LOCUS       sequence-337855-       16587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Mickolajczyk KJ, Olinares PDB, Niu Y, Chen N, Warrington SE, Sasaki 
            Y, Walz T, Chait BT, Kapoor TM
  TITLE     Long-range intramolecular allostery and regulation in the 
            dynein-like AAA protein Mdn1.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18459-18469. doi: 
            10.1073/pnas.2002792117. Epub 2020 Jul 21.
  PUBMED    32694211
REFERENCE   2  (bases 1 to 16587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2002792117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-08-4- 4"; volume: "117"; issue: "31"; pages: 
            "18459-18469"
COMMENT     Sequence Label: Mdn1-deltaC
FEATURES             Location/Qualifiers
     source          1..16587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      126..580
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(213..232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     422..443
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        606..710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(711..779,780..1571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(929..948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1742..2330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2231..2250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2484..2501
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2635..2859
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2926..3459)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3648..3653,3654..3670,3671..3676))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4028..4119
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4044..4061
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4186..4203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4225..4245
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    16201..16335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16238..16257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16292..16311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  16364..16529
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
       61 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga
      121 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
      421 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      481 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      541 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1081 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1141 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1861 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2101 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2221 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2281 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2521 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta
     2581 acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg
     2641 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     2701 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     2761 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     2821 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
