LOCUS       sequence-337866-        8330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of let-7c with dTomato as a reporter 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.dTomato.let-7c
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    630..863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1048..1092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1305..1422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1480..1887
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1887..1896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1893..2594
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2026..2044)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2294..2313
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2488..2511
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3077..3665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3130..3150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3548..3559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3737..3970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4048..4169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4085..4104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4139..4158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4209..4344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4364..4383)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4365..4383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4393..4410)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4393..4409)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4402..4424)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4551..5006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4638..4657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4848..4869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5032..5136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5137..5205,5206..5997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5355..5374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6168..6756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6657..6676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6910..6927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7044..7065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7080..7085,7086..7103,7104..7110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7118..7134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7123..7145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7142..7158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7142..7158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7177..7197
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7179..7197
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7242..7621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        7678..7869
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7701..7721)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7888..8114
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8115..8295
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
       61 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      121 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      181 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      241 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
      301 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
      361 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
      421 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
      481 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
      541 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
      601 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
      661 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
      721 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
      781 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
      841 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
      901 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
      961 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     1021 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     1081 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     1141 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     1201 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     1261 ggacaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     1321 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     1381 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     1441 agcagagatc cactttggcg ccggctcgag cgagctgcag taacgccatt ttgcaaggca
     1501 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     1561 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     1621 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     1681 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     1741 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     1801 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     1861 gtcctccgac agactgagtc ggatccgcca ccatggtgag caagggcgag gaggtcatca
     1921 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     1981 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     2041 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     2101 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     2161 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     2221 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     2281 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     2341 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     2401 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     2461 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     2521 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     2581 acgagctgta caagtgaagt acaagtgagg atccgcaggt tagatggtca gaagacattt
     2641 tacgtgacct atgctggaaa cccaagtgtt taggttagtc ctatgagaaa cagttagcag
     2701 caaagtttac tggtaaaatg ttagaatatt atatctatat ccttgccaag cccttaggtg
     2761 tatggctgcc atatttggag gagctgactg aagatatgat aaggagtttg aagcaacatt
     2821 ggaagctgtg tgcatccggg ttgaggtagt aggttgtatg gtttagagtt acaccctggg
     2881 agttaactgt acaaccttct agctttcctt ggagcacact tgagccgtcg aggaattctt
     2941 catcacttta acctgattga gccaatttgt gtgcaagaag gtaatgtgtc atgagtatct
     3001 tggatcattg atttgggcct ttattacatt aaaatgatca tttcagccct attaccggta
     3061 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3121 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3181 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3241 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3301 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3361 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3421 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3481 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3541 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3601 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3661 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3721 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3781 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     3841 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3901 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3961 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     4021 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4141 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4201 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4261 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4321 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     4381 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4441 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4501 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4561 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4621 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4681 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4741 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4801 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4861 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4921 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4981 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5101 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5161 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5221 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5281 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5341 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5401 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5461 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5521 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5581 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5641 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5701 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5761 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5821 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5881 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5941 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6001 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6061 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6301 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6781 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6841 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6901 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6961 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7021 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7081 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7141 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     7201 caaaagctgg agctgcaagc ggtaccgtcg acctgcaggt tgacattgat tattgactag
     7261 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     7321 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     7381 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     7441 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     7501 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     7561 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     7621 ggcgcgccat gcagggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     7681 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7741 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7861 catagtccct taagaggcgc gccttaatgt agtcttatgc aatactcttg tagtcttgca
     7921 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7981 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     8041 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     8101 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8161 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8221 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8281 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
//
