LOCUS       sequence-337865-        8374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of miR-99a with GFP as a reporter 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.GFP.miR-99a
FEATURES             Location/Qualifiers
     source          1..8374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    65..186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(102..121)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     156..175
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      226..361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(381..400)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(382..400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(410..427)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(410..426)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(419..441)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      568..1023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     865..886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1049..1153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1154..1222,1223..2014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1372..1391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2185..2773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2674..2693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2927..2944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3061..3082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3097..3102,3103..3120,3121..3127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3135..3151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3140..3162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3159..3175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3159..3175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3194..3214
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3196..3214
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3259..3638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        3695..3886
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3718..3738)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3905..4131
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4132..4312
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4359..4484
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4977..5210
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5395..5439
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5652..5769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5827..6228
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      6239..6248
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6245..6247,6248..6250,6251..6964)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6245..6247,6248..6250,6251..6961)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6290..6311)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6551..6570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6898..6919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7468..8056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7521..7541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7939..7950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8128..8361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
       61 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      181 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
      241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      361 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
      421 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      481 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      541 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      961 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1021 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3001 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3061 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     3121 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     3181 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagcggt
     3241 accgtcgacc tgcaggttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3301 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3361 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3421 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3481 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3541 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3601 ggcagtacat ctacgtatta gtcatcgcta ttaccatggc gcgccatgca gggcctgaaa
     3661 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     3721 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3781 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3841 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccttaa gaggcgcgcc
     3901 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     3961 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     4021 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     4081 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     4141 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4201 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4261 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     4321 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     4381 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     4441 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     4501 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     4561 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     4621 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     4681 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     4741 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     4801 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     4861 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     4921 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     4981 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     5041 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     5101 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     5161 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     5221 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     5281 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     5341 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     5401 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     5461 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     5521 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     5581 tttcagaccc acctcccaac cccgagggga caattctaga tcttgagaca aatggcagta
     5641 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     5701 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     5761 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagcga
     5821 gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc
     5881 agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga
     5941 gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc
     6001 cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc
     6061 atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa
     6121 ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga
     6181 gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgga tcgcggccgc
     6241 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6421 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6661 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6721 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6781 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6841 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6901 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6961 gtgaggatcc gctagctgca tccttagaac tcagcattta aaacatttat actaaaggat
     7021 caatattatt tttgactctt aattgcatca gatatttaca acaaattcta tattaatagg
     7081 gggcccatgc aagatgttgc ccattggcat aaacccgtag atccgatctt gtggtgaagt
     7141 ggaccgcaca agctcgcttc tatgggtctg tgtcagtgtg gtaatctgac aaaatgctat
     7201 acacagtgcc gttcaacaat agttcagtaa aatcctgtta aactccagtt gattatatac
     7261 ttttgaagtc attatatttt cctttgtttt taatgtttat tttaatcatt gtctgtctta
     7321 caaggcaggc ttcaattctc aacaacttgg aagcgtttat atcacaccca ttcaagttcg
     7381 attccatgta cagtaaattg cataagaaag ttgaaccttt atagcagggt ttggaccagg
     7441 accggtaacg cgtcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7861 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8041 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8101 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8161 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8221 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8281 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8341 cagtgtggaa aatctctagc agtacgtata gtag
//
