatgag cagatagata tatctcgaaa agcatcagag ctttttacgg atctttctca
     9001 acaattatta aatattgtca tcaaatatag gaatttcgat aatattttcc gtgaaacttc
     9061 cttatataca ttaacgacga cttctttcaa aacacactcc caagcgttta cactgcttca
     9121 aaaagtagtg gaccaattgg atcagttaaa gatacatgaa accctagtac attctttagg
     9181 tgatattcat gaaaaggctc gtaaactgct tgcagagttt tcagcttctc cagcaggtcg
     9241 gtttgaatgg tttgatggct atttactaaa ggctgttgaa gaagggcatt ggtttgtctt
     9301 ggataacgct aatttatgta gtcctgctgt ccttgatcgc ctaaactcat tgctagaaca
     9361 taaaggagtc ttaattgtca atgagaaaac tactgaagat ggacatccca aaaccataaa
     9421 gccgcatccc aactttcggt tgtttttaac cgtaaatcct gtttacggtg aactatcacg
     9481 agcaatgagg aatagggggg tggaaatatt cttgttaaaa gaagccctta ccgagattga
     9541 caagaagcaa atgtctcttc tagaacctgc accgatatca tctgctgtgg atacgcttgc
     9601 ttcaaatatt tcgtacatca aatatgtttt tgaaaccatg ggtaaaattg aaatagatgg
     9661 taattatatg tacattgccc atgctataat ccttgcgtta ttttctccaa ggcaactaaa
     9721 acttcttcga aaagttttat taactaatcc tcaattttcg ttatctatca aagctgatgc
     9781 agaacttttg cttaccttaa aaaatttggt acaaaaaata tattgcgctg attattttaa
     9841 tcatatggat ttaaaggctt cgaggttcat ggatatatat gaatatcccg tccagcttcg
     9901 tgaagttgta ggcctgatac aaacgattaa tgattttcaa tcagtcatat taacctccca
     9961 tttggagttg cctgagacat atgcatcagg tttattgttt gtttctgcac atgaaattct
    10021 cgacttaaca gaggaagtca accgcttagc agtttccaca agcaatagta cctaccttct
    10081 caaaagtgcg tctgctgttt atcataacgt ttcttctttt aaagggtcta ctccttcttt
    10141 gtggaactta ttaaatcagt tttctaaatt tttgattgaa attgcaagtg caaactcaaa
    10201 cattgtctat aagctatcct acgacgttat tcgtcatttt ctcaaactag ttgtattgtg
    10261 gaaaaatata tacgtttgga caaatgttcc cgattgtgat attagtagat tttattgtta
    10321 cactaaaatg ttaggagaat ggatgtttac tttaacggaa aaaacaaagc tattggagtc
    10381 atttttacct aaggattcgc ttgaaaaatt ttcagaattg caaaacttgt ctactgggtt
    10441 acacatgcaa gcaatctggg acaaatggca tgctttcgtt cctcgtacat atgatcaatg
    10501 gtcgctttgg aatacagttg ataaactatt gacacaatat gttaatgcca acatcccatc
    10561 aatttctatg gaaactacgg catgtgaagt tgtaggaact tctttatcac tattaaacaa
    10621 agttctggtt gaaaacgaag ttggagacat ttattcatat ctcaaaatat tgggtaaagg
    10681 tgttaatgag ctaaaatctt ctaaacaagt tattttaccc gaaaaccttg ttaatctttt
    10741 taactgttta gcttcattgg atttgttgca tatttttatc aaatatacta cctcctcgtt
    10801 ctttctaacc gacgattttg tgcgttttat acgagtttgc tttcattcca gaatttcagg
    10861 taatctgctg acgttactgc atggaattag tttcgatagc acgaaagctg tagcaccagt
    10921 tttaacctac ttcgattttt gctctttaac caccggcaac atcttaggtc gtattgcact
    10981 tgcttttacc agcattgatg agaatgcaaa tttggagtct gcaaatattt ttgagcatgc
    11041 acgattggcc ttacttcagc atttcatgga tcactcttct ttattagcgg aggactcatc
    11101 cacaaaaatg aatttgatat tgctacagag atatgctgtt ataatttcaa tattcttgga
    11161 tcaaggaaag tgtgaaaagg ccaatgacct tataacgaag ctctctcttc cttatgagga
    11221 attagctgaa aactttgtct ctatattaga agcttgcaaa gcatttttgg ttgccaattc
    11281 agaattcatt tcttacacgt atactgagcg ttttattcat tctttgagat ttctgaaaga
    11341 ctcttggtta agcagcaacc aacagaaaat gttgaaaaat caaggaatgg cttatattta