     2881 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     2941 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     3001 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     3061 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     3121 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     3181 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     3241 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     3301 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     3361 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     3421 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
     3481 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     3541 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     3601 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     3661 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     3721 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
     3781 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
     3841 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
     3901 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
     3961 gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacgtata ctccggaata
     4021 ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
     4081 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
     4141 cccaccatcg ggcgcggatc tcggtccgaa accatgtcgt actaccatca ccatcaccat
     4201 cacgattacg atatcccaac gaccgaaaac ctgtattttc agggcgccat ggggatccgg
     4261 aattcaaagg cctacgtcga catggatgtg ttgattgagt gggttgcaat ataccctcag
     4321 atatatgata ttttggagca tattaattac gttccaagca acactttgca acgtttgcgt
     4381 ttgcaccaac cctggtcaaa aattgactac gacgtttggt ttttgtatgc atcggacgaa
     4441 attcgtgaaa cctgcaaagt caaatattat ggagaaacaa agacttacgg tgaagttttt
     4501 gttttagaaa atgaaagaat atcccaactt catcgtcttt ttgtttcttg gacggtctct
     4561 gaaagagcag aacatctaaa gaatttacta tttgacgctg gattatccaa tttacctctt
     4621 gttgaactag ggggaaatgt cttttttaac tctcacgtgc cgcttccttg ttccctagtt
     4681 ttaactaaaa gtacacagga aaatttaaat cgcataactc cttacttggt acaaaaaaga
     4741 ccaattcttc tggctggtcc agaaggaatt ggtaaaaagt ttttaatcac acaaattgct
     4801 gcgaaattgg gacaacagat cattcgtatt catcttagcg attccacaga ccccaaaatg
     4861 ttgattggaa catatacctc tccaaaacct ggagaattcg agtggcaacc tggtgtgttg
     4921 acacaagctg taattactgg aaaatggatc ttgtttacaa atatcgagca cgcaccttct
     4981 gaagtgctta gcgtcttgct tcctcttctc gaaaaaaggc aactcgtaat tccctcacgt
     5041 ggtgaaacaa tatatgctaa aggatccttt caaatgtttg ctacatcatc tatgaaaaca
     5101 aagattcttg ggcaacgatt atggcagata cttgatttga cttatcaacc agatgaatgt
     5161 gttgaggtag tatctactct atatcctgtt ttatcaatca tttgtcctac tttgtactct
     5221 gtttacaaag atatatttga tcttttttca caaagatcgt tccttgccac ctcaaaaata
     5281 tatcgtcgat tgtgtttgcg tgatttctac aaatttatta aaagggttgc gtttctatat
     5341 cacaaattta tgatcccttc cgaccatgta gtcatatctc aagaactcca agatgcagtt
     5401 tttaaagagg ctatcgatat gtttggtgcg tttattcctt ctagagacgg ttttgatctt
     5461 gtggtgcgta acgtcgctat agagttaaat ataccacctg aaaaggcttt acaattacga
     5521 tattctattc ctgtatttca aaatttggaa cataacataa acataggtag atgttcactt
     5581 aagaaattgt ctactattcg ttcttgttcc actaattcat atgctttcac atcttcatct
     5641 cttggcttat tggaacagct ggctgcaggt gttcaaacaa acgaaccatt gcttctagtt
     5701 ggtgagactg gtactggtaa gaccactact atccaacttt tagctggctt gttaggtcag
     5761 aaagttactg taattaatat gtctcaacag acggaatctt ctgatatgct tggcggttat
     5821 aaacctatca acgcttccac acttggtctt ccattgcatg agaggtttat agatatattt
