LOCUS       sequence-337869-        8225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of miR-ctrl with GFP as a reporter 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.GFP.miR-ctrl
FEATURES             Location/Qualifiers
     source          1..8225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             158..338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    385..510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1003..1236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1421..1465
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1678..1795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1853..2254
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2265..2274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2271..2273,2274..2276,2277..2990)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2271..2273,2274..2276,2277..2987)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2316..2337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2577..2596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2924..2945
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           3000..3121
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3149..3163
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3191..3317
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    3345..3933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3398..3418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3816..3827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4005..4238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4316..4437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4353..4372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4407..4426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4477..4612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4632..4651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4633..4651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4661..4678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4661..4677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4670..4692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4819..5274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4906..4925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5116..5137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5300..5404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5405..5473,5474..6265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5623..5642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6436..7024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6925..6944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7178..7195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7312..7333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7348..7353,7354..7371,7372..7378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7386..7402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7391..7413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7410..7426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7410..7426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7445..7465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7447..7465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7510..7889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        7946..8137
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7969..7989)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(8156..8225,1..157)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
       61 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      121 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      181 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      241 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      301 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      361 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      421 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      481 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      541 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      601 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      661 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      721 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      781 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      841 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      901 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
      961 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1021 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1081 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1141 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1201 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1261 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1321 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1381 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1441 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1501 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1561 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1621 cccaaccccg aggggacaat tctagatctt gagacaaatg gcagtattca tccacaattt
     1681 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1741 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1801 ttattacagg gacagcagag atccactttg gcgccggctc gagcgagctg cagtaacgcc
     1861 attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg
     1921 tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc
     1981 ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca
     2041 gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca
     2101 ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct
     2161 tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc
     2221 actcggcgcg ccagtcctcc gacagactga gtcggatcgc ggccgccacc atggtgagca
     2281 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2341 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2401 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2461 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2521 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2581 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2641 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2701 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2761 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2821 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2881 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2941 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga ggatcctgtt
     3001 tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg
     3061 gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg
     3121 agcgatctcg cttgggcgag agtaagtagt gaagccacag atgtacttac tctcgcccaa
     3181 gcgagagtgc ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta
     3241 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
     3301 ataaattaaa tcactttacc ggtaacgcgt cgcgttaagt cgacaatcaa cctctggatt
     3361 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3421 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3481 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3541 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3601 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3661 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3721 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3781 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3841 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3901 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     3961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4021 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     4261 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     4321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4441 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     4621 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     4681 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4741 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4801 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4861 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4921 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4981 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5041 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5101 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5161 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5641 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5941 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7201 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7261 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7321 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7381 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7441 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagcgg taccgtcgac
     7501 ctgcaggttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     7561 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     7621 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     7681 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     7741 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     7801 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     7861 tctacgtatt agtcatcgct attaccatgg cgcgccatgc agggcctgaa ataacctctg
     7921 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     7981 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8041 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8101 aaagcatgca tctcaattag tcagcaacca tagtccctta agaggcgcgc cttaatgtag
     8161 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     8221 caagg
//
