LOCUS       sequence-337864-        7929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression a mutated 3'UTR of the murine Arid3a 
            gene after a GFP, to evaluate miRNA binding. Regions not containing 
            miR-125b or let-7c binding regions not included..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(141..159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(169..186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(169..185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(178..200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      327..782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(414..433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     624..645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        808..912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(913..981,982..1773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1131..1150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1944..2532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2433..2452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2686..2703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2820..2841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2856..2861,2862..2879,2880..2886)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2894..2910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2899..2921
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2918..2934
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2918..2934
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2953..2973
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2955..2973
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3018..3397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        3454..3645
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3477..3497)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3664..3890
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3891..4071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4118..4243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4736..4969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5154..5198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5411..5528
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5598..5809
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5826..5835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5832..5834,5835..5837,5838..6551)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5832..5834,5835..5837,5838..6548)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5877..5898)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6138..6157)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6485..6506
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6782..7370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6835..6855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7253..7264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7442..7675
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7753..7874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7790..7809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7844..7863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(7914..7929,1..120)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
       61 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      121 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      181 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      241 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      301 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      541 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      661 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      721 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      781 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2701 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2761 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2821 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2881 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2941 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcggta
     3001 ccgtcgacct gcaggttgac attgattatt gactagttat taatagtaat caattacggg
     3061 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3361 gcagtacatc tacgtattag tcatcgctat taccatggcg cgccatgcag ggcctgaaat
     3421 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
     3481 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3541 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3601 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccttaag aggcgcgcct
     3661 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     3721 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     3781 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     3841 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     3901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4081 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4141 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4201 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4261 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4321 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4381 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4441 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4501 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4561 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4621 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4681 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     4741 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     4801 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     4861 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     4921 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     4981 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5041 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5101 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5161 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5221 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5281 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5341 ttcagaccca cctcccaacc ccgaggggac aattctagat cttgagacaa atggcagtat
     5401 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     5461 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     5521 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgagttgg
     5581 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     5641 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     5701 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     5761 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg
     5821 gatccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5881 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5941 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6001 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6061 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6121 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6181 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6241 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6301 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6361 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6421 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6481 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6541 tgtacaagta aacatgtcgc acgtctgttt ccatgatgaa tcccggcaca tatttgtgtt
     6601 cattaggtga aacagacttt ttttagggta actggctagg tgatatagca gccagcgcaa
     6661 gtttctttct ccatgaggtc ttttgacacc tggtgtcgca tagcttggcg ttgagacacc
     6721 caaacgcata gcaaagaact gttttcttgc gctggtcagg cgtaaaacgc gttaagtcga
     6781 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6841 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6901 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6961 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7021 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7081 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7141 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7201 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7261 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7321 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     7381 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7441 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     7681 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7741 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7801 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7861 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     7921 ccctaactc
//