     5881 gagcaaactt ttagttccaa gaaaaatgct aagtttatat ccatggcatc aacgtcagca
     5941 cgacggtttc gttggaaaac ttgcctaaaa atttggaaag aagcttgcaa attgtctaaa
     6001 acggttcttg atggtcaaca acctcttcct aatcctcaaa aaagacaaaa acgtttatct
     6061 aatcaggttg agcttcgaaa tcagtgggct aaatttgaga aagaggttga agattttgaa
     6121 aaagttctta cgggaggttc caatggcttc atgttttctt tcgttgaggg tgctttggtg
     6181 aaggcggttc gttccggaca ctgggtatta ctagacgaga ttaatttggc atcattagag
     6241 actttagaac ctattggcca acttttaagc tcatatgaat cgggtatact tctcagtgaa
     6301 cgtggcgata taactccaat tactcctcat aaaaatttta ggttatttgg ctgtatgaat
     6361 ccttctacag atgttggaaa acgtgaactt gagccatctt ttcgatcaag atttactgaa
     6421 atatatgttc attctcccga tcaaaattta gatgatttat tatcaatcat tcaaaagtat
     6481 atcggttctc tttgtattgg taatgaacat gttatccgag aggtcgctga actatatcaa
     6541 gttgcaaaga gtctttctct cgatggttct cttgttgatg gagctggtca aagaccacac
     6601 tataccgttc gaacattgag ccgaactttg tcgtatgtaa cggaaattgc tccaatttat
     6661 ggattgagaa gatcccttta tgagggattt tgtatgtctt ttttgacgtt actcgaccat
     6721 actagtgaaa gcttattgta caaccatgtg gttcgtttca cactaggtga gctaaatagg
     6781 gatcaacaga atgcaatact caagcaaatc cctaaagtac ccgatcattc tagttatatt
     6841 gctttttgcc attactggtt gagacgtggg tctttccccg tagaggaaca agagcattat
     6901 atcataacac catttgtgca aaaaaattta ttaaacatag ctagagcttg ttctactagg
     6961 atgtttccaa ttttaatcca aggaccaact tctagtggca aaacaagtat gattgaatat
     7021 gtagctaaaa agactggaca taagtttgtt cgaataaaca atcacgaaca cactgatcta
     7081 caggaatata ttggcaccta cgttactgat gataacgggt cattgagctt tagggaaggc
     7141 gttttggtgg aagctttacg aaatggatat tggattgtat tagacgaact caatctggcc
     7201 cctaccgacg ttttagaagc tttgaataga cttttagatg ataatcgtga gctttttata
     7261 cccgaaacac aagttttggt taaaccacat cctgagttta tgctttttgc aacacagaat
     7321 cctcctggtg tttatgctgg tcgtaaacat ctttctcgag cattccgcaa tagattctta
     7381 gaaattcatt ttgatgacat tcctgaaaat gaactggaga cgattcttca taagcggtgt
     7441 aaaatagccc cttcatatgc tgcaaagatt gtacaagtat tccgcgagct atctcttcga
     7501 agacaaacca cccgaatttt tgagcaaaaa aattcttttg ctactttgag agatttattt
     7561 agatgggcgt ttcgcgaagc tgtcggctac caacagttag cagaaaatgg ttatatgctt
     7621 ttggctgaac gcgcgagaga ccaaaaggac aaactggccg tgcaagaagt aatcgaaaaa
     7681 gttatgaagg taaaaattga cactgatggt atttataacc ttgactctat ggagatcttc
     7741 caggatatga gtttaaaaga gggtccttta tctaaggttg tttggacacg tccaatgatt
     7801 cgtttatttt gcctcgtatg gcgctgttta ctagcaaaag aacccgtttt gttagttgga
     7861 gataccggtt gtggtaaaac aactgtttgt cagattctag ctgagtgttt gcacaaagag
     7921 ttgcatatta ttaatgctca tcaagatacg gaaaatggag acataattgg tgctcaaagg
     7981 cctgtaagga atcgttccgc tgtaaattac agtcttcatt cacaattatg tgaaaaattt
     8041 aacgtacaag aaagtctgga ttctattgac gatctcattg agaaatttga aaaactttct
     8101 tcttctgaaa agaacgacaa tttatctaat ctgatcgaac gccagattat caagtaccgc
     8161 tctctgtttg aatggcatga cggtgctttg gttaccgcga tgaagcaagg cgactttttt
     8221 ttacttgatg agattagtct cgccgatgat tccgttttgg agagattgaa tagtgttctt
     8281 gaattatctc gtactcttac tttagtggaa cacagtaatg ctgccgtatc tcttactgcc
     8341 aaagatggat ttgcattttt tgctactatg aatcctggtg gtgattacgg taagaaggaa
     8401 ctttcgccag cgttgcggaa tcggtttacc gaaatttggg ttccaccgat ggtcgataca
     8461 gaggacattc ttaaaattgt cgaaggcaaa cttcataaca acaaaatcga gttagctcgc