    11401 ttttgcttct ggaatgctgt tagtatatgt tccagacaaa ccctttgatc ctgccctgct
    11461 tcctttgctc acagtagaaa gtttgagaca ttaccttgaa agtctctata aggaaagcca
    11521 aattttagaa attgccgagt ccttgaattc aggaaaagtt aatagcgtga tgagacgttt
    11581 agtttcgact gaaatttcga acacgcctaa cattgattct tctttcagta ccgtgtacag
    11641 atcactgaat gagagcatag ttcctcttta tagtgaattg gaatttttca tgaagtctgt
    11701 tgtacttaat cagtacatat ttgagctagc aatgcggctt tctaaagaat caaatatcgc
    11761 tgttgttgaa gaagctaaat cttttgtaac aaaatggaag gcctatattg aaagaatacg
    11821 tgaggcttat cctcaatttg ttgatgttta cgagctcatt ctttcattca taagtttcat
    11881 gatatacgga atagagctgc ttatgttcga agctaaacga cgcttggatg aacgatctca
    11941 gatactctca acgcttattt tgacattagt ggatccttca agttttgcac gttcgcttag
    12001 ctttgatgat gtttcaaatt taattgagca gatcaaagtg cttgatttaa atgattcaat
    12061 cagatttgaa atttacttat tcttagcttc tcgcttatgt agtgaaaaac agcattctag
    12121 tgatactcat tcgttggcca attctttcgt actattagct aatgaatttt acatccataa
    12181 tgcaaaaatt aagcagaaag agctcgagga gatagaagaa aaaaacagac tttatcgaca
    12241 gagagaattc aattttgata agaatgatta cttaaaagtg ttcattaatt acgacgatga
    12301 agttgaacct gaagttgaac ctgaagtcgt aattgaaaga aaaagattct tacaactgca
    12361 attcgctttt tggtctctct ataacgaaat atattctgag aaaatgaacg taataccgtt
    12421 ggagcagctt atgaataccg ggtcatatct tgctaagaaa attaaagtta agaatccaga
    12481 catgattgct tcatcaggtt ttgatattgt tagcgttgta ctcatgatgg gtgttaaatc
    12541 gactaacgaa agacagtatt ggaccccacc tgtttacaat ttttattctg atccgaaccc
    12601 ttcaaaagct atagaagttc gcgatttgat caaaattgtt gaaagccgag ctatatcttt
    12661 aatcaaaaac tggcctgaga attttgttct aagaggctta aaagatgcca ttgatgctat
    12721 acttaactta agtccgtttt ctcctattgc tgaatatcta tcaaaacttg aacgagtgtt
    12781 tcatttacta tctgaatggg aaaagttggc tagtagagaa tattcgcttg ctaatgaaat
    12841 ggacttgatt aagaaaaaaa tcattgattg gagaaagttt gagctttcga attggaacaa
    12901 tttgcttaaa ttagaagaat acaaactttc tgagagggtt tatccaagat tgtatagtat
    12961 cctgcaattt attatactga agcctttctt tgagaatagc aaattcacta agcaaaattt
    13021 gtgcgaatct gcctctatta tagttcagtt tatcaccgat ttgactgttg gggagtttca
    13081 actttgtttg aaatgcttgt tatcattttc tcaacatgct gcttctttaa gaatatgcca
    13141 cgggattgat gcaatgctcc taaatatcta tcattacttt gagcaatttt tatccaaagt
    13201 ttctgaggca attcatacac aaaaacagtc gcttgaaaat tcaatcaaag agcgaatact
    13261 attgatgagt tggaaggata ccaatgttta tgcgttgaag gaaagtgcta aaaagagtca
    13321 tgctgagtta tttaaagttc tacataggta tagagaagtt ttgagacagc ctgtttcttc
    13381 atatctttcg caaaaacatg attgggattc cttacttgac actgaaaaca attcagcaat
    13441 gtgggttgcc aaaaaagtaa accttagtcc ctcatatatt gaaaaaatgg atacagaaat
    13501 tatgaagctg gttccagtac ggttttctaa tactccgact actctaaggt tgatgtggac
    13561 tttatttgct aacgttgaaa agcctggttc aactttcact aatatggttt caaatttaat
    13621 tactgatgcc cgtgagctga tgaagctcac accggaaacc attaacgatg ataatctgtc
    13681 tgaaattaag catttgaaaa gtcgaaagca tttacttctt actgaaacat tcaaaactct
    13741 aaaggctttt ggcttgcaat accgtgttaa agctggtatt gaggaaaatc ttagtaattt
    13801 acgaaatttg ctggcagtta ttcctacttt tccagttact tcattgagta tagaaaaagt