     8521 cctttggttg aatatgcgaa atggcatgcg aatgaatact tgtatactga tgtgatttct
     8581 attcgtgatg tcttatccgc agtagaattt ataaatgcat gcgaaatttt ggatttaaat
     8641 ctggtattgt ttaatgctgt ttcaatggtt tttatagatg cgcttggttc tttcacgacc
     8701 ttttctttaa gtaacaattt ggcatcctta cacgctgaac gtcaaagatg tttcgctaaa
     8761 ttaaatgagt tagcaggatc taacattatg gcatccaaat ctgccgatat atcaattaaa
     8821 ttctccgact catcattttt tataggtgat tttggaatac ctttaggaga ttctgttgaa
     8881 tcggattcga cttatagttt acatacagat actactttaa tgaacgcttc caaggttttg
     8941 agggctctgc aggttctgaa acctatttta ctggaaggta gtcctggagt tggcaaaacc
     9001 agtcttatta cagcacttgc tcgtgaaaca ggacatcaac tggtgcgtat aaatttgtct
     9061 gatcaaacag atttgatgga tcttttcggc tcagacgttc ctgtggaagg tggtgaagga
     9121 gggcagtttg cttggcgtga tgctcctttt ttggcagcga tgcgtaatgg tcattgggtg
     9181 ttgcttgatg aattaaattt agcttcacag tcagtcttgg aaggcttaaa tgcttgtctt
     9241 gatcatcgta atgaagctta tataccggaa ttagacaagg tgtttaaagc tcatccgaat
     9301 tttcgagttt tcgctgccca aaatccacag catcagggtg gcggtaggaa aggcttaccg
     9361 aggtcgttca tcaatagatt ttcggttgtt tacgttgagg ctcttaaaga aaaggacatg
     9421 attgaaattg cagcatgtaa ctatcatcaa gtaaatgagg attggcgttt gaagattatc
     9481 aagtttatgt ttcgtttgca agacaatatt gagaaagaca ttagttttgg tagttttgga
     9541 agcccttggg aatttaatct tcgtgatact ttacgttggt tacaactttt gaatgatgct
     9601 ccaaaatata catgtgtttc acctgctgat tatttggaag tcatggtcct gcatcgtatg
     9661 agaacggttg aagaccgtgt tagaacatgc gaattgttta aagaagtatt tgatatagat
     9721 tatgagcccc gtactattgg ttttagcttg agcagccaat gttttaaagt cggacattca
     9781 cttcttgtca gggatgtcga aagacagaaa actttattgg acagtcaaaa tatacttcaa
     9841 tcacaattac cagttctcga atcggtaata acatgcatca acaaaaaatg gccctgtatt
     9901 ttggtgggcg acactgccac agggaaaacg tgcattctcc gtttgcttgc ggctattgct
     9961 ggtgctaaaa ttaaagaaat ggctgttaat tccgacactg atacaatgga ccttattggt
    10021 gagtatgagc agatagatat atctcgaaaa gcatcagagc tttttacgga tctttctcaa
    10081 caattattaa atattgtcat caaatatagg aatttcgata atattttccg tgaaacttcc
    10141 ttatatacat taacgacgac ttctttcaaa acacactccc aagcgtttac actgcttcaa
    10201 aaagtagtgg accaattgga tcagttaaag atacatgaaa ccctagtaca ttctttaggt
    10261 gatattcatg aaaaggctcg taaactgctt gcagagtttt cagcttctcc agcaggtcgg
    10321 tttgaatggt ttgatggcta tttactaaag gctgttgaag aagggcattg gtttgtcttg
    10381 gataacgcta atttatgtag tcctgctgtc cttgatcgcc taaactcatt gctagaacat
    10441 aaaggagtct taattgtcaa tgagaaaact actgaagatg gacatcccaa aaccataaag
    10501 ccgcatccca actttcggtt gtttttaacc gtaaatcctg tttacggtga actatcacga
    10561 gcaatgagga ataggggggt ggaaatattc ttgttaaaag aagcccttac cgagattgac
    10621 aagaagcaaa tgtctcttct agaacctgca ccgatatcat ctgctgtgga tacgcttgct
    10681 tcaaatattt cgtacatcaa atatgttttt gaaaccatgg gtaaaattga aatagatggt
    10741 aattatatgt acattgccca tgctataatc cttgcgttat tttctccaag gcaactaaaa
    10801 cttcttcgaa aagttttatt aactaatcct caattttcgt tatctatcaa agctgatgca
    10861 gaacttttgc ttaccttaaa aaatttggta caaaaaatat attgcgctga ttattttaat
    10921 catatggatt taaaggcttc gaggttcatg gatatatatg aatatcccgt ccagcttcgt
    10981 gaagttgtag gcctgataca aacgattaat gattttcaat cagtcatatt aacctcccat
    11041 ttggagttgc ctgagacata tgcatcaggt ttattgtttg tttctgcaca tgaaattctc
    11101 gacttaacag aggaagtcaa ccgcttagca gtttccacaa gcaatagtac ctaccttctc
    11161 aaaagtgcgt ctgctgttta tcataacgtt tcttctttta aagggtctac tccttctttg