    13861 tgatcgttca ctgatgaaat cattagactt tattcccaaa tttcagacac tagctggtca
    13921 tcaacataat gatttaagtg ttcctgaagt tcaaaaaggt gtaggtttat ttaacagtat
    13981 gttatctttg cagttaggtg agcgtgcaca gctagtcgaa tttaccaatg aattattggc
    14041 cttaaaaaat gtttattcag aagttggtgt gaatggttcg ccgttggaat cctttaataa
    14101 ttcttcattt aatgaggtga gtagtctggg atatgatcac gatttcgaaa atcgagcgca
    14161 ggctgtctct atgctttgtc aaatctatgc catagttata cagaagcact caagtatatc
    14221 tcccactgct tcatttcaat cgatcgggca tgaacttagt cgattcgccg acctgctatc
    14281 taacaagttg tttccttcct caattccgct ttatgcatct gctgataaag tttcttcgat
    14341 tcgtgatcaa cagaaaggaa taaatgattt gattgaatac tgtcgcaaaa aaaggacaga
    14401 attgccggag ttgtcttact gctttaaaca tttggttagc ttgcaaagtt tgaagtcaat
    14461 atccagaacg caagtggatt taacaaatga cgagtttttg aatttgatga actttgtttt
    14521 aaacttattt gatagtttgc tttcttcaat agaaaccgct acaaagaata tgcgaacatt
    14581 caaagaacta gccgaaacgt ctagttttat cgaaatgagc agttgctttt ctaaagtttt
    14641 gcgagctttt aatttaaagt ttcagtctat gaagctgagt tctttgaaag agaagttacg
    14701 ttcttcttct gtcgataaaa tgagttgtca actacttatg ctttttcttc ctgtttgtga
    14761 gcagtttata aacttagcgg aaagtgtgct agattacttt ataaatgttc ataactcaaa
    14821 ccttgattcg ctatccaaaa tttcgacgtt gtttttcatg gttgcaaaca atggtttctg
    14881 ctcgcctgac cttccacaag aaggaaaatc aaatagtggc gaattagagt ctggtactgg
    14941 tctcggttcc ggtgtgggag ctgaagatat cactaacacg ttaaatgaag acgatgacct
    15001 tgaagaactt gccaacgaag aagatacggc taatcaaagc gatttggacg agtctgaagc
    15061 tcgagagttg gaatcagata tgaatggagt aacaaaggac tctgtagtat cggagaacga
    15121 aaacagtgat agcgaagaag agaatcaaga tttggatgag gaagtcaatg atatccctga
    15181 agatttgtct aattcactga acgaaaaact ctgggacgag cccaatgaag aagatttgct
    15241 tgaaactgag cagaagtcaa atgaacagtc ggctgcaaac aatgagagcg acttggtttc
    15301 aaaggaagat gacaataaag cattagaaga taaagatagg caagagaaag aggacgaaga
    15361 agaaatgtca gatgatgttg gtattgatga tgaaatacaa cctgatattc aagaaaataa
    15421 tagtcaacca ccacctgaaa atgaggatca tctcgactta cctgaggacc tcaaactaga
    15481 cgaaaaagaa ggtgatgtta gcaaagattc agacttggaa gatatggata tggaagcagc
    15541 tgatgaaaac aaagaagagg cagatgctga aaaagatgaa cctatgcagg attttgaaga
    15601 tcctttggaa gagaacaaca ctctggatga ggatatccag caagatgatt tttctgatct
    15661 ggctgaggat gatgagaaga tgaatgaaga tggcttcgaa gagaatgttc aggaaaacga
    15721 agagtctact gaagatggag taaaaagtga tgaagaatta gagcaaggtg aagttcccga
    15781 agaccaggcg atagacaatc atcctaaaat ggatgcgaaa tctacttttg catctgcaga
    15841 agctgatgaa gaaaatacag ataaaggcat tgttggagaa aatgaagaat tgggtgaaga
    15901 agatggagca gcagaatctg gagttcgagg taatggaact gctgacggag aattttcatc
    15961 agcagagcaa gttcagaaag gggaagacac ttcaacccct aaagaagcaa tgagtgaggc
    16021 ggatcgacaa tatcaaagcc ttggtgatca tcttagagaa tggcaacagg ccaatcgtat
    16081 tcacgaatgg gaggatctta ctgaatcgca aagtcaagca tttgacgatt ctgaatttat
    16141 gcatgttaaa gaggatgagg aagaggattt gcaagctttg ggaaatgccg aaaaagatca
    16201 aattaagagt attgatcgag atgagtctgc taatcagaat ccagactcaa tgaacagtac
    16261 aaacattgct gaagatgaag ctgatgaagt tggagacaaa caattacaag atggtcagga
    16321 tatttcagat atcaagcaga ctggtgaaga tacccttcca acggaatttg ggtctatcaa
    16381 ccagtctgag aaggtttttg aattatcgga ggatgaggat attgaagatg aacttcctga
    16441 ttataatgtg aagattacta accttcctgc tgcaatgccc attgatgaag ctcgtgattt
    16501 atggaataaa cacgaggact ctacaaagca actttctatt gagttatgtg aacagcttcg
    16561 attgatatta gaaccaacct tggccactaa gatgcaagga gactttcgta cgggtaaacg
    16621 gttgaatatg aaacgaatca tcccatatat tgcttctcag ttcaagaaag acaagatatg
    16681 gatgcgtcgt gttaaaccta gtaaaaggac gtatcaagtg atgatttcca ttgatgattc
    16741 taagtcgatg agtgagtcgg gcagcacagt tctagcgtta gagactttag ctttggtaac
    16801 aaaagcatta tcattacttg aagttggaca aattgcagtg atgaaatttg gggagcaacc
    16861 tgaattgttg catcctttcg acaaacagtt ttcatctgaa agtggtgtac aaatgttttc
    16921 gcattttaca tttgagcaaa gtaataccaa tgttttggct ctcgccgatg cttctatgaa
    16981 atgctttaac tatgccaata cggcttccca tcatagatct aactcggaca taaggcagtt
    17041 agaaatcata atttcagacg gtatttgtga agatcatgat agcattcgga aactgctaag
    17101 gcgtgctcaa gaagaaaaag tgatgattgt gtttgttatt cttgacaatg ttaacaccca
    17161 gaaaaagtcg tccatccttg atattaaaaa ggtttactac gacactaaag aagatggtac
    17221 tatggattta aaaattcagc catatataga tgagtttgct tttgattact atttggtggt
    17281 tcgtaacatc gaagaattgc cgcaactttt gtcttctgca cttcgtcaat ggtttcagca
    17341 gatgagtaat acttaacccg gggcggccgc tttcgaatct agagcctgca gtctcgaggc
    17401 atgcggtacc aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
    17461 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    17521 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    17581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    17641 caaactcatc aatgtatctt atcatgtctg gatctgatca ctagatctgc ctaggagatc
    17701 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
    17761 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
    17821 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
    17881 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
    17941 cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt
    18001 ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct
    18061 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    18121 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    18181 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    18241 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    18301 gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt
    18361 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    18421 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
    18481 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
    18541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    18601 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    18661 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    18721 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    18781 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    18841 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    18901 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    18961 aagcatctta cggatggcat gacagtaaga gaattatgca gtgct
//