    11221 tggaacttat taaatcagtt ttctaaattt ttgattgaaa ttgcaagtgc aaactcaaac
    11281 attgtctata agctatccta cgacgttatt cgtcattttc tcaaactagt tgtattgtgg
    11341 aaaaatatat acgtttggac aaatgttccc gattgtgata ttagtagatt ttattgttac
    11401 actaaaatgt taggagaatg gatgtttact ttaacggaaa aaacaaagct attggagtca
    11461 tttttaccta aggattcgct tgaaaaattt tcagaattgc aaaacttgtc tactgggtta
    11521 cacatgcaag caatctggga caaatggcat gctttcgttc ctcgtacata tgatcaatgg
    11581 tcgctttgga atacagttga taaactattg acacaatatg ttaatgccaa catcccatca
    11641 atttctatgg aaactacggc atgtgaagtt gtaggaactt ctttatcact attaaacaaa
    11701 gttctggttg aaaacgaagt tggagacatt tattcatatc tcaaaatatt gggtaaaggt
    11761 gttaatgagc taaaatcttc taaacaagtt attttacccg aaaaccttgt taatcttttt
    11821 aactgtttag cttcattgga tttgttgcat atttttatca aatatactac ctcctcgttc
    11881 tttctaaccg acgattttgt gcgttttata cgagtttgct ttcattccag aatttcaggt
    11941 aatctgctga cgttactgca tggaattagt ttcgatagca cgaaagctgt agcaccagtt
    12001 ttaacctact tcgatttttg ctctttaacc accggcaaca tcttaggtcg tattgcactt
    12061 gcttttacca gcattgatga gaatgcaaat ttggagtctg caaatatttt tgagcatgca
    12121 cgattggcct tacttcagca tttcatggat cactcttctt tattagcgga ggactcatcc
    12181 acaaaaatga atttgatatt gctacagaga tatgctgtta taatttcaat attcttggat
    12241 caaggaaagt gtgaaaaggc caatgacctt ataacgaagc tctctcttcc ttatgaggaa
    12301 ttagctgaaa actttgtctc tatattagaa gcttgcaaag catttttggt tgccaattca
    12361 gaattcattt cttacacgta tactgagcgt tttattcatt ctttgagatt tctgaaagac
    12421 tcttggttaa gcagcaacca acagaaaatg ttgaaaaatc aaggaatggc ttatatttat
    12481 tttgcttctg gaatgctgtt agtatatgtt ccagacaaac cctttgatcc tgccctgctt
    12541 cctttgctca cagtagaaag tttgagacat taccttgaaa gtctctataa ggaaagccaa
    12601 attttagaaa ttgccgagtc cttgaattca ggaaaagtta atagcgtgat gagacgttta
    12661 gtttcgactg aaatttcgaa cacgcctaac attgattctt ctttcagtac cgtgtacaga
    12721 tcactgaatg agagcatagt tcctctttat agtgaattgg aatttttcat gaagtctgtt
    12781 gtacttaatc agtacatatt tgagctagca atgcggcttt ctaaagaatc aaatatcgct
    12841 gttgttgaag aagctaaatc ttttgtaaca aaatggaagg cctatattga aagaatacgt
    12901 gaggcttatc ctcaatttgt tgatgtttac gagctcattc tttcattcat aagtttcatg
    12961 atatacggaa tagagctgct tatgttcgaa gctaaacgac gcttggatga acgatctcag
    13021 atactctcaa cgcttatttt gacattagtg gatccttcaa gttttgcacg ttcgcttagc
    13081 tttgatgatg tttcaaattt aattgagcag atcaaagtgc ttgatttaaa tgattcaatc
    13141 agatttgaaa tttacttatt cttagcttct cgcttatgta gtgaaaaaca gcattctagt
    13201 gatactcatt cgttggccaa ttctttcgta ctattagcta atgaatttta catccataat
    13261 gcaaaaatta agcagaaaga gctcgaggag atagaagaaa aaaacagact ttatcgacag
    13321 agagaattca attttgataa gaatgattac ttaaaagtgt tcattaatta cgacgatgaa
    13381 gttgaacctg aagttgaacc tgaagtcgta attgaaagaa aaagattctt acaactgcaa
    13441 ttcgcttttt ggtctctcta taacgaaata tattctgaga aaatgaacgt aataccgttg
    13501 gagcagctta tgaataccgg gtcatatctt gctaagaaaa ttaaagttaa gaatccagac
    13561 atgattgctt catcaggttt tgatattgtt agcgttgtac tcatgatggg tgttaaatcg
    13621 actaacgaaa gacagtattg gaccccacct gtttacaatt tttattctga tccgaaccct
    13681 tcaaaagcta tagaagttcg cgatttgatc aaaattgttg aaagccgagc tatatcttta
    13741 atcaaaaact ggcctgagaa ttttgttcta agaggcttaa aagatgccat tgatgctata
    13801 cttaacttaa gtccgttttc tcctattgct gaatatctat caaaacttga acgagtgttt
    13861 catttactat ctgaatggga aaagttggct agtagagaat attcgcttgc taatgaaatg
    13921 gacttgatta agaaaaaaat cattgattgg agaaagtttg agctttcgaa ttggaacaat
    13981 ttgcttaaat tagaagaata caaactttct gagagggttt atccaagatt gtatagtatc
    14041 ctgcaattta ttatactgaa gcctttcttt gagaatagca aattcactaa gcaaaatttg
    14101 tgcgaatctg cctctattat agttcagttt atcaccgatt tgactgttgg ggagtttcaa
    14161 ctttgtttga aatgcttgtt atcattttct caacatgctg cttctttaag aatatgccac
    14221 gggattgatg caatgctcct aaatatctat cattactttg agcaattttt atccaaagtt
    14281 tctgaggcaa ttcatacaca aaaacagtcg cttgaaaatt caatcaaaga gcgaatacta
    14341 ttgatgagtt ggaaggatac caatgtttat gcgttgaagg aaagtgctaa aaagagtcat
    14401 gctgagttat ttaaagttct acataggtat agagaagttt tgagacagcc tgtttcttca
    14461 tatctttcgc aaaaacatga ttgggattcc ttacttgaca ctgaaaacaa ttcagcaatg
    14521 tgggttgcca aaaaagtaaa ccttagtccc tcatatattg aaaaaatgga tacagaaatt
    14581 atgaagctgg ttccagtacg gttttctaat actccgacta ctctaaggtt gatgtggact
    14641 ttatttgcta acgttgaaaa gcctggttca actttcacta atatggtttc aaatttaatt
    14701 actgatgccc gtgagctgat gaagctcaca ccggaaacca ttaacgatga taatctgtct
    14761 gaaattaagc atttgaaaag tcgaaagcat ttacttctta ctgaaacatt caaaactcta
    14821 aaggcttttg gcttgcaata ccgtgttaaa gctggtattg aggaaaatct tagtaattta
    14881 cgaaatttgc tggcagttat tcctactttt ccagttactt cattgagtat agaaaaagtt
    14941 gatcgttcac tgatgaaatc attagacttt attcccaaat ttcagacact agctggtcat
    15001 caacataatg atttaagtgt tcctgaagtt caaaaaggtg taggtttatt taacagtatg
    15061 ttatctttgc agttaggtga gcgtgcacag ctagtcgaat ttaccaatga attattggcc
    15121 ttaaaaaatg tttattcaga agttggtgtg aatggttcgc cgttggaatc ctttaataat
    15181 tcttcattta atgaggtgag tagtctggga tatgatcacg atttcgaaaa tcgagcgcag
    15241 gctgtctcta tgctttgtca aatctatgcc atagttatac agaagcactc aagtatatct
    15301 cccactgctt catttcaatc gatcgggcat gaacttagtc gattcgccga cctgctatct
    15361 aacaagttgt ttccttcctc aattccgctt tatgcatctg ctgataaagt ttcttcgatt
    15421 cgtgatcaac agaaaggaat aaatgatttg attgaatact gtcgcaaaaa aaggacagaa
    15481 ttgccggagt tgtcttactg ctttaaacat ttggttagct tgcaaagttt gaagtcaata
    15541 tccagaacgc aagtggattt aacaaatgac gagtttttga atttgatgaa ctttgtttta
    15601 aacttatttg atagtttgct ttcttcaata gaaaccgcta caaagaatat gcgaacattc
    15661 aaagaactag ccgaaacgtc tagttttatc gaaatgagca gttgcttttc taaagttttg
    15721 cgagctttta atttaaagtt tcagtctatg aagctgagtt ctttgaaaga gaagttacgt
    15781 tcttcttctg tcgataaaat gagttgtcaa ctacttatgc tttttcttcc tgtttgtgag
    15841 cagtttataa acttagcgga aagtgtgcta gattacttta taaatgttca taactcaaac
    15901 cttgattcgc tatccaaaat ttcgacgttg tttttcatgg ttgcaaacaa tggtttctgc
    15961 tcgcctgacc ttccacaaga aggaaaatca aatagtggcg aattagagtc tggttaaccc
    16021 ggggcggccg ctttcgaatc tagagcctgc agtctcgagg catgcggtac caagcttgtc
    16081 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
    16141 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    16201 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    16261 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    16321 tatcatgtct ggatctgatc actagatctg cctaggagat ccgaaccaga taagtgaaat
    16381 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
    16441 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
    16501 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
    16561 atcaaacatc ctgccaactc catgtga
//
